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Enterostatin up-regulates the expression of the beta-subunit of F(1)F(o)-ATPase in the plasma membrane of INS-1 cells.

Lindqvist, Andreas LU ; Berger, Karin LU and Erlanson-Albertsson, Charlotte LU (2008) In Nutritional Neuroscience 11(2). p.55-60
Abstract
Exposure to high-fat diet easily promotes overeating while at the same time disrupting insulin secretion and islet function. Enterostatin is a peptide which is secreted from the pancreas in response to high-fat feeding and has been shown to inhibit fat intake as well as insulin secretion in experimental animal models. Until recently, there was no known receptor for enterostatin. In 2002, Berger and co-workers found enterostatin to target the beta-subunit of the F(1)-ATPase in rat brain membranes as well as in a clonal beta-cell line (INS-1). In this study, we found the beta-subunit of F(1)-ATPase to be ectopically expressed in the plasma membrane of INS-1 cells using both immunohistochemistry and Western blotting. Incubation with... (More)
Exposure to high-fat diet easily promotes overeating while at the same time disrupting insulin secretion and islet function. Enterostatin is a peptide which is secreted from the pancreas in response to high-fat feeding and has been shown to inhibit fat intake as well as insulin secretion in experimental animal models. Until recently, there was no known receptor for enterostatin. In 2002, Berger and co-workers found enterostatin to target the beta-subunit of the F(1)-ATPase in rat brain membranes as well as in a clonal beta-cell line (INS-1). In this study, we found the beta-subunit of F(1)-ATPase to be ectopically expressed in the plasma membrane of INS-1 cells using both immunohistochemistry and Western blotting. Incubation with enterostatin for 60 min resulted in a 3.5-fold increase of the protein expression of the beta-subunit of F(1)-ATPase in the plasma membrane. Furthermore, we found ATP to be able to displace the binding of enterostatin to purified bovine F(1)-ATPase. This reported targeting of enterostatin to the beta-subunit of F(1)-ATPase in insulin cells may provide a link between high-fat intake and islet function. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nutritional Neuroscience
volume
11
issue
2
pages
55 - 60
publisher
Taylor & Francis
external identifiers
  • wos:000256999300002
  • pmid:18510804
  • scopus:50449094038
ISSN
1476-8305
DOI
10.1179/147683008X301397
language
English
LU publication?
yes
id
c2c2be64-ced4-4d5a-a799-c92b0d6e374a (old id 1153704)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18510804?dopt=Abstract
date added to LUP
2008-06-02 10:44:26
date last changed
2017-01-01 07:37:15
@article{c2c2be64-ced4-4d5a-a799-c92b0d6e374a,
  abstract     = {Exposure to high-fat diet easily promotes overeating while at the same time disrupting insulin secretion and islet function. Enterostatin is a peptide which is secreted from the pancreas in response to high-fat feeding and has been shown to inhibit fat intake as well as insulin secretion in experimental animal models. Until recently, there was no known receptor for enterostatin. In 2002, Berger and co-workers found enterostatin to target the beta-subunit of the F(1)-ATPase in rat brain membranes as well as in a clonal beta-cell line (INS-1). In this study, we found the beta-subunit of F(1)-ATPase to be ectopically expressed in the plasma membrane of INS-1 cells using both immunohistochemistry and Western blotting. Incubation with enterostatin for 60 min resulted in a 3.5-fold increase of the protein expression of the beta-subunit of F(1)-ATPase in the plasma membrane. Furthermore, we found ATP to be able to displace the binding of enterostatin to purified bovine F(1)-ATPase. This reported targeting of enterostatin to the beta-subunit of F(1)-ATPase in insulin cells may provide a link between high-fat intake and islet function.},
  author       = {Lindqvist, Andreas and Berger, Karin and Erlanson-Albertsson, Charlotte},
  issn         = {1476-8305},
  language     = {eng},
  number       = {2},
  pages        = {55--60},
  publisher    = {Taylor & Francis},
  series       = {Nutritional Neuroscience},
  title        = {Enterostatin up-regulates the expression of the beta-subunit of F(1)F(o)-ATPase in the plasma membrane of INS-1 cells.},
  url          = {http://dx.doi.org/10.1179/147683008X301397},
  volume       = {11},
  year         = {2008},
}