Size, structure and scaling relationships in glycogen from various sources investigated with asymmetrical flow field-flow fractionation and 1H NMR
(2011) In International Journal of Biological Macromolecules 49(4). p.458-465- Abstract
- In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching 1H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that... (More)
- In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching 1H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that glycogen is a polysaccharide showing rather diverse conformational properties, over the size distribution, depending on its origin and the extraction procedure. The ratio between root-mean-square radius and hydrodynamic radius varies depending of both sample origin the molar mass of the macromolecules, reflecting differences in conformation and scaling within the size distribution. Thus, a priori assumptions regarding the rrms/rh are difficult to make and rrms/rh based on average properties give an incomplete description of the properties. Furthermore, the results display the strength of the apparent density (as obtained from AsFlFFF-MALS-RI) as a characterization parameter for scaling in disperse macromolecules. © 2011 Elsevier B.V. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2208328
- author
- Fernandez, Celine LU ; Rojas, Carola LU and Nilsson, Lars LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Field-flow fractionation, Conformation, Glycogen
- in
- International Journal of Biological Macromolecules
- volume
- 49
- issue
- 4
- pages
- 458 - 465
- publisher
- Elsevier
- external identifiers
-
- wos:000295502500003
- pmid:21640751
- pmid:21640751
- scopus:80052275025
- ISSN
- 1879-0003
- DOI
- 10.1016/j.ijbiomac.2011.05.016
- language
- English
- LU publication?
- yes
- id
- 00eac4d3-30ab-4162-a005-408ef2e068fd (old id 2208328)
- date added to LUP
- 2016-04-01 10:11:39
- date last changed
- 2023-11-24 03:04:59
@article{00eac4d3-30ab-4162-a005-408ef2e068fd,
abstract = {{In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching 1H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that glycogen is a polysaccharide showing rather diverse conformational properties, over the size distribution, depending on its origin and the extraction procedure. The ratio between root-mean-square radius and hydrodynamic radius varies depending of both sample origin the molar mass of the macromolecules, reflecting differences in conformation and scaling within the size distribution. Thus, a priori assumptions regarding the rrms/rh are difficult to make and rrms/rh based on average properties give an incomplete description of the properties. Furthermore, the results display the strength of the apparent density (as obtained from AsFlFFF-MALS-RI) as a characterization parameter for scaling in disperse macromolecules. © 2011 Elsevier B.V.}},
author = {{Fernandez, Celine and Rojas, Carola and Nilsson, Lars}},
issn = {{1879-0003}},
keywords = {{Field-flow fractionation; Conformation; Glycogen}},
language = {{eng}},
number = {{4}},
pages = {{458--465}},
publisher = {{Elsevier}},
series = {{International Journal of Biological Macromolecules}},
title = {{Size, structure and scaling relationships in glycogen from various sources investigated with asymmetrical flow field-flow fractionation and 1H NMR}},
url = {{http://dx.doi.org/10.1016/j.ijbiomac.2011.05.016}},
doi = {{10.1016/j.ijbiomac.2011.05.016}},
volume = {{49}},
year = {{2011}},
}