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Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosis

Kadefors, Måns LU ; Berlin, Frida LU ; Wildt, Marie LU ; Dellgren, Göran ; Rolandsson Enes, Sara LU orcid ; Aspberg, Anders LU orcid and Westergren-Thorsson, Gunilla LU orcid (2022) In Frontiers in Pharmacology 13. p.1-12
Abstract

Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4
+ fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined
in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4
+ fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4
+ fibroblasts in pathohistological features... (More)

Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4
+ fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined
in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4
+ fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4
+ fibroblasts in pathohistological features of IPF. The
in vivo observations were supported by results
in vitro showing a decreased expression of DPP4 on normal and IPF fibroblasts after profibrotic stimuli (transforming growth factor β) and no effect on the expression of activation markers (α-smooth muscle actin, collagen I and connective tissue growth factor) upon knockout of DPP4 in lung fibroblasts with or without activation with profibrotic stimuli.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Pharmacology
volume
13
article number
953771
pages
1 - 12
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85137973924
  • pmid:36120350
ISSN
1663-9812
DOI
10.3389/fphar.2022.953771
project
Characterization of lung-derived mesenchymal stromal cells
language
English
LU publication?
yes
additional info
Copyright © 2022 Kadefors, Berlin, Wildt, Dellgren, Rolandsson Enes, Aspberg and Westergren-Thorsson.
id
d9371285-3ac6-44c8-a078-40b4c9565973
date added to LUP
2022-09-22 17:20:39
date last changed
2024-07-09 21:12:40
@article{d9371285-3ac6-44c8-a078-40b4c9565973,
  abstract     = {{<p>Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4<br>
 + fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined <br>
 in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4<br>
 + fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4<br>
 + fibroblasts in pathohistological features of IPF. The <br>
 in vivo observations were supported by results <br>
 in vitro showing a decreased expression of DPP4 on normal and IPF fibroblasts after profibrotic stimuli (transforming growth factor β) and no effect on the expression of activation markers (α-smooth muscle actin, collagen I and connective tissue growth factor) upon knockout of DPP4 in lung fibroblasts with or without activation with profibrotic stimuli.<br>
 </p>}},
  author       = {{Kadefors, Måns and Berlin, Frida and Wildt, Marie and Dellgren, Göran and Rolandsson Enes, Sara and Aspberg, Anders and Westergren-Thorsson, Gunilla}},
  issn         = {{1663-9812}},
  language     = {{eng}},
  pages        = {{1--12}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Pharmacology}},
  title        = {{Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosis}},
  url          = {{http://dx.doi.org/10.3389/fphar.2022.953771}},
  doi          = {{10.3389/fphar.2022.953771}},
  volume       = {{13}},
  year         = {{2022}},
}