Nano-Infrared Imaging of Primary Neurons
(2021) In Cells 10(10). p.1-15- Abstract
- Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM)... (More)
- Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid β-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect Aβ-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists’ awareness of the potential of s-SNOM as a tool for analyzing amyloid β-sheet structures at the nanoscale in neurons without the need for immunolabeling (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0dab6a6b-e62e-44a0-9586-ac14066cc3b9
- author
- organization
-
- Medical Microspectroscopy (research group)
- NanoLund: Centre for Nanoscience
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- Neuroinflammation (research group)
- Experimental Dementia Research (research group)
- Solid State Physics
- Department of Experimental Medical Science
- LINXS - Institute of advanced Neutron and X-ray Science
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- O-PTIR, s-SNOM, Alzheimer’s disease, neuron, amyloid-beta
- in
- Cells
- volume
- 10
- issue
- 10
- article number
- 2559
- pages
- 1 - 15
- publisher
- MDPI AG
- external identifiers
-
- scopus:85115787740
- pmid:34685539
- ISSN
- 2073-4409
- DOI
- 10.3390/cells10102559
- language
- English
- LU publication?
- yes
- id
- 0dab6a6b-e62e-44a0-9586-ac14066cc3b9
- date added to LUP
- 2021-09-28 23:13:38
- date last changed
- 2023-11-08 19:59:33
@article{0dab6a6b-e62e-44a0-9586-ac14066cc3b9, abstract = {{Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid β-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect Aβ-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists’ awareness of the potential of s-SNOM as a tool for analyzing amyloid β-sheet structures at the nanoscale in neurons without the need for immunolabeling}}, author = {{O. Freitas, Raul and Cernescu, Adrian and Engdahl, Anders and Paulus, Agnes and E. Levandoski, João and Martinsson, Isak and Hebisch, Elke and Sandt, Christophe and Keppler Gouras, Gunnar and Prinz, Christelle and Deierborg, Tomas and Borondics, Ferenc and Klementieva, Oxana}}, issn = {{2073-4409}}, keywords = {{O-PTIR; s-SNOM; Alzheimer’s disease; neuron; amyloid-beta}}, language = {{eng}}, number = {{10}}, pages = {{1--15}}, publisher = {{MDPI AG}}, series = {{Cells}}, title = {{Nano-Infrared Imaging of Primary Neurons}}, url = {{http://dx.doi.org/10.3390/cells10102559}}, doi = {{10.3390/cells10102559}}, volume = {{10}}, year = {{2021}}, }