Expression of Stress-Induced Genes in Bronchoalveolar Lavage Cells and Lung Fibroblasts from Healthy and COPD Subjects
(2024) In International Journal of Molecular Sciences 25(12). p.1-16- Abstract
Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene... (More)
Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene expression of CHOP in lung fibroblasts from COPD subjects. Exposure to CSE caused inhibition of lung fibroblast proliferation in both groups, though the changes in ER stress-related gene expressions (ATF6, IRE1, PERK, ATF4, CHOP, BCL2L1) and genes relating to proteasomal subunits mostly occurred in healthy lung fibroblasts. No differences were found in BAL cells. In this study, we have found that lung fibroblasts from COPD subjects have an atypical ER stress gene response to CSE, particularly in genes related to apoptosis. This difference in response to CSE may be a contributing factor to COPD progression.
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- author
- Garcia-Ryde, Martin LU ; van der Burg, Nicole M D LU ; Berlin, Frida LU ; Westergren-Thorsson, Gunilla LU ; Bjermer, Leif LU ; Ankerst, Jaro LU ; Larsson-Callerfelt, Anna-Karin LU ; Andersson, Cecilia K LU and Tufvesson, Ellen LU
- organization
-
- Lung Biology (research group)
- Lung physiology and biomarkers (research group)
- Respiratory Cell Biology (research group)
- WCMM-Wallenberg Centre for Molecular Medicine
- eSSENCE: The e-Science Collaboration
- Lund University Bioimaging Center
- Clinical Respiratory Medicine (research group)
- EpiHealth: Epidemiology for Health
- LTH Profile Area: Aerosols
- Paediatric Endocrinology (research group)
- EXODIAB: Excellence of Diabetes Research in Sweden
- Tornblad Institute (research group)
- Neonatology (research group)
- publishing date
- 2024-06-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Humans, Pulmonary Disease, Chronic Obstructive/genetics, Fibroblasts/metabolism, Endoplasmic Reticulum Stress/genetics, Male, Female, Middle Aged, Lung/metabolism, Bronchoalveolar Lavage Fluid/cytology, Aged, Cell Proliferation, Gene Expression Regulation, Cells, Cultured, Apoptosis/genetics, Case-Control Studies
- in
- International Journal of Molecular Sciences
- volume
- 25
- issue
- 12
- article number
- 6600
- pages
- 1 - 16
- publisher
- MDPI AG
- external identifiers
-
- pmid:38928305
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms25126600
- language
- English
- LU publication?
- yes
- id
- f7d0b4fa-7136-452a-99bd-9bd6f232b6ec
- date added to LUP
- 2024-06-30 15:02:15
- date last changed
- 2024-07-01 08:30:00
@article{f7d0b4fa-7136-452a-99bd-9bd6f232b6ec, abstract = {{<p>Chronic obstructive pulmonary disease (COPD) is commonly caused from smoking cigarettes that induce biological stress responses. Previously we found disorganized endoplasmic reticulum (ER) in fibroblasts from COPD with different responses to chemical stressors compared to healthy subjects. Here, we aimed to investigate differences in stress-related gene expressions within lung cells from COPD and healthy subjects. Bronchoalveolar lavage (BAL) cells were collected from seven COPD and 35 healthy subjects. Lung fibroblasts were derived from 19 COPD and 24 healthy subjects and exposed to cigarette smoke extract (CSE). Gene and protein expression and cell proliferation were investigated. Compared to healthy subjects, we found lower gene expression of CHOP in lung fibroblasts from COPD subjects. Exposure to CSE caused inhibition of lung fibroblast proliferation in both groups, though the changes in ER stress-related gene expressions (ATF6, IRE1, PERK, ATF4, CHOP, BCL2L1) and genes relating to proteasomal subunits mostly occurred in healthy lung fibroblasts. No differences were found in BAL cells. In this study, we have found that lung fibroblasts from COPD subjects have an atypical ER stress gene response to CSE, particularly in genes related to apoptosis. This difference in response to CSE may be a contributing factor to COPD progression.</p>}}, author = {{Garcia-Ryde, Martin and van der Burg, Nicole M D and Berlin, Frida and Westergren-Thorsson, Gunilla and Bjermer, Leif and Ankerst, Jaro and Larsson-Callerfelt, Anna-Karin and Andersson, Cecilia K and Tufvesson, Ellen}}, issn = {{1422-0067}}, keywords = {{Humans; Pulmonary Disease, Chronic Obstructive/genetics; Fibroblasts/metabolism; Endoplasmic Reticulum Stress/genetics; Male; Female; Middle Aged; Lung/metabolism; Bronchoalveolar Lavage Fluid/cytology; Aged; Cell Proliferation; Gene Expression Regulation; Cells, Cultured; Apoptosis/genetics; Case-Control Studies}}, language = {{eng}}, month = {{06}}, number = {{12}}, pages = {{1--16}}, publisher = {{MDPI AG}}, series = {{International Journal of Molecular Sciences}}, title = {{Expression of Stress-Induced Genes in Bronchoalveolar Lavage Cells and Lung Fibroblasts from Healthy and COPD Subjects}}, url = {{http://dx.doi.org/10.3390/ijms25126600}}, doi = {{10.3390/ijms25126600}}, volume = {{25}}, year = {{2024}}, }