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Nano-Infrared Imaging of Primary Neurons

O. Freitas, Raul ; Cernescu, Adrian ; Engdahl, Anders LU ; Paulus, Agnes LU ; E. Levandoski, João ; Martinsson, Isak LU ; Hebisch, Elke LU ; Sandt, Christophe ; Keppler Gouras, Gunnar LU orcid and Prinz, Christelle LU , et al. (2021) In Cells 10(10). p.1-15
Abstract
Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM)... (More)
Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid β-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect Aβ-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists’ awareness of the potential of s-SNOM as a tool for analyzing amyloid β-sheet structures at the nanoscale in neurons without the need for immunolabeling (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
O-PTIR, s-SNOM, Alzheimer’s disease, neuron, amyloid-beta
in
Cells
volume
10
issue
10
article number
2559
pages
1 - 15
publisher
MDPI AG
external identifiers
  • scopus:85115787740
  • pmid:34685539
ISSN
2073-4409
DOI
10.3390/cells10102559
language
English
LU publication?
yes
id
0dab6a6b-e62e-44a0-9586-ac14066cc3b9
date added to LUP
2021-09-28 23:13:38
date last changed
2023-11-08 19:59:33
@article{0dab6a6b-e62e-44a0-9586-ac14066cc3b9,
  abstract     = {{Alzheimer’s disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Numerous intensive in vitro studies have provided detailed information about amyloid polymorphs; however, little is known on how amyloid β-sheet-enriched aggregates can cause neurotoxicity in relevant settings. We used scattering-type scanning near-field optical microscopy (s-SNOM) to study amyloid structures at the nanoscale, in individual neurons. Specifically, we show that in well-validated systems, s-SNOM can detect amyloid β-sheet structures with nanometer spatial resolution in individual neurons. This is a proof-of-concept study to demonstrate that s-SNOM can be used to detect Aβ-sheet structures on cell surfaces at the nanoscale. Furthermore, this study is intended to raise neurobiologists’ awareness of the potential of s-SNOM as a tool for analyzing amyloid β-sheet structures at the nanoscale in neurons without the need for immunolabeling}},
  author       = {{O. Freitas, Raul and Cernescu, Adrian and Engdahl, Anders and Paulus, Agnes and E. Levandoski, João and Martinsson, Isak and Hebisch, Elke and Sandt, Christophe and Keppler Gouras, Gunnar and Prinz, Christelle and Deierborg, Tomas and Borondics, Ferenc and Klementieva, Oxana}},
  issn         = {{2073-4409}},
  keywords     = {{O-PTIR; s-SNOM; Alzheimer’s disease; neuron; amyloid-beta}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1--15}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{Nano-Infrared Imaging of Primary Neurons}},
  url          = {{http://dx.doi.org/10.3390/cells10102559}},
  doi          = {{10.3390/cells10102559}},
  volume       = {{10}},
  year         = {{2021}},
}