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Structure and interfacial properties of phospholipid-containing sponge nanoparticles and their interaction with myoglobin

Luchini, Alessandra ; Machingauta, Marshall R. LU ; Köhler, Sebastian LU ; Gilbert, Jennifer LU orcid ; Yakimenko, Ivan P. ; Birch, Jens ; Järrendahl, Kenneth ; Cooper, Joshaniel F.K. ; Stendahl, Sjoerd and Langridge, Sean , et al. (2025) In Journal of Colloid and Interface Science 697.
Abstract

Hypothesis: Sponge phase (L3) lipid nanoparticles (L3-NPs) have been shown to have large potential for the encapsulation of biomolecules, such as enzymes, with applications in food and pharmaceutical science. In this study, we introduce new formulations of L3-NPs including the phospholipids dioleoylphosphatidylcholine (DOPC) and dioleoyltrimethylammonium propane (DOTAP). The interaction of these new L3-NPs with myoglobin is of interest for the development of iron supplements which can be incorporated during food processing. Experiments: We characterized the sample structure by small-angle X-ray scattering (SAXS) measurements with and without the addition of myoglobin. We also tested the... (More)

Hypothesis: Sponge phase (L3) lipid nanoparticles (L3-NPs) have been shown to have large potential for the encapsulation of biomolecules, such as enzymes, with applications in food and pharmaceutical science. In this study, we introduce new formulations of L3-NPs including the phospholipids dioleoylphosphatidylcholine (DOPC) and dioleoyltrimethylammonium propane (DOTAP). The interaction of these new L3-NPs with myoglobin is of interest for the development of iron supplements which can be incorporated during food processing. Experiments: We characterized the sample structure by small-angle X-ray scattering (SAXS) measurements with and without the addition of myoglobin. We also tested the myoglobin-lipid interaction in an experimental setup that mimicked the interface between the bilayer and water channels within the bicontinuous sponge structure. This included spreading the L3-NPs onto a hydrophilic surface to form supported lipid bilayers and characterizing their interaction with myoglobin by means of quartz crystal microbalance with dissipation monitoring and polarized neutron reflectometry. Findings: SAXS data indicate that the new formulations containing DOPC and DOTAP formed a sponge phase in the bulk. The data from the surface techniques showed that deposited bilayers containing DOPC were largely unaffected by the addition of myoglobin, whereas those without DOPC were destabilized and partially removed.

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type
Contribution to journal
publication status
published
subject
keywords
Polarised neutron reflectometry, Sponge-phase nanoparticles, Supported lipid bilayers
in
Journal of Colloid and Interface Science
volume
697
article number
137879
publisher
Academic Press
external identifiers
  • scopus:105005874346
  • pmid:40424802
ISSN
0021-9797
DOI
10.1016/j.jcis.2025.137879
language
English
LU publication?
yes
id
6b0b7456-08c8-400c-96ce-92ec92b31259
date added to LUP
2025-07-14 10:21:48
date last changed
2025-07-14 10:22:21
@article{6b0b7456-08c8-400c-96ce-92ec92b31259,
  abstract     = {{<p>Hypothesis: Sponge phase (L<sub>3</sub>) lipid nanoparticles (L<sub>3</sub>-NPs) have been shown to have large potential for the encapsulation of biomolecules, such as enzymes, with applications in food and pharmaceutical science. In this study, we introduce new formulations of L<sub>3</sub>-NPs including the phospholipids dioleoylphosphatidylcholine (DOPC) and dioleoyltrimethylammonium propane (DOTAP). The interaction of these new L<sub>3</sub>-NPs with myoglobin is of interest for the development of iron supplements which can be incorporated during food processing. Experiments: We characterized the sample structure by small-angle X-ray scattering (SAXS) measurements with and without the addition of myoglobin. We also tested the myoglobin-lipid interaction in an experimental setup that mimicked the interface between the bilayer and water channels within the bicontinuous sponge structure. This included spreading the L<sub>3</sub>-NPs onto a hydrophilic surface to form supported lipid bilayers and characterizing their interaction with myoglobin by means of quartz crystal microbalance with dissipation monitoring and polarized neutron reflectometry. Findings: SAXS data indicate that the new formulations containing DOPC and DOTAP formed a sponge phase in the bulk. The data from the surface techniques showed that deposited bilayers containing DOPC were largely unaffected by the addition of myoglobin, whereas those without DOPC were destabilized and partially removed.</p>}},
  author       = {{Luchini, Alessandra and Machingauta, Marshall R. and Köhler, Sebastian and Gilbert, Jennifer and Yakimenko, Ivan P. and Birch, Jens and Järrendahl, Kenneth and Cooper, Joshaniel F.K. and Stendahl, Sjoerd and Langridge, Sean and Kinane, Christy and Caruana, Andrew J. and Dikaia, Olga and Goikhman, Aleksandr and Vorobiev, Alexey and Devishvili, Anton and Hjörvarsson, Björgvin and Nylander, Tommy}},
  issn         = {{0021-9797}},
  keywords     = {{Polarised neutron reflectometry; Sponge-phase nanoparticles; Supported lipid bilayers}},
  language     = {{eng}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Structure and interfacial properties of phospholipid-containing sponge nanoparticles and their interaction with myoglobin}},
  url          = {{http://dx.doi.org/10.1016/j.jcis.2025.137879}},
  doi          = {{10.1016/j.jcis.2025.137879}},
  volume       = {{697}},
  year         = {{2025}},
}