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Cultivation of the gut bacterium Prevotella copri DSM 18205T using glucose and xylose as carbon sources

Huang, Fang LU ; Sardari, Roya R R LU ; Jasilionis, Andrius LU ; Böök, Olof ; Öste, Rickard LU ; Rascón, Ana LU ; Heyman-Lindén, Lovisa ; Holst, Olle LU and Karlsson, Eva Nordberg LU orcid (2021) In MicrobiologyOpen 10(3).
Abstract

Prevotella copri DSM18205T is a human gut bacterium, suggested as a next-generation probiotic. To utilize it as such, it is, however, necessary to grow the species in a reproducible manner. Prevotella copri has previously been reported to be highly sensitive to oxygen, and hence difficult to isolate and cultivate. This study presents successful batch cultivation strategies for viable strain inoculations and growth in both serum bottles and a stirred tank bioreactor (STR), without the use of an anaerobic chamber, as long as the cells were kept in the exponential growth phase. A low headspace volume in the STR was important to reach high cell density. P. copri utilized xylose cultivated in Peptone Yeast Xylose medium (PYX medium),... (More)

Prevotella copri DSM18205T is a human gut bacterium, suggested as a next-generation probiotic. To utilize it as such, it is, however, necessary to grow the species in a reproducible manner. Prevotella copri has previously been reported to be highly sensitive to oxygen, and hence difficult to isolate and cultivate. This study presents successful batch cultivation strategies for viable strain inoculations and growth in both serum bottles and a stirred tank bioreactor (STR), without the use of an anaerobic chamber, as long as the cells were kept in the exponential growth phase. A low headspace volume in the STR was important to reach high cell density. P. copri utilized xylose cultivated in Peptone Yeast Xylose medium (PYX medium), resulting in a comparable growth rate and metabolite production as in Peptone Yeast Glucose medium (PYG medium) in batch cultivations at pH 7.2.Up to 5 g/L of the carbon source was consumed, leading to the production of succinic acid, acetic acid, and formic acid, and cell densities (OD620 nm ) in the range 6-7.5. The highest yield of produced succinic acid was 0.63 ± 0.05 g/g glucose in PYG medium cultivations and 0.88 ± 0.06 g/g xylose in PYX medium cultivations.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anaerobe, bioreactor, cultivaton, fermentation, prevotella copri, type strain
in
MicrobiologyOpen
volume
10
issue
3
article number
e1213
pages
15 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85108958200
  • pmid:34180602
ISSN
2045-8827
DOI
10.1002/mbo3.1213
language
English
LU publication?
yes
id
04c832db-ca5a-460d-9387-14f5989dac55
date added to LUP
2021-07-01 20:31:12
date last changed
2025-03-10 18:22:22
@article{04c832db-ca5a-460d-9387-14f5989dac55,
  abstract     = {{<p>Prevotella copri DSM18205T is a human gut bacterium, suggested as a next-generation probiotic. To utilize it as such, it is, however, necessary to grow the species in a reproducible manner. Prevotella copri has previously been reported to be highly sensitive to oxygen, and hence difficult to isolate and cultivate. This study presents successful batch cultivation strategies for viable strain inoculations and growth in both serum bottles and a stirred tank bioreactor (STR), without the use of an anaerobic chamber, as long as the cells were kept in the exponential growth phase. A low headspace volume in the STR was important to reach high cell density. P. copri utilized xylose cultivated in Peptone Yeast Xylose medium (PYX medium), resulting in a comparable growth rate and metabolite production as in Peptone Yeast Glucose medium (PYG medium) in batch cultivations at pH 7.2.Up to 5 g/L of the carbon source was consumed, leading to the production of succinic acid, acetic acid, and formic acid, and cell densities (OD620 nm ) in the range 6-7.5. The highest yield of produced succinic acid was 0.63 ± 0.05 g/g glucose in PYG medium cultivations and 0.88 ± 0.06 g/g xylose in PYX medium cultivations.</p>}},
  author       = {{Huang, Fang and Sardari, Roya R R and Jasilionis, Andrius and Böök, Olof and Öste, Rickard and Rascón, Ana and Heyman-Lindén, Lovisa and Holst, Olle and Karlsson, Eva Nordberg}},
  issn         = {{2045-8827}},
  keywords     = {{Anaerobe; bioreactor; cultivaton; fermentation; prevotella copri; type strain}},
  language     = {{eng}},
  number       = {{3}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{MicrobiologyOpen}},
  title        = {{Cultivation of the gut bacterium Prevotella copri DSM 18205T using glucose and xylose as carbon sources}},
  url          = {{http://dx.doi.org/10.1002/mbo3.1213}},
  doi          = {{10.1002/mbo3.1213}},
  volume       = {{10}},
  year         = {{2021}},
}