Nerve regeneration in a 'pseudo-nerve' graft created in a silicone tube
Zhao, Q ; Lundborg, Göran LU ; Danielsen, Nils LU ; Bjursten, Lars Magnus LU and Dahlin, Lars LU
(1997)
In Brain Research
769(1).
p.125-134
- Abstract
- The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament... (More)
- The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament staining after 6 days or by morphology after 4, 6 or 8 weeks. The results showed that the pseudo-nerve could induce nerve regeneration to a similar extend as a real nerve graft. The neurobiological composition of the pseudo-nerve and the factors influencing its formation were also studied. By double staining of S-100 and laminin we found that the longitudinally organized Schwann cell columns in the pseudo-nerve were surrounded by basal laminae and ensheathed by a layer of vascularized perineurium-like tissue. Macrophages (ED1 and ED2) and their products interleukin-1beta (IL-1beta) and transforming growth factor-beta1 (TGF-beta1) were constantly present in the pseudo-nerve. Besides, the size of tube was a crucial factor in influencing pseudo-nerve formation, e.g. a thicker pseudo-nerve was formed in tubes with larger diameters or shorter gap lengths. No pseudo-nerve was formed when the gap was 15 mm long. When both proximal and distal inserts were isolated nerve segments the pseudo-nerve was still formed but thin, probably because of compromised vascular supply. Taken together, the results suggested that the pseudo-nerve contains the essential neurobiological elements to induce successful axonal elongation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112579
- author
- Zhao, Q
; Lundborg, Göran
LU
; Danielsen, Nils
LU
; Bjursten, Lars Magnus
LU
and Dahlin, Lars
LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nerve regeneration, Peripheral nerve, Nerve graft, Schwann cell, Silicone, Implant, Matrix, Immunohistochemistry
- in
- Brain Research
- volume
- 769
- issue
- 1
- pages
- 125 - 134
- publisher
- Elsevier
- external identifiers
-
- pmid:9374280
- scopus:0030691095
- ISSN
- 1872-6240
- DOI
- 10.1016/S0006-8993(97)00620-3
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hand Surgery Research Group (013241910), Bioimplant Research (013242910), Neural Interfaces (013212003)
- id
- c0d0c8e5-4733-4714-9f89-7d7d35c0f444 (old id 1112579)
- date added to LUP
- 2016-04-01 12:38:06
- date last changed
- 2022-01-27 07:44:58
@article{c0d0c8e5-4733-4714-9f89-7d7d35c0f444,
abstract = {{The pseudo-nerve, which contains longitudinal Schwann cell columns without axons and surrounded by perineurium-like tissue but no axons (Q. Zhao, L.B. Dahlin, M. Kanje, G. Lundborg, Brain Res. 592 (1992) 106-114), was applied as a graft to repair nerve defect in rats. Creation of the pseudo-nerve was accomplished by inserting the proximal and distal stumps of a cut sciatic nerve into a silicone tube. The proximal insert was cut far proximally to prevent axons from entering the tube. After 4 weeks, the pseudo-nerve was harvested, trimmed into a 10-mm long graft and transplanted into a corresponding defect of the contralateral sciatic nerve. Nerve regeneration through the pseudo-nerve was examined by pinch reflex test and neurofilament staining after 6 days or by morphology after 4, 6 or 8 weeks. The results showed that the pseudo-nerve could induce nerve regeneration to a similar extend as a real nerve graft. The neurobiological composition of the pseudo-nerve and the factors influencing its formation were also studied. By double staining of S-100 and laminin we found that the longitudinally organized Schwann cell columns in the pseudo-nerve were surrounded by basal laminae and ensheathed by a layer of vascularized perineurium-like tissue. Macrophages (ED1 and ED2) and their products interleukin-1beta (IL-1beta) and transforming growth factor-beta1 (TGF-beta1) were constantly present in the pseudo-nerve. Besides, the size of tube was a crucial factor in influencing pseudo-nerve formation, e.g. a thicker pseudo-nerve was formed in tubes with larger diameters or shorter gap lengths. No pseudo-nerve was formed when the gap was 15 mm long. When both proximal and distal inserts were isolated nerve segments the pseudo-nerve was still formed but thin, probably because of compromised vascular supply. Taken together, the results suggested that the pseudo-nerve contains the essential neurobiological elements to induce successful axonal elongation.}},
author = {{Zhao, Q and Lundborg, Göran and Danielsen, Nils and Bjursten, Lars Magnus and Dahlin, Lars}},
issn = {{1872-6240}},
keywords = {{Nerve regeneration; Peripheral nerve; Nerve graft; Schwann cell; Silicone; Implant; Matrix; Immunohistochemistry}},
language = {{eng}},
number = {{1}},
pages = {{125--134}},
publisher = {{Elsevier}},
series = {{Brain Research}},
title = {{Nerve regeneration in a 'pseudo-nerve' graft created in a silicone tube}},
url = {{http://dx.doi.org/10.1016/S0006-8993(97)00620-3}},
doi = {{10.1016/S0006-8993(97)00620-3}},
volume = {{769}},
year = {{1997}},
}