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Exploring vacuum foam drying as an alternative to freeze-drying and spray drying for a human lipase

Osanlóo, Daniel Tristan LU orcid ; Mahlin, Denny ; Bjerregaard, Simon ; Bergenståhl, Björn LU orcid and Millqvist-Fureby, Anna LU (2024) In International Journal of Pharmaceutics 667.
Abstract

This article compares and explores vacuum foam-drying as an alternative drying technology to freeze-drying and spray drying for a recombinant human lipase as the model protein. Materials characteristics such as structure, surface composition and the solid-state properties of the dry materials were compared and investigated. Moreover, the technical functionality in terms of reconstitution characteristics and the lipase stability were also investigated. The stability of the lipase was evaluated through activity measurements. Sucrose and dextran D40 (40 kDa) were used as matrix former and the surfactant α-dodecyl maltoside was used as surface active additive. The study demonstrated that the drying technique greatly influenced the material... (More)

This article compares and explores vacuum foam-drying as an alternative drying technology to freeze-drying and spray drying for a recombinant human lipase as the model protein. Materials characteristics such as structure, surface composition and the solid-state properties of the dry materials were compared and investigated. Moreover, the technical functionality in terms of reconstitution characteristics and the lipase stability were also investigated. The stability of the lipase was evaluated through activity measurements. Sucrose and dextran D40 (40 kDa) were used as matrix former and the surfactant α-dodecyl maltoside was used as surface active additive. The study demonstrated that the drying technique greatly influenced the material structure and composition which in turn affected the reconstitution characteristics. The lipase was overrepresented at the material surface in declining order spray-dried > vacuum foam-dried > freeze-dried. The lipase activity was retained up to 10 % lipase content in solids, but at 20 % lipase a loss of activity was observed for all drying techniques. Phase separation in the solid material may be an explanation. Vacuum foam-drying shows promise as an alternative drying technique for the lipase, and potentially other proteins.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Freeze-drying, Lyophilization, Matrix former, Protein formulations, Spray drying, Surface composition, Vacuum foam-drying
in
International Journal of Pharmaceutics
volume
667
article number
124883
publisher
Elsevier
external identifiers
  • scopus:85207786733
  • pmid:39490556
ISSN
0378-5173
DOI
10.1016/j.ijpharm.2024.124883
language
English
LU publication?
yes
id
9098f007-93b6-4018-9356-867e8104d254
date added to LUP
2024-12-04 10:02:18
date last changed
2025-07-17 04:28:17
@article{9098f007-93b6-4018-9356-867e8104d254,
  abstract     = {{<p>This article compares and explores vacuum foam-drying as an alternative drying technology to freeze-drying and spray drying for a recombinant human lipase as the model protein. Materials characteristics such as structure, surface composition and the solid-state properties of the dry materials were compared and investigated. Moreover, the technical functionality in terms of reconstitution characteristics and the lipase stability were also investigated. The stability of the lipase was evaluated through activity measurements. Sucrose and dextran D40 (40 kDa) were used as matrix former and the surfactant α-dodecyl maltoside was used as surface active additive. The study demonstrated that the drying technique greatly influenced the material structure and composition which in turn affected the reconstitution characteristics. The lipase was overrepresented at the material surface in declining order spray-dried &gt; vacuum foam-dried &gt; freeze-dried. The lipase activity was retained up to 10 % lipase content in solids, but at 20 % lipase a loss of activity was observed for all drying techniques. Phase separation in the solid material may be an explanation. Vacuum foam-drying shows promise as an alternative drying technique for the lipase, and potentially other proteins.</p>}},
  author       = {{Osanlóo, Daniel Tristan and Mahlin, Denny and Bjerregaard, Simon and Bergenståhl, Björn and Millqvist-Fureby, Anna}},
  issn         = {{0378-5173}},
  keywords     = {{Freeze-drying; Lyophilization; Matrix former; Protein formulations; Spray drying; Surface composition; Vacuum foam-drying}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{International Journal of Pharmaceutics}},
  title        = {{Exploring vacuum foam drying as an alternative to freeze-drying and spray drying for a human lipase}},
  url          = {{http://dx.doi.org/10.1016/j.ijpharm.2024.124883}},
  doi          = {{10.1016/j.ijpharm.2024.124883}},
  volume       = {{667}},
  year         = {{2024}},
}