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Nontypeable Haemophilus influenzae Adhesin Protein E: Characterization and Biological Activity.

Ronander, Elena LU ; Brant, Marta LU ; Eriksson, Emily LU ; Mörgelin, Matthias LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2009) In Journal of Infectious Diseases 199. p.522-531
Abstract
The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on... (More)
The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
199
pages
522 - 531
publisher
Oxford University Press
external identifiers
  • wos:000262802200008
  • pmid:19125675
  • scopus:61849119539
ISSN
1537-6613
DOI
10.1086/596211
language
English
LU publication?
yes
id
1a769c5d-ad5c-40b3-b456-6a41398b817e (old id 1289926)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19125675?dopt=Abstract
date added to LUP
2009-02-03 10:29:51
date last changed
2017-03-19 04:21:28
@article{1a769c5d-ad5c-40b3-b456-6a41398b817e,
  abstract     = {The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection.},
  author       = {Ronander, Elena and Brant, Marta and Eriksson, Emily and Mörgelin, Matthias and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1537-6613},
  language     = {eng},
  pages        = {522--531},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Nontypeable Haemophilus influenzae Adhesin Protein E: Characterization and Biological Activity.},
  url          = {http://dx.doi.org/10.1086/596211},
  volume       = {199},
  year         = {2009},
}