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Expression of Stress-Induced Genes in Bronchoalveolar Lavage Cells and Lung Fibroblasts from Healthy and COPD Subjects

Garcia-Ryde, Martin LU ; van der Burg, Nicole M D LU orcid ; Berlin, Frida LU ; Westergren-Thorsson, Gunilla LU orcid ; Bjermer, Leif LU ; Ankerst, Jaro LU orcid ; Larsson-Callerfelt, Anna-Karin LU orcid ; Andersson, Cecilia K LU and Tufvesson, Ellen LU (2024) In International Journal of Molecular Sciences 25(12). p.1-16
Abstract

Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene... (More)

Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene expression of CHOP in lung fibroblasts from COPD subjects. Exposure to CSE caused inhibition of lung fibroblast proliferation in both groups, though the changes in ER stress-related gene expressions (ATF6, IRE1, PERK, ATF4, CHOP, BCL2L1) and genes relating to proteasomal subunits mostly occurred in healthy lung fibroblasts. No differences were found in BAL cells. In this study, we have found that lung fibroblasts from COPD subjects have an atypical ER stress gene response to CSE, particularly in genes related to apoptosis. This difference in response to CSE may be a contributing factor to COPD progression.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Humans, Pulmonary Disease, Chronic Obstructive/genetics, Fibroblasts/metabolism, Endoplasmic Reticulum Stress/genetics, Male, Female, Middle Aged, Lung/metabolism, Bronchoalveolar Lavage Fluid/cytology, Aged, Cell Proliferation, Gene Expression Regulation, Cells, Cultured, Apoptosis/genetics, Case-Control Studies
in
International Journal of Molecular Sciences
volume
25
issue
12
article number
6600
pages
1 - 16
publisher
MDPI AG
external identifiers
  • pmid:38928305
ISSN
1422-0067
DOI
10.3390/ijms25126600
language
English
LU publication?
yes
id
f7d0b4fa-7136-452a-99bd-9bd6f232b6ec
date added to LUP
2024-06-30 15:02:15
date last changed
2024-07-01 08:30:00
@article{f7d0b4fa-7136-452a-99bd-9bd6f232b6ec,
  abstract     = {{<p>Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene expression of CHOP in lung fibroblasts from COPD subjects. Exposure to CSE caused inhibition of lung fibroblast proliferation in both groups, though the changes in ER stress-related gene expressions (ATF6, IRE1, PERK, ATF4, CHOP, BCL2L1) and genes relating to proteasomal subunits mostly occurred in healthy lung fibroblasts. No differences were found in BAL cells. In this study, we have found that lung fibroblasts from COPD subjects have an atypical ER stress gene response to CSE, particularly in genes related to apoptosis. This difference in response to CSE may be a contributing factor to COPD progression.</p>}},
  author       = {{Garcia-Ryde, Martin and van der Burg, Nicole M D and Berlin, Frida and Westergren-Thorsson, Gunilla and Bjermer, Leif and Ankerst, Jaro and Larsson-Callerfelt, Anna-Karin and Andersson, Cecilia K and Tufvesson, Ellen}},
  issn         = {{1422-0067}},
  keywords     = {{Humans; Pulmonary Disease, Chronic Obstructive/genetics; Fibroblasts/metabolism; Endoplasmic Reticulum Stress/genetics; Male; Female; Middle Aged; Lung/metabolism; Bronchoalveolar Lavage Fluid/cytology; Aged; Cell Proliferation; Gene Expression Regulation; Cells, Cultured; Apoptosis/genetics; Case-Control Studies}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{12}},
  pages        = {{1--16}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Expression of Stress-Induced Genes in Bronchoalveolar Lavage Cells and Lung Fibroblasts from Healthy and COPD Subjects}},
  url          = {{http://dx.doi.org/10.3390/ijms25126600}},
  doi          = {{10.3390/ijms25126600}},
  volume       = {{25}},
  year         = {{2024}},
}