Expression of somatostatin receptor subtypes 2 and 4 in human benign prostatic hyperplasia and prostatic cancer.
(2002) In The Prostate 53(1). p.50-59- Abstract
- BACKGROUND: The presence of receptor subtypes for the inhibitory peptide somatostatin in prostatic tissue has been a controversial issue with conflicting reports. To elucidate whether prostatic epithelial cells express mRNA for somatostatin receptor (SSTR) subtype 2 and 4, we have investigated the localization of SSTR2 and SSTR4 transcripts in prostatic tissues by in situ hybridization. METHODS: Nonradioactive in situ hybridization was performed with specific fluorescein-labeled SSTR2 and SSTR4 riboprobes on consecutive sections of benign prostatic hyperplasia (BPH) and prostate cancer tissues. RESULTS: We report, for the first time, tissue localization of SSTR2 and SSTR4 mRNA in BPH and malignant cells of human prostate. Hybridization... (More)
- BACKGROUND: The presence of receptor subtypes for the inhibitory peptide somatostatin in prostatic tissue has been a controversial issue with conflicting reports. To elucidate whether prostatic epithelial cells express mRNA for somatostatin receptor (SSTR) subtype 2 and 4, we have investigated the localization of SSTR2 and SSTR4 transcripts in prostatic tissues by in situ hybridization. METHODS: Nonradioactive in situ hybridization was performed with specific fluorescein-labeled SSTR2 and SSTR4 riboprobes on consecutive sections of benign prostatic hyperplasia (BPH) and prostate cancer tissues. RESULTS: We report, for the first time, tissue localization of SSTR2 and SSTR4 mRNA in BPH and malignant cells of human prostate. Hybridization signals for SSTR4 mRNA transcripts were confined to the prostatic epithelium (12 of 16 BPH cases, and in 12 of 13 carcinoma cases), whereas SSTR2 transcripts were predominantly localized in the stromal compartment but also were detectable in epithelial cells in a significant number of specimens (11 of 17 BPH cases, and in 12 of 14 carcinoma cases). Furthermore, the staining intensity for SSTR2 and SSTR4 transcripts is stronger in malignant cells compared with adjacent BPH epithelium. CONCLUSION: The data presented suggest that the expression of SSTR2 and SSTR4 transcripts is up-regulated in malignant cells and that not only SSTR2 agonists, but also compounds targeting the SSTR4 subtype may have a potential role in the treatment of prostate cancer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/110372
- author
- Ceder, Jens LU ; Bjartell, Anders LU ; Gadaleanu, Virgil ; Dizeyi, Nishtman LU and Abrahamsson, Per-Anders LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gene Expression Regulation, Human, In Situ Hybridization, Male, DNA Primers, Carcinoma: pathology, RNA, Messenger: analysis, Prostatic Hyperplasia: pathology, Prostatic Neoplasms: pathology, Messenger: biosynthesis, Receptors, Somatostatin: biosynthesis, Support, Non-U.S. Gov't, Up-Regulation
- in
- The Prostate
- volume
- 53
- issue
- 1
- pages
- 50 - 59
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:12210479
- wos:000177756000005
- scopus:0037106043
- pmid:12210479
- ISSN
- 0270-4137
- DOI
- 10.1002/pros.10121
- language
- English
- LU publication?
- yes
- id
- 0bab57ac-8d2d-437d-b50b-564b0e380db4 (old id 110372)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12210479&dopt=Abstract
- date added to LUP
- 2016-04-04 08:54:39
- date last changed
- 2022-01-29 07:34:18
@article{0bab57ac-8d2d-437d-b50b-564b0e380db4, abstract = {{BACKGROUND: The presence of receptor subtypes for the inhibitory peptide somatostatin in prostatic tissue has been a controversial issue with conflicting reports. To elucidate whether prostatic epithelial cells express mRNA for somatostatin receptor (SSTR) subtype 2 and 4, we have investigated the localization of SSTR2 and SSTR4 transcripts in prostatic tissues by in situ hybridization. METHODS: Nonradioactive in situ hybridization was performed with specific fluorescein-labeled SSTR2 and SSTR4 riboprobes on consecutive sections of benign prostatic hyperplasia (BPH) and prostate cancer tissues. RESULTS: We report, for the first time, tissue localization of SSTR2 and SSTR4 mRNA in BPH and malignant cells of human prostate. Hybridization signals for SSTR4 mRNA transcripts were confined to the prostatic epithelium (12 of 16 BPH cases, and in 12 of 13 carcinoma cases), whereas SSTR2 transcripts were predominantly localized in the stromal compartment but also were detectable in epithelial cells in a significant number of specimens (11 of 17 BPH cases, and in 12 of 14 carcinoma cases). Furthermore, the staining intensity for SSTR2 and SSTR4 transcripts is stronger in malignant cells compared with adjacent BPH epithelium. CONCLUSION: The data presented suggest that the expression of SSTR2 and SSTR4 transcripts is up-regulated in malignant cells and that not only SSTR2 agonists, but also compounds targeting the SSTR4 subtype may have a potential role in the treatment of prostate cancer.}}, author = {{Ceder, Jens and Bjartell, Anders and Gadaleanu, Virgil and Dizeyi, Nishtman and Abrahamsson, Per-Anders}}, issn = {{0270-4137}}, keywords = {{Gene Expression Regulation; Human; In Situ Hybridization; Male; DNA Primers; Carcinoma: pathology; RNA; Messenger: analysis; Prostatic Hyperplasia: pathology; Prostatic Neoplasms: pathology; Messenger: biosynthesis; Receptors; Somatostatin: biosynthesis; Support; Non-U.S. Gov't; Up-Regulation}}, language = {{eng}}, number = {{1}}, pages = {{50--59}}, publisher = {{John Wiley & Sons Inc.}}, series = {{The Prostate}}, title = {{Expression of somatostatin receptor subtypes 2 and 4 in human benign prostatic hyperplasia and prostatic cancer.}}, url = {{http://dx.doi.org/10.1002/pros.10121}}, doi = {{10.1002/pros.10121}}, volume = {{53}}, year = {{2002}}, }