Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection
Papp, Daniel LU ; Rukkijakan, Thanya ; Lebedeva, Daria ; Nylander, Tommy LU ; Sandahl, Margareta LU ; Samec, Joseph S.M. and Turner, Charlotta LU
(2022)
In Analytical Chemistry
95(2).
p.1436-1445
- Abstract
The increased interest in utilizing lignin as a feedstock to produce various aromatic compounds requires advanced chemical analysis methods to provide qualitative and quantitative characterization of lignin samples along different technology streamlines. However, due to the lack of commercially available chemical standards, routine quantification of industrially relevant lignin oligomers in complex lignin samples remains a challenge. This study presents a novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD). A series of lignin-derived dimeric compounds that have been reported from reductive catalytic fractionation (RCF) were synthesized and used as... (More)
The increased interest in utilizing lignin as a feedstock to produce various aromatic compounds requires advanced chemical analysis methods to provide qualitative and quantitative characterization of lignin samples along different technology streamlines. However, due to the lack of commercially available chemical standards, routine quantification of industrially relevant lignin oligomers in complex lignin samples remains a challenge. This study presents a novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD). A series of lignin-derived dimeric compounds that have been reported from reductive catalytic fractionation (RCF) were synthesized and used as standards. The applicability of using linear regression instead of quadratic calibration curves was evaluated over a concentration range of 15-125 mg/L, demonstrating that the former calibration method is as appropriate as the latter. The response factors of lignin dimeric compounds were compared to assess the uniformity of the CAD signal, revealing that the CAD response for the tested lignin dimers did not differ substantially. It was also found that the response factors were not dependent on the number of methoxy groups or linkage motifs, ultimately enabling the use of only one calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed, and the use of a digital peak sharpening technique was adopted and applied to address this challenge. The developed method was verified and used for the quantification of lignin dimers in an oil obtained by a RCF of birch sawdust.
(Less)
- author
- Papp, Daniel
LU
; Rukkijakan, Thanya
; Lebedeva, Daria
; Nylander, Tommy
LU
; Sandahl, Margareta
LU
; Samec, Joseph S.M.
and Turner, Charlotta
LU
- organization
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 95
- issue
- 2
- pages
- 1436 - 1445
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:36548212
- scopus:85144898588
- ISSN
- 0003-2700
- DOI
- 10.1021/acs.analchem.2c04383
- project
- Addressing the challenges of lignin oligomer analysis by liquid-based separation techniques
- language
- English
- LU publication?
- yes
- id
- cee88075-727e-4535-8558-8184a79b4507
- date added to LUP
- 2023-01-23 11:07:36
- date last changed
- 2024-07-09 19:25:15
@article{cee88075-727e-4535-8558-8184a79b4507,
abstract = {{<p>The increased interest in utilizing lignin as a feedstock to produce various aromatic compounds requires advanced chemical analysis methods to provide qualitative and quantitative characterization of lignin samples along different technology streamlines. However, due to the lack of commercially available chemical standards, routine quantification of industrially relevant lignin oligomers in complex lignin samples remains a challenge. This study presents a novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD). A series of lignin-derived dimeric compounds that have been reported from reductive catalytic fractionation (RCF) were synthesized and used as standards. The applicability of using linear regression instead of quadratic calibration curves was evaluated over a concentration range of 15-125 mg/L, demonstrating that the former calibration method is as appropriate as the latter. The response factors of lignin dimeric compounds were compared to assess the uniformity of the CAD signal, revealing that the CAD response for the tested lignin dimers did not differ substantially. It was also found that the response factors were not dependent on the number of methoxy groups or linkage motifs, ultimately enabling the use of only one calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed, and the use of a digital peak sharpening technique was adopted and applied to address this challenge. The developed method was verified and used for the quantification of lignin dimers in an oil obtained by a RCF of birch sawdust.</p>}},
author = {{Papp, Daniel and Rukkijakan, Thanya and Lebedeva, Daria and Nylander, Tommy and Sandahl, Margareta and Samec, Joseph S.M. and Turner, Charlotta}},
issn = {{0003-2700}},
language = {{eng}},
number = {{2}},
pages = {{1436--1445}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Single-Standard Quantification Strategy for Lignin Dimers by Supercritical Fluid Chromatography with Charged Aerosol Detection}},
url = {{http://dx.doi.org/10.1021/acs.analchem.2c04383}},
doi = {{10.1021/acs.analchem.2c04383}},
volume = {{95}},
year = {{2022}},
}