Heparan/chondroitin/dermatan sulfate primer 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside preferentially inhibits growth of transformed cells
(1998) In Cancer Research 58(6). p.104-1099- Abstract
Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally... (More)
Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally growth arrested, whereas normal cells are only < or = 50% inhibited. No inhibition of growth is observed with the stereoisomeric 2-(6-hydroxynaphthyl)-O-beta-L-xylopyranoside, which does not prime glycosaminoglycan synthesis at all; with the nonhydroxylated 2-naphthyl-O-beta-D-xylopyranoside, which only primes CS/DS synthesis under these conditions; or with p-nitrophenyl-O-beta-D-xylopyranoside, which is known to prime only CS/DS synthesis. We conclude that growth inhibition is due to priming of HS and/or CS/DS synthesis, which may either lead to the formation of specific antiproliferative glycans or glycan fragments or to interference with endogenous PG synthesis and turnover.
(Less)
- author
- organization
- publishing date
- 1998-03-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 3T3 Cells, Animals, Chondroitin Sulfates, Dermatan Sulfate, Endothelium, Vascular, Glycosides, Growth Inhibitors, Humans, Mice, Naphthols, Stereoisomerism, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't
- in
- Cancer Research
- volume
- 58
- issue
- 6
- pages
- 6 pages
- publisher
- American Association for Cancer Research Inc.
- external identifiers
-
- pmid:9515787
- scopus:0032521158
- ISSN
- 0008-5472
- language
- English
- LU publication?
- yes
- id
- d87f239a-f6b2-4566-9d01-fa6f333b23ba
- date added to LUP
- 2017-06-27 14:13:47
- date last changed
- 2024-08-05 00:01:55
@article{d87f239a-f6b2-4566-9d01-fa6f333b23ba, abstract = {{<p>Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally growth arrested, whereas normal cells are only < or = 50% inhibited. No inhibition of growth is observed with the stereoisomeric 2-(6-hydroxynaphthyl)-O-beta-L-xylopyranoside, which does not prime glycosaminoglycan synthesis at all; with the nonhydroxylated 2-naphthyl-O-beta-D-xylopyranoside, which only primes CS/DS synthesis under these conditions; or with p-nitrophenyl-O-beta-D-xylopyranoside, which is known to prime only CS/DS synthesis. We conclude that growth inhibition is due to priming of HS and/or CS/DS synthesis, which may either lead to the formation of specific antiproliferative glycans or glycan fragments or to interference with endogenous PG synthesis and turnover.</p>}}, author = {{Mani, K and Havsmark, B and Persson, Susanne and Kaneda, Y and Yamamoto, H and Sakurai, K and Ashikari, S and Habuchi, H and Suzuki, S and Kimata, K and Malmström, A and Westergren-Thorsson, G and Fransson, L A}}, issn = {{0008-5472}}, keywords = {{3T3 Cells; Animals; Chondroitin Sulfates; Dermatan Sulfate; Endothelium, Vascular; Glycosides; Growth Inhibitors; Humans; Mice; Naphthols; Stereoisomerism; Tumor Cells, Cultured; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, month = {{03}}, number = {{6}}, pages = {{104--1099}}, publisher = {{American Association for Cancer Research Inc.}}, series = {{Cancer Research}}, title = {{Heparan/chondroitin/dermatan sulfate primer 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside preferentially inhibits growth of transformed cells}}, volume = {{58}}, year = {{1998}}, }