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Hollow fibre-supported liquid membrane extraction and LC-MS/MS detection for the analysis of heterocyclic amines in urine samples

Busquets, Rosa ; Jönsson, Jan Åke LU ; Frandsen, Henrik ; Puignou, Lluis ; Teresa Galceran, Maria and Skog, Kerstin LU (2009) In Molecular Nutrition and Food Research 53(12). p.1496-1504
Abstract
Heterocyclic amines (HCAs) are potent mutagens/carcinogens to which humans are frequently exposed through the consumption of cooked meat and fish food. The effect of normal intake of HCAs and their role in the aetiology of human cancer is unknown. To some extent, limitations of the existing analytical methods in monitoring the low levels of HCAs in biological samples have hindered obtaining conclusive results. In this study, a method for the analysis of HCAs in human urine has been studied to detect HCAs and metabolites at levels resulting from consumption of food cooked at ordinary conditions. The analytical method consisted of extraction and clean-Lip by the novel technique liquid-phase microextraction combined with LC-MS/MS. The effect... (More)
Heterocyclic amines (HCAs) are potent mutagens/carcinogens to which humans are frequently exposed through the consumption of cooked meat and fish food. The effect of normal intake of HCAs and their role in the aetiology of human cancer is unknown. To some extent, limitations of the existing analytical methods in monitoring the low levels of HCAs in biological samples have hindered obtaining conclusive results. In this study, a method for the analysis of HCAs in human urine has been studied to detect HCAs and metabolites at levels resulting from consumption of food cooked at ordinary conditions. The analytical method consisted of extraction and clean-Lip by the novel technique liquid-phase microextraction combined with LC-MS/MS. The effect of pH during the extraction and hydrolysis step was examined. High sensitivity was achieved when the extraction was performed in raw urine adjusted to pH 5.5, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine being detected from 2 pg/g urine, levels comparable with a normal exposure. Good reproducibility and repeat-ability was obtained for 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, below 9% using isotopic dilution. The performance of the method on 9H-pyrido[3,4-b]indole, 2-amino-1-methyl-6-(4'-hydroxyphenyl)imidazo[4,5-b]pyridine and 2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine was also studied. (Less)
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published
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keywords
Heterocyclic amines, 5-b]pyridine, 2-Amino-1-methyl-6-(4 '-hydroxyphenyl)imidazo[4, 2-Amino-1-methyl-6-(5-hydroxy)phenylimidazo[4, Liquid-phase microextraction, Urine
in
Molecular Nutrition and Food Research
volume
53
issue
12
pages
1496 - 1504
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000273183800003
  • scopus:71149107268
ISSN
1613-4133
DOI
10.1002/mnfr.200800577
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Applied Nutrition and Food Chemistry (011001300)
id
2f195fbb-bb3e-4319-94d6-0fa02aafc4b9 (old id 1531567)
date added to LUP
2016-04-01 12:07:49
date last changed
2021-08-04 01:33:05
@article{2f195fbb-bb3e-4319-94d6-0fa02aafc4b9,
  abstract     = {Heterocyclic amines (HCAs) are potent mutagens/carcinogens to which humans are frequently exposed through the consumption of cooked meat and fish food. The effect of normal intake of HCAs and their role in the aetiology of human cancer is unknown. To some extent, limitations of the existing analytical methods in monitoring the low levels of HCAs in biological samples have hindered obtaining conclusive results. In this study, a method for the analysis of HCAs in human urine has been studied to detect HCAs and metabolites at levels resulting from consumption of food cooked at ordinary conditions. The analytical method consisted of extraction and clean-Lip by the novel technique liquid-phase microextraction combined with LC-MS/MS. The effect of pH during the extraction and hydrolysis step was examined. High sensitivity was achieved when the extraction was performed in raw urine adjusted to pH 5.5, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine being detected from 2 pg/g urine, levels comparable with a normal exposure. Good reproducibility and repeat-ability was obtained for 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, below 9% using isotopic dilution. The performance of the method on 9H-pyrido[3,4-b]indole, 2-amino-1-methyl-6-(4'-hydroxyphenyl)imidazo[4,5-b]pyridine and 2-amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine was also studied.},
  author       = {Busquets, Rosa and Jönsson, Jan Åke and Frandsen, Henrik and Puignou, Lluis and Teresa Galceran, Maria and Skog, Kerstin},
  issn         = {1613-4133},
  language     = {eng},
  number       = {12},
  pages        = {1496--1504},
  publisher    = {John Wiley & Sons Inc.},
  series       = {Molecular Nutrition and Food Research},
  title        = {Hollow fibre-supported liquid membrane extraction and LC-MS/MS detection for the analysis of heterocyclic amines in urine samples},
  url          = {http://dx.doi.org/10.1002/mnfr.200800577},
  doi          = {10.1002/mnfr.200800577},
  volume       = {53},
  year         = {2009},
}