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Nerve regeneration in a 'pseudo-nerve' graft created in a silicone tube

Zhao, Q; Lundborg, Göran LU ; Danielsen, Nils LU ; Bjursten, Lars Magnus LU and Dahlin, Lars LU (1997) In Brain Research1966-01-01+01:00 769(1). p.125-134
Abstract
The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament... (More)
The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament staining after 6 days or by morphology after 4, 6 or 8 weeks. The results showed that the pseudo-nerve could induce nerve regeneration to a similar extend as a real nerve graft. The neurobiological composition of the pseudo-nerve and the factors influencing its formation were also studied. By double staining of S-100 and laminin we found that the longitudinally organized Schwann cell columns in the pseudo-nerve were surrounded by basal laminae and ensheathed by a layer of vascularized perineurium-like tissue. Macrophages (ED1 and ED2) and their products interleukin-1beta (IL-1beta) and transforming growth factor-beta1 (TGF-beta1) were constantly present in the pseudo-nerve. Besides, the size of tube was a crucial factor in influencing pseudo-nerve formation, e.g. a thicker pseudo-nerve was formed in tubes with larger diameters or shorter gap lengths. No pseudo-nerve was formed when the gap was 15 mm long. When both proximal and distal inserts were isolated nerve segments the pseudo-nerve was still formed but thin, probably because of compromised vascular supply. Taken together, the results suggested that the pseudo-nerve contains the essential neurobiological elements to induce successful axonal elongation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nerve regeneration, Peripheral nerve, Nerve graft, Schwann cell, Silicone, Implant, Matrix, Immunohistochemistry
in
Brain Research1966-01-01+01:00
volume
769
issue
1
pages
125 - 134
publisher
Elsevier
external identifiers
  • pmid:9374280
  • scopus:0030691095
ISSN
1872-6240
DOI
10.1016/S0006-8993(97)00620-3
language
English
LU publication?
yes
id
c0d0c8e5-4733-4714-9f89-7d7d35c0f444 (old id 1112579)
date added to LUP
2008-07-17 09:01:56
date last changed
2018-05-29 11:54:37
@article{c0d0c8e5-4733-4714-9f89-7d7d35c0f444,
  abstract     = {The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament staining after 6 days or by morphology after 4, 6 or 8 weeks. The results showed that the pseudo-nerve could induce nerve regeneration to a similar extend as a real nerve graft. The neurobiological composition of the pseudo-nerve and the factors influencing its formation were also studied. By double staining of S-100 and laminin we found that the longitudinally organized Schwann cell columns in the pseudo-nerve were surrounded by basal laminae and ensheathed by a layer of vascularized perineurium-like tissue. Macrophages (ED1 and ED2) and their products interleukin-1beta (IL-1beta) and transforming growth factor-beta1 (TGF-beta1) were constantly present in the pseudo-nerve. Besides, the size of tube was a crucial factor in influencing pseudo-nerve formation, e.g. a thicker pseudo-nerve was formed in tubes with larger diameters or shorter gap lengths. No pseudo-nerve was formed when the gap was 15 mm long. When both proximal and distal inserts were isolated nerve segments the pseudo-nerve was still formed but thin, probably because of compromised vascular supply. Taken together, the results suggested that the pseudo-nerve contains the essential neurobiological elements to induce successful axonal elongation.},
  author       = {Zhao, Q and Lundborg, Göran and Danielsen, Nils and Bjursten, Lars Magnus and Dahlin, Lars},
  issn         = {1872-6240},
  keyword      = {Nerve regeneration,Peripheral nerve,Nerve graft,Schwann cell,Silicone,Implant,Matrix,Immunohistochemistry},
  language     = {eng},
  number       = {1},
  pages        = {125--134},
  publisher    = {Elsevier},
  series       = {Brain Research1966-01-01+01:00},
  title        = {Nerve regeneration in a 'pseudo-nerve' graft created in a silicone tube},
  url          = {http://dx.doi.org/10.1016/S0006-8993(97)00620-3},
  volume       = {769},
  year         = {1997},
}