Reduction of ultraviolet light-induced DNA damage in human colon cancer cells treated with a lactoferrin-derived peptide
(2012) In Journal of Dairy Science 95(10). p.5552-5560- Abstract
- Treatment of Caco-2 cells with the peptide lactoferricin(4-14), results in reduction of the growth rate by prolongation of the S phase of the cell cycle. Lactoferricin(1-25) is formed in the gut by cleavage from lactoferrin and the bioactive amino acids are found within lactoferricin(4-14). Our hypothesis is that the reduction of the rate of S phase progression may result in increased DNA repair. To test this hypothesis, Caco-2 cells were subjected to UV light that caused DNA lesions and then the cells were grown in the absence or presence of 2.0 mu M lactoferricin(4-14). Evaluation of DNA strand breaks using the comet assay showed that lactoferricin(4-14) treatment indeed resulted in a reduction of comets showing damaged DNA. In the... (More)
- Treatment of Caco-2 cells with the peptide lactoferricin(4-14), results in reduction of the growth rate by prolongation of the S phase of the cell cycle. Lactoferricin(1-25) is formed in the gut by cleavage from lactoferrin and the bioactive amino acids are found within lactoferricin(4-14). Our hypothesis is that the reduction of the rate of S phase progression may result in increased DNA repair. To test this hypothesis, Caco-2 cells were subjected to UV light that caused DNA lesions and then the cells were grown in the absence or presence of 2.0 mu M lactoferricin(4-14). Evaluation of DNA strand breaks using the comet assay showed that lactoferricin(4-14) treatment indeed resulted in a reduction of comets showing damaged DNA. In the search for a mechanism, we have investigated the levels of several proteins involved in cell cycle regulation, DNA replication, and apoptosis using Western blot. Lactoferricin(4-14) treatment resulted in an increased expression of flap endonuclease-1 pointing to increased DNA synthesis activity. Lactoferricin(4-14) treatment decreased the expression of the proapoptotic protein B-cell lymphoma 2-associated X protein (or Bax), indicating decreased cell death. As we have found previously, lactoferricin(4-14) treatment reduced the expression of cyclin E involved in the G(1)/S transition. Immunofluorescence microscopy showed that a lower gamma-H2AX expression in lactoferricin(4-14)-treated cells, pointing to more efficient DNA repair. Thus, altogether our data show that lactoferricin(4-14) treatment has beneficial effects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3190022
- author
- Freiburghaus, Catja LU ; Lindmark-Mansson, H. ; Paulsson, Marie LU and Oredsson, Stina LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DNA strand break, DNA repair, comet assay, lactoferricin(4-14)
- in
- Journal of Dairy Science
- volume
- 95
- issue
- 10
- pages
- 5552 - 5560
- publisher
- American Dairy Science Association
- external identifiers
-
- wos:000309084600009
- pmid:22901475
- scopus:84867101768
- pmid:22901475
- ISSN
- 1525-3198
- DOI
- 10.3168/jds.2011-5279
- language
- English
- LU publication?
- yes
- id
- d345a297-5c10-4dc7-a6c9-14382e5034e6 (old id 3190022)
- date added to LUP
- 2016-04-01 10:05:54
- date last changed
- 2023-11-09 11:44:02
@article{d345a297-5c10-4dc7-a6c9-14382e5034e6, abstract = {{Treatment of Caco-2 cells with the peptide lactoferricin(4-14), results in reduction of the growth rate by prolongation of the S phase of the cell cycle. Lactoferricin(1-25) is formed in the gut by cleavage from lactoferrin and the bioactive amino acids are found within lactoferricin(4-14). Our hypothesis is that the reduction of the rate of S phase progression may result in increased DNA repair. To test this hypothesis, Caco-2 cells were subjected to UV light that caused DNA lesions and then the cells were grown in the absence or presence of 2.0 mu M lactoferricin(4-14). Evaluation of DNA strand breaks using the comet assay showed that lactoferricin(4-14) treatment indeed resulted in a reduction of comets showing damaged DNA. In the search for a mechanism, we have investigated the levels of several proteins involved in cell cycle regulation, DNA replication, and apoptosis using Western blot. Lactoferricin(4-14) treatment resulted in an increased expression of flap endonuclease-1 pointing to increased DNA synthesis activity. Lactoferricin(4-14) treatment decreased the expression of the proapoptotic protein B-cell lymphoma 2-associated X protein (or Bax), indicating decreased cell death. As we have found previously, lactoferricin(4-14) treatment reduced the expression of cyclin E involved in the G(1)/S transition. Immunofluorescence microscopy showed that a lower gamma-H2AX expression in lactoferricin(4-14)-treated cells, pointing to more efficient DNA repair. Thus, altogether our data show that lactoferricin(4-14) treatment has beneficial effects.}}, author = {{Freiburghaus, Catja and Lindmark-Mansson, H. and Paulsson, Marie and Oredsson, Stina}}, issn = {{1525-3198}}, keywords = {{DNA strand break; DNA repair; comet assay; lactoferricin(4-14)}}, language = {{eng}}, number = {{10}}, pages = {{5552--5560}}, publisher = {{American Dairy Science Association}}, series = {{Journal of Dairy Science}}, title = {{Reduction of ultraviolet light-induced DNA damage in human colon cancer cells treated with a lactoferrin-derived peptide}}, url = {{http://dx.doi.org/10.3168/jds.2011-5279}}, doi = {{10.3168/jds.2011-5279}}, volume = {{95}}, year = {{2012}}, }