Immunohistochemical detection of tyrosine phosphatase SHP-1 predicts outcome after radical prostatectomy for localized prostate cancer.
(2010) In International Journal of Cancer 126. p.2296-2307- Abstract
- The protein tyrosine kinase (PTK) receptors and cytosolic signaling proteins as well as the protein tyrosine phosphatases (PTPs) have important roles in regulation of growth of the benign and malignant prostate gland. Here we studied expression of the protein tyrosine phosphatase SHP-1 in prostate cancer cell lines and in human prostatic tissues. SHP-1 is expressed at a high level in LNCaP prostate cancer cells compared with PC3 cells. Silencing of SHP-1 expression with siRNA in LNCaP cells led to an increased rate of proliferation, whereas overexpression of SHP-1 by means of transient transfection in PC3 cells led to a decrease in proliferation. Corresponding changes were observed in cyclin D1 expression. We further demonstrate that LNCaP... (More)
- The protein tyrosine kinase (PTK) receptors and cytosolic signaling proteins as well as the protein tyrosine phosphatases (PTPs) have important roles in regulation of growth of the benign and malignant prostate gland. Here we studied expression of the protein tyrosine phosphatase SHP-1 in prostate cancer cell lines and in human prostatic tissues. SHP-1 is expressed at a high level in LNCaP prostate cancer cells compared with PC3 cells. Silencing of SHP-1 expression with siRNA in LNCaP cells led to an increased rate of proliferation, whereas overexpression of SHP-1 by means of transient transfection in PC3 cells led to a decrease in proliferation. Corresponding changes were observed in cyclin D1 expression. We further demonstrate that LNCaP and PC3 cells respond differently to IL-6 stimulation. SHP-1 overexpression in PC3 cells reversed IL-6 stimulation of proliferation whereas in SHP-1-silenced LNCaP cells IL-6 inhibition of proliferation was not affected. In addition, IL-6 treatment led to higher levels of phosphorylated STAT3 in SHP-1-silenced LNCaP cells than in control cells. Next, SHP-1 expression in human prostate cancer was analyzed by immunohistochemical staining of tissue microarrays comprising tumor specimens from 100 prostate cancer patients. We found an inverse correlation between the tumor level of SHP-1 expression and time to biochemical recurrence and clinical progression among prostate cancer patients. In conclusion, our results suggest that a decreased level of SHP-1 expression in prostate cancer cells is associated with a high proliferation rate and an increased risk of recurrence or clinical progression after radical prostatectomy for localized prostate cancer. (c) 2009 UICC. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1500822
- author
- Tassidis, Helena
LU
; Brokken, Leon
LU
; Jirström, Karin
LU
; Ehrnström, Roy LU ; Pontén, Fredrik LU ; Ulmert, David LU ; Bjartell, Anders LU ; Härkönen, Pirkko LU and Gjörloff Wingren, Anette LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Cancer
- volume
- 126
- pages
- 2296 - 2307
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000276928700004
- pmid:19795453
- scopus:77951239460
- pmid:19795453
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.24917
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Urological Cancers (013243420), Clinical Chemistry, Malmö (013016000), Pure and Applied Biochemistry (LTH) (011001005), Pathology (Malmö) (013031000), Tumour Biology, Malmö (013031300), Pathology, (Lund) (013030000) Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:26.
- id
- 14e3a067-d3a7-4473-a6b4-44fa30e5e54d (old id 1500822)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19795453?dopt=Abstract
- date added to LUP
- 2016-04-04 08:39:21
- date last changed
- 2025-01-20 04:42:53
@article{14e3a067-d3a7-4473-a6b4-44fa30e5e54d, abstract = {{The protein tyrosine kinase (PTK) receptors and cytosolic signaling proteins as well as the protein tyrosine phosphatases (PTPs) have important roles in regulation of growth of the benign and malignant prostate gland. Here we studied expression of the protein tyrosine phosphatase SHP-1 in prostate cancer cell lines and in human prostatic tissues. SHP-1 is expressed at a high level in LNCaP prostate cancer cells compared with PC3 cells. Silencing of SHP-1 expression with siRNA in LNCaP cells led to an increased rate of proliferation, whereas overexpression of SHP-1 by means of transient transfection in PC3 cells led to a decrease in proliferation. Corresponding changes were observed in cyclin D1 expression. We further demonstrate that LNCaP and PC3 cells respond differently to IL-6 stimulation. SHP-1 overexpression in PC3 cells reversed IL-6 stimulation of proliferation whereas in SHP-1-silenced LNCaP cells IL-6 inhibition of proliferation was not affected. In addition, IL-6 treatment led to higher levels of phosphorylated STAT3 in SHP-1-silenced LNCaP cells than in control cells. Next, SHP-1 expression in human prostate cancer was analyzed by immunohistochemical staining of tissue microarrays comprising tumor specimens from 100 prostate cancer patients. We found an inverse correlation between the tumor level of SHP-1 expression and time to biochemical recurrence and clinical progression among prostate cancer patients. In conclusion, our results suggest that a decreased level of SHP-1 expression in prostate cancer cells is associated with a high proliferation rate and an increased risk of recurrence or clinical progression after radical prostatectomy for localized prostate cancer. (c) 2009 UICC.}}, author = {{Tassidis, Helena and Brokken, Leon and Jirström, Karin and Ehrnström, Roy and Pontén, Fredrik and Ulmert, David and Bjartell, Anders and Härkönen, Pirkko and Gjörloff Wingren, Anette}}, issn = {{0020-7136}}, language = {{eng}}, pages = {{2296--2307}}, publisher = {{John Wiley & Sons Inc.}}, series = {{International Journal of Cancer}}, title = {{Immunohistochemical detection of tyrosine phosphatase SHP-1 predicts outcome after radical prostatectomy for localized prostate cancer.}}, url = {{http://dx.doi.org/10.1002/ijc.24917}}, doi = {{10.1002/ijc.24917}}, volume = {{126}}, year = {{2010}}, }