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Isothermal calorimetry approach to evaluate tissue damage in carrot slices upon thermal processing

Gomez, Federico LU ; Toledo, RT; Wadsö, Lars LU ; Gekas, Vassilis LU and Sjöholm, Ingegerd LU (2004) In Journal of Food Engineering 65(2). p.165-173
Abstract
The purpose of this investigation was to test calorimetry as a means of quantifying cell damage occurring at various stages of mild blanching treatments. Calorimetric measurements were carried out on cores taken from carrot slices blanched at 100 degreesC for 5-45 s. The calorimetric data were corrected for the influence of wounding stress due to slicing and coring. The validity of calorimetry to measure cell damage was assessed by simulating inactivation of cell metabolism based on bibliographic data on thermal inactivation kinetics of plasma membrane H+-ATPase, used as a marker of overall cell membrane damage. Calorimetric data paralleled the simulated decrease of actively metabolizing cells only in the first 20 s of blanching but... (More)
The purpose of this investigation was to test calorimetry as a means of quantifying cell damage occurring at various stages of mild blanching treatments. Calorimetric measurements were carried out on cores taken from carrot slices blanched at 100 degreesC for 5-45 s. The calorimetric data were corrected for the influence of wounding stress due to slicing and coring. The validity of calorimetry to measure cell damage was assessed by simulating inactivation of cell metabolism based on bibliographic data on thermal inactivation kinetics of plasma membrane H+-ATPase, used as a marker of overall cell membrane damage. Calorimetric data paralleled the simulated decrease of actively metabolizing cells only in the first 20 s of blanching but diverged at higher blanching times. Our results suggest that inactivation of enzymes responsible for quality-related changes during frozen storage (e.g. blanching at 100 degreesC for 20 s) may be accompanied by cell damage in about 70% of the tissue. (C) 2004 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Food Engineering
volume
65
issue
2
pages
165 - 173
publisher
Elsevier
external identifiers
  • wos:000222489700002
  • scopus:2642562950
ISSN
0260-8774
DOI
10.1016/j.jfoodeng.2004.01.010
language
English
LU publication?
yes
id
14e0d365-c6aa-4cea-bec1-9bf6742bc31e (old id 139186)
date added to LUP
2007-07-05 09:01:52
date last changed
2017-03-12 04:13:15
@article{14e0d365-c6aa-4cea-bec1-9bf6742bc31e,
  abstract     = {The purpose of this investigation was to test calorimetry as a means of quantifying cell damage occurring at various stages of mild blanching treatments. Calorimetric measurements were carried out on cores taken from carrot slices blanched at 100 degreesC for 5-45 s. The calorimetric data were corrected for the influence of wounding stress due to slicing and coring. The validity of calorimetry to measure cell damage was assessed by simulating inactivation of cell metabolism based on bibliographic data on thermal inactivation kinetics of plasma membrane H+-ATPase, used as a marker of overall cell membrane damage. Calorimetric data paralleled the simulated decrease of actively metabolizing cells only in the first 20 s of blanching but diverged at higher blanching times. Our results suggest that inactivation of enzymes responsible for quality-related changes during frozen storage (e.g. blanching at 100 degreesC for 20 s) may be accompanied by cell damage in about 70% of the tissue. (C) 2004 Elsevier Ltd. All rights reserved.},
  author       = {Gomez, Federico and Toledo, RT and Wadsö, Lars and Gekas, Vassilis and Sjöholm, Ingegerd},
  issn         = {0260-8774},
  language     = {eng},
  number       = {2},
  pages        = {165--173},
  publisher    = {Elsevier},
  series       = {Journal of Food Engineering},
  title        = {Isothermal calorimetry approach to evaluate tissue damage in carrot slices upon thermal processing},
  url          = {http://dx.doi.org/10.1016/j.jfoodeng.2004.01.010},
  volume       = {65},
  year         = {2004},
}