Ghrelin and motilin are cosecreted from a prominent endocrine cell population in the small intestine
Wierup, Nils LU ; Björkqvist, Maria LU
; Weström, Björn
LU
; Pierzynowski, Stefan
LU
; Sundler, Frank
LU
and Sjölund, Kristina
LU
(2007)
In Journal of Clinical Endocrinology and Metabolism
92(9).
p.3573-3581
- Abstract
- Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was... (More)
- Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was assessed with immunocytochemistry, in situ hybridization, and immunogold electron microscopy. Plasma ghrelin and motilin levels were measured in pigs, fitted with cannulas in the mesenteric vein. Results: The upper small intestine is unexpectedly rich in ghrelin cells, and these cells contribute to circulating ghrelin. Ghrelin and motilin are coproduced in the same cells in the duodenum and jejunum of both species, and ghrelin and motilin are stored in all secretory granules of such cells in humans, indicating cosecretion. The plasma profiles of ghrelin and motilin in pig were parallel, and a correlation between ghrelin and motilin ( r(2) = 0.22; P < 0.001) was evident in intestinal blood. Conclusions: The upper small intestine is an important source of ghrelin. The likely cosecretion of intestinal ghrelin and motilin suggests concerted actions of the two hormones. These data may have implications for understanding gut motility and clinical implications for dysmotility and bariatric surgery. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/687952
- author
- Wierup, Nils
LU
; Björkqvist, Maria
LU
; Weström, Björn
LU
; Pierzynowski, Stefan
LU
; Sundler, Frank
LU
and Sjölund, Kristina
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ghrelin • motilin • human • porcine • gastro-intestinal tract • gut hormones • co-localisation • co-secretion
- in
- Journal of Clinical Endocrinology and Metabolism
- volume
- 92
- issue
- 9
- pages
- 3573 - 3581
- publisher
- Oxford University Press
- external identifiers
-
- wos:000249279800037
- scopus:34548777165
- pmid:17595255
- ISSN
- 1945-7197
- DOI
- 10.1210/jc.2006-2756
- language
- English
- LU publication?
- yes
- id
- 27d7a12b-a2f3-401d-a7c1-a3bbf07e24d1 (old id 687952)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17595255&dopt=Abstract
- date added to LUP
- 2016-04-01 15:42:25
- date last changed
- 2023-09-04 05:29:33
@article{27d7a12b-a2f3-401d-a7c1-a3bbf07e24d1,
abstract = {{Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was assessed with immunocytochemistry, in situ hybridization, and immunogold electron microscopy. Plasma ghrelin and motilin levels were measured in pigs, fitted with cannulas in the mesenteric vein. Results: The upper small intestine is unexpectedly rich in ghrelin cells, and these cells contribute to circulating ghrelin. Ghrelin and motilin are coproduced in the same cells in the duodenum and jejunum of both species, and ghrelin and motilin are stored in all secretory granules of such cells in humans, indicating cosecretion. The plasma profiles of ghrelin and motilin in pig were parallel, and a correlation between ghrelin and motilin ( r(2) = 0.22; P < 0.001) was evident in intestinal blood. Conclusions: The upper small intestine is an important source of ghrelin. The likely cosecretion of intestinal ghrelin and motilin suggests concerted actions of the two hormones. These data may have implications for understanding gut motility and clinical implications for dysmotility and bariatric surgery.}},
author = {{Wierup, Nils and Björkqvist, Maria and Weström, Björn and Pierzynowski, Stefan and Sundler, Frank and Sjölund, Kristina}},
issn = {{1945-7197}},
keywords = {{ghrelin • motilin • human • porcine • gastro-intestinal tract • gut hormones • co-localisation • co-secretion}},
language = {{eng}},
number = {{9}},
pages = {{3573--3581}},
publisher = {{Oxford University Press}},
series = {{Journal of Clinical Endocrinology and Metabolism}},
title = {{Ghrelin and motilin are cosecreted from a prominent endocrine cell population in the small intestine}},
url = {{http://dx.doi.org/10.1210/jc.2006-2756}},
doi = {{10.1210/jc.2006-2756}},
volume = {{92}},
year = {{2007}},
}