Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow fractionation coupled to multiangle light scattering
(2017) In Journal of Chromatography A 1506. p.138-141- Abstract
Starch and hence, amylopectin is an important biomacromolecule in both the human diet as well as in technical applications. Therefore, accurate and reliable analytical methods for its characterization are needed. A suitable method for analyzing macromolecules with ultra-high molar mass, branched structure and high polydispersity is asymmetric flow field-flow fractionation (AF4) in combination with multiangle light scattering (MALS) detection. In this paper we illustrate how co-elution of low quantities of very large analytes in AF4 may cause disturbances in the MALS data which, in turn, causes an overestimation of the size. Furthermore, it is shown how pre-injection filtering of the sample can improve the results.
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https://lup.lub.lu.se/record/fd8c4fcf-614d-42e4-8a75-b63ce263bd75
- author
- Pérez-Rea, Daysi LU ; Zielke, Claudia LU and Nilsson, Lars LU
- organization
- publishing date
- 2017-07-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amylopectin, Asymmetric flow field-flow fractionation (AF4), Macromolecules, Multiangle light scattering (MALS), Root-mean-square radius (r), Size distribution
- in
- Journal of Chromatography A
- volume
- 1506
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:28551020
- wos:000403990300017
- scopus:85019620178
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2017.05.029
- language
- English
- LU publication?
- yes
- id
- fd8c4fcf-614d-42e4-8a75-b63ce263bd75
- date added to LUP
- 2017-06-13 13:10:29
- date last changed
- 2024-09-16 03:12:41
@article{fd8c4fcf-614d-42e4-8a75-b63ce263bd75, abstract = {{<p>Starch and hence, amylopectin is an important biomacromolecule in both the human diet as well as in technical applications. Therefore, accurate and reliable analytical methods for its characterization are needed. A suitable method for analyzing macromolecules with ultra-high molar mass, branched structure and high polydispersity is asymmetric flow field-flow fractionation (AF4) in combination with multiangle light scattering (MALS) detection. In this paper we illustrate how co-elution of low quantities of very large analytes in AF4 may cause disturbances in the MALS data which, in turn, causes an overestimation of the size. Furthermore, it is shown how pre-injection filtering of the sample can improve the results.</p>}}, author = {{Pérez-Rea, Daysi and Zielke, Claudia and Nilsson, Lars}}, issn = {{0021-9673}}, keywords = {{Amylopectin; Asymmetric flow field-flow fractionation (AF4); Macromolecules; Multiangle light scattering (MALS); Root-mean-square radius (r); Size distribution}}, language = {{eng}}, month = {{07}}, pages = {{138--141}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow fractionation coupled to multiangle light scattering}}, url = {{http://dx.doi.org/10.1016/j.chroma.2017.05.029}}, doi = {{10.1016/j.chroma.2017.05.029}}, volume = {{1506}}, year = {{2017}}, }