The bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16S rRNA genes.
(2003) In International Journal of Food Microbiology 83(3). p.245-252- Abstract
- The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows... (More)
- The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows that the overall pattern of the initial and chill-stored pork flora, as shown by a molecular approach, was in agreement with results obtained in previous studies using traditional cultivation methods. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128295
- author
- Olsson, Crister LU ; Ahrné, Siv LU ; Pettersson, B and Molin, Göran LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Pork, Eperythrozoon, Diversity, 16S rDNA cloning
- in
- International Journal of Food Microbiology
- volume
- 83
- issue
- 3
- pages
- 245 - 252
- publisher
- Elsevier
- external identifiers
-
- wos:000183055800002
- scopus:0038078459
- ISSN
- 0168-1605
- DOI
- 10.1016/S0168-1605(02)00372-0
- language
- English
- LU publication?
- yes
- id
- e5be906e-dded-42a9-921c-90e0b4623249 (old id 128295)
- date added to LUP
- 2016-04-01 11:43:06
- date last changed
- 2023-09-01 04:34:20
@article{e5be906e-dded-42a9-921c-90e0b4623249, abstract = {{The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows that the overall pattern of the initial and chill-stored pork flora, as shown by a molecular approach, was in agreement with results obtained in previous studies using traditional cultivation methods.}}, author = {{Olsson, Crister and Ahrné, Siv and Pettersson, B and Molin, Göran}}, issn = {{0168-1605}}, keywords = {{Pork; Eperythrozoon; Diversity; 16S rDNA cloning}}, language = {{eng}}, number = {{3}}, pages = {{245--252}}, publisher = {{Elsevier}}, series = {{International Journal of Food Microbiology}}, title = {{The bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16S rRNA genes.}}, url = {{http://dx.doi.org/10.1016/S0168-1605(02)00372-0}}, doi = {{10.1016/S0168-1605(02)00372-0}}, volume = {{83}}, year = {{2003}}, }