Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.
(2013) In Nature Communications 4.- Abstract
- Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without... (More)
- Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3733413
- author
- Åkerblom, Malin LU ; Sachdeva, Rohit LU ; Quintino, Luis LU ; Wettergren, Erika Elgstrand ; Chapman, Katie LU ; Manfre, Giuseppe LU ; Lindvall, Olle LU ; Lundberg, Cecilia LU and Jakobsson, Johan LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 4
- article number
- 1770
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000318872100127
- pmid:23612311
- scopus:84877730401
- pmid:23612311
- ISSN
- 2041-1723
- DOI
- 10.1038/ncomms2801
- language
- English
- LU publication?
- yes
- id
- 7c6f049e-7237-420f-aba8-3c2a582351d7 (old id 3733413)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23612311?dopt=Abstract
- date added to LUP
- 2016-04-01 14:29:34
- date last changed
- 2024-02-25 15:17:45
@article{7c6f049e-7237-420f-aba8-3c2a582351d7, abstract = {{Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.}}, author = {{Åkerblom, Malin and Sachdeva, Rohit and Quintino, Luis and Wettergren, Erika Elgstrand and Chapman, Katie and Manfre, Giuseppe and Lindvall, Olle and Lundberg, Cecilia and Jakobsson, Johan}}, issn = {{2041-1723}}, language = {{eng}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.}}, url = {{http://dx.doi.org/10.1038/ncomms2801}}, doi = {{10.1038/ncomms2801}}, volume = {{4}}, year = {{2013}}, }