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Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease.

Li, Jia-Yi LU ; Englund, Elisabet LU ; Widner, Håkan LU ; Rehncrona, Stig LU ; Björklund, Anders LU ; Lindvall, Olle LU and Brundin, Patrik LU (2010) In Movement Disorders 25(8). p.1091-1096
Abstract
We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in... (More)
We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra. (c) 2010 Movement Disorder Society. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neural transplantation, transmissible neurological disease, Lewy body, alpha-synuclein, protein aggregation
in
Movement Disorders
volume
25
issue
8
pages
1091 - 1096
publisher
John Wiley & Sons
external identifiers
  • wos:000279044600019
  • pmid:20198645
  • scopus:77949820437
ISSN
0885-3185
DOI
10.1002/mds.23012
language
English
LU publication?
yes
id
40867eef-c7f9-449b-8e0e-5a0a56c75550 (old id 1582610)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20198645?dopt=Abstract
date added to LUP
2010-04-07 11:23:13
date last changed
2018-06-17 03:25:43
@article{40867eef-c7f9-449b-8e0e-5a0a56c75550,
  abstract     = {We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra. (c) 2010 Movement Disorder Society.},
  author       = {Li, Jia-Yi and Englund, Elisabet and Widner, Håkan and Rehncrona, Stig and Björklund, Anders and Lindvall, Olle and Brundin, Patrik},
  issn         = {0885-3185},
  keyword      = {neural transplantation,transmissible neurological disease,Lewy body,alpha-synuclein,protein aggregation},
  language     = {eng},
  number       = {8},
  pages        = {1091--1096},
  publisher    = {John Wiley & Sons},
  series       = {Movement Disorders},
  title        = {Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease.},
  url          = {http://dx.doi.org/10.1002/mds.23012},
  volume       = {25},
  year         = {2010},
}