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Rat sciatic nerve regeneration through a micromachined silicon chip

Zhao, Qing; Drott, Johan; Laurell, Thomas LU ; Wallman, Lars LU ; Lindström, Kjell LU ; Bjursten, Lars Magnus LU ; Lundborg, Göran LU ; Montelius, Lars LU and Danielsen, Nils LU (1997) In Biomaterials 18(1). p.75-80
Abstract
The capacity of regenerating nerve fibres to grow through a perforated silicon chip was tested using the silicone chamber model for nerve regeneration. The chips were fabricated as circular membranes, 4 mm in diameter, thickness 60 microns, with a perforated area, 2 mm in diameter, in the centre. Three types of chips were fabricated utilizing anisotropic etching. The chips were glued with silicone adhesive between two halves of silicone rubber tubing (total length 8 mm, inner diameter 1.8 mm, outer diameter 3.0 mm) which was used to bridge a 4 mm gap between the proximal and distal nerve stumps of a transected rat sciatic nerve. The capacity of regenerating nerve fibres to grow through the holes of the chip was analysed by light and... (More)
The capacity of regenerating nerve fibres to grow through a perforated silicon chip was tested using the silicone chamber model for nerve regeneration. The chips were fabricated as circular membranes, 4 mm in diameter, thickness 60 microns, with a perforated area, 2 mm in diameter, in the centre. Three types of chips were fabricated utilizing anisotropic etching. The chips were glued with silicone adhesive between two halves of silicone rubber tubing (total length 8 mm, inner diameter 1.8 mm, outer diameter 3.0 mm) which was used to bridge a 4 mm gap between the proximal and distal nerve stumps of a transected rat sciatic nerve. The capacity of regenerating nerve fibres to grow through the holes of the chip was analysed by light and scanning electron microscopy after 4 or 16 weeks of regeneration. Furthermore, the muscle contractility force of the gastrocnemius muscle was measured after 16 weeks of regeneration and compared as a percentage of the contralateral uninjured side. Nerves generated through chips with hole diameters of 10 or 50 microns were morphological and functional failures. The nerve structures distal to chips with hole diameters of 100 microns contained many myelinated nerve fibres in a minifascicular pattern after both 4 and 16 weeks of regeneration. The muscle contractility force was 56% of that of contralateral control muscles. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Silicon chip, nerve regeneration, rat sciatic nerve, sieve electrode
in
Biomaterials
volume
18
issue
1
pages
75 - 80
publisher
Elsevier
external identifiers
  • pmid:9003901
  • scopus:0031032712
ISSN
1878-5905
DOI
10.1016/S0142-9612(96)00112-3
language
English
LU publication?
yes
id
8fb32092-558f-484a-b4e3-1975a3289d3d (old id 1111344)
date added to LUP
2008-07-17 11:39:50
date last changed
2017-01-29 03:29:13
@article{8fb32092-558f-484a-b4e3-1975a3289d3d,
  abstract     = {The capacity of regenerating nerve fibres to grow through a perforated silicon chip was tested using the silicone chamber model for nerve regeneration. The chips were fabricated as circular membranes, 4 mm in diameter, thickness 60 microns, with a perforated area, 2 mm in diameter, in the centre. Three types of chips were fabricated utilizing anisotropic etching. The chips were glued with silicone adhesive between two halves of silicone rubber tubing (total length 8 mm, inner diameter 1.8 mm, outer diameter 3.0 mm) which was used to bridge a 4 mm gap between the proximal and distal nerve stumps of a transected rat sciatic nerve. The capacity of regenerating nerve fibres to grow through the holes of the chip was analysed by light and scanning electron microscopy after 4 or 16 weeks of regeneration. Furthermore, the muscle contractility force of the gastrocnemius muscle was measured after 16 weeks of regeneration and compared as a percentage of the contralateral uninjured side. Nerves generated through chips with hole diameters of 10 or 50 microns were morphological and functional failures. The nerve structures distal to chips with hole diameters of 100 microns contained many myelinated nerve fibres in a minifascicular pattern after both 4 and 16 weeks of regeneration. The muscle contractility force was 56% of that of contralateral control muscles.},
  author       = {Zhao, Qing and Drott, Johan and Laurell, Thomas and Wallman, Lars and Lindström, Kjell and Bjursten, Lars Magnus and Lundborg, Göran and Montelius, Lars and Danielsen, Nils},
  issn         = {1878-5905},
  keyword      = {Silicon chip,nerve regeneration,rat sciatic nerve,sieve electrode},
  language     = {eng},
  number       = {1},
  pages        = {75--80},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Rat sciatic nerve regeneration through a micromachined silicon chip},
  url          = {http://dx.doi.org/10.1016/S0142-9612(96)00112-3},
  volume       = {18},
  year         = {1997},
}