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The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.

Gjörloff Wingren, Anette LU ; Hadzic, Radinka LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2002) In Journal of Immunology 168(11). p.5582-5588
Abstract
Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition... (More)
Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells. (Less)
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keywords
Immunoglobulin D : physiology, Immunoglobulin A : biosynthesis, Human, Drug, Dose-Response Relationship, Cytokines, Carrier Proteins : pharmacology, B-Lymphocytes : immunology, B-Lymphocytes : drug effects, Interleukin-6 : biosynthesis, Lymphocyte Transformation : drug effects, Support, Non-U.S. Gov't, Th2 Cells : immunology, Immunoglobulin G : biosynthesis, Immunoglobulin M : biosynthesis, Interleukin-2 : pharmacology
in
Journal of Immunology
volume
168
issue
11
pages
5582 - 5588
publisher
American Association of Immunologists
external identifiers
  • wos:000175815200026
  • pmid:12023354
  • scopus:0036604206
ISSN
1550-6606
language
English
LU publication?
yes
id
0e476d19-7e97-4ec9-91a2-562bcf1b93e2 (old id 108357)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12023354&dopt=Abstract
date added to LUP
2007-07-25 09:21:54
date last changed
2017-12-10 04:33:58
@article{0e476d19-7e97-4ec9-91a2-562bcf1b93e2,
  abstract     = {Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells.},
  author       = {Gjörloff Wingren, Anette and Hadzic, Radinka and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1550-6606},
  keyword      = {Immunoglobulin D : physiology,Immunoglobulin A : biosynthesis,Human,Drug,Dose-Response Relationship,Cytokines,Carrier Proteins : pharmacology,B-Lymphocytes : immunology,B-Lymphocytes : drug effects,Interleukin-6 : biosynthesis,Lymphocyte Transformation : drug effects,Support,Non-U.S. Gov't,Th2 Cells : immunology,Immunoglobulin G : biosynthesis,Immunoglobulin M : biosynthesis,Interleukin-2 : pharmacology},
  language     = {eng},
  number       = {11},
  pages        = {5582--5588},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.},
  volume       = {168},
  year         = {2002},
}