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Blueberry husks and multi-strain probiotics affect colonic fermentation in rats.

Bränning, Camilla LU ; Håkansson, Åsa LU ; Ahrné, Siv LU ; Jeppsson, Bengt LU ; Molin, Göran LU and Nyman, Margareta LU (2009) In British Journal of Nutrition 100(1). p.859-870
Abstract
The aim was to investigate how blueberry husks and/or mixtures of probiotic strains (Lactobacillus crispatus DSM16743, L. gasseri DSM16737 and L. plantarum DSM15313 (LABmix), or Bifidobacterium infantis DSM15159 and DSM15161 (BIFmix)) affect colonic fermentation, caecal counts of lactobacilli, bifidobacteria and Enterobacteriaceae, body weight gain, and blood concentrations of carboxylic acids (CA) and ammonia in rats. Dietary fibres in blueberry husks were fermented to 61 % in colon, and the elevated faecal excretion of fibre and protein contributed to the high faecal bulking capacity (1.3). The caecal pool of CA was higher in rats fed blueberry husks than the fibre-free control (P < 0.05), and the propionic acid proportion was higher... (More)
The aim was to investigate how blueberry husks and/or mixtures of probiotic strains (Lactobacillus crispatus DSM16743, L. gasseri DSM16737 and L. plantarum DSM15313 (LABmix), or Bifidobacterium infantis DSM15159 and DSM15161 (BIFmix)) affect colonic fermentation, caecal counts of lactobacilli, bifidobacteria and Enterobacteriaceae, body weight gain, and blood concentrations of carboxylic acids (CA) and ammonia in rats. Dietary fibres in blueberry husks were fermented to 61 % in colon, and the elevated faecal excretion of fibre and protein contributed to the high faecal bulking capacity (1.3). The caecal pool of CA was higher in rats fed blueberry husks than the fibre-free control (P < 0.05), and the propionic acid proportion was higher in the distal colon than in the control group (P < 0.05). Probiotics lowered the caecal amount of CA when added to blueberry husks (P < 0.001), while the propionic acid proportion was higher with LABmix (P < 0.01) than blueberry husks only. The propionic acid and butyric acid concentrations in blood were higher in rats fed blueberry husks and probiotics than those fed blueberry husks only (P < 0.01), implying that the absorption of these acids was facilitated by the bacteria. The caecal counts of lactobacilli, bifidobacteria and Enterobacteriaceae were lower in rats fed blueberry husks than the control diet (P < 0.05). The body weight gain was partly influenced by the caecal tissue and contents weights, and BIFmix decreased the ammonia concentration in blood (P < 0.05). We conclude that colonic fermentation is differentially affected by dietary fibre and probiotics, which may be of importance when developing foods with certain health effects. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Weight gain, Carboxylic acids, Ammonia, Short-chain fatty acids, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus crispatus, Bifidobacterium infantis, Probiotics, Dietary fibre, Blueberry husks
in
British Journal of Nutrition
volume
100
issue
1
pages
859 - 870
publisher
Cambridge University Press
external identifiers
  • wos:000264858200014
  • pmid:18680631
  • scopus:67649419135
ISSN
1475-2662
DOI
10.1017/S0007114508047703
language
English
LU publication?
yes
id
4ac48046-7f4e-4c5e-abd3-4f7248eb4851 (old id 1223466)
date added to LUP
2008-10-20 10:13:31
date last changed
2017-12-10 03:46:07
@article{4ac48046-7f4e-4c5e-abd3-4f7248eb4851,
  abstract     = {The aim was to investigate how blueberry husks and/or mixtures of probiotic strains (Lactobacillus crispatus DSM16743, L. gasseri DSM16737 and L. plantarum DSM15313 (LABmix), or Bifidobacterium infantis DSM15159 and DSM15161 (BIFmix)) affect colonic fermentation, caecal counts of lactobacilli, bifidobacteria and Enterobacteriaceae, body weight gain, and blood concentrations of carboxylic acids (CA) and ammonia in rats. Dietary fibres in blueberry husks were fermented to 61 % in colon, and the elevated faecal excretion of fibre and protein contributed to the high faecal bulking capacity (1.3). The caecal pool of CA was higher in rats fed blueberry husks than the fibre-free control (P &lt; 0.05), and the propionic acid proportion was higher in the distal colon than in the control group (P &lt; 0.05). Probiotics lowered the caecal amount of CA when added to blueberry husks (P &lt; 0.001), while the propionic acid proportion was higher with LABmix (P &lt; 0.01) than blueberry husks only. The propionic acid and butyric acid concentrations in blood were higher in rats fed blueberry husks and probiotics than those fed blueberry husks only (P &lt; 0.01), implying that the absorption of these acids was facilitated by the bacteria. The caecal counts of lactobacilli, bifidobacteria and Enterobacteriaceae were lower in rats fed blueberry husks than the control diet (P &lt; 0.05). The body weight gain was partly influenced by the caecal tissue and contents weights, and BIFmix decreased the ammonia concentration in blood (P &lt; 0.05). We conclude that colonic fermentation is differentially affected by dietary fibre and probiotics, which may be of importance when developing foods with certain health effects.},
  author       = {Bränning, Camilla and Håkansson, Åsa and Ahrné, Siv and Jeppsson, Bengt and Molin, Göran and Nyman, Margareta},
  issn         = {1475-2662},
  keyword      = {Weight gain,Carboxylic acids,Ammonia,Short-chain fatty acids,Lactobacillus gasseri,Lactobacillus plantarum,Lactobacillus crispatus,Bifidobacterium infantis,Probiotics,Dietary fibre,Blueberry husks},
  language     = {eng},
  number       = {1},
  pages        = {859--870},
  publisher    = {Cambridge University Press},
  series       = {British Journal of Nutrition},
  title        = {Blueberry husks and multi-strain probiotics affect colonic fermentation in rats.},
  url          = {http://dx.doi.org/10.1017/S0007114508047703},
  volume       = {100},
  year         = {2009},
}