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Bioactive Suture with Added Innate Defense Functionality for the Reduction of Bacterial Infection and Inflammation

Puthia, Manoj LU ; Petrlova, Jitka LU ; Petruk, Ganna LU orcid ; Butrym, Marta LU ; Samsudin, Firdaus ; Andersson, Madeleine Å LU ; Strömdahl, Ann-Charlotte LU ; Wasserstrom, Sebastian LU ; Hartman, Erik and Kjellström, Sven LU , et al. (2023) In Advanced healthcare materials 12(31).
Abstract
Surgical site infections (SSI) are a clinical and economic burden. Suture-associated SSI may develop when bacteria colonize the suture surface and form biofilms that are resistant to antibiotics. Thrombin-derived C-terminal peptide (TCP)-25 is a host defense peptide with a unique dual mode of action that can target both bacteria and the excessive inflammation induced by bacterial products. The peptide demonstrates therapeutic potential in preclinical in vivo wound infection models. In this study, the authors set out to explore whether TCP-25 can provide a new bioactive innate immune feature to hydrophilic polyglactin sutures (Vicryl). Using a combination of biochemical, biophysical, antibacterial, biofilm, and anti-inflammatory assays in... (More)
Surgical site infections (SSI) are a clinical and economic burden. Suture-associated SSI may develop when bacteria colonize the suture surface and form biofilms that are resistant to antibiotics. Thrombin-derived C-terminal peptide (TCP)-25 is a host defense peptide with a unique dual mode of action that can target both bacteria and the excessive inflammation induced by bacterial products. The peptide demonstrates therapeutic potential in preclinical in vivo wound infection models. In this study, the authors set out to explore whether TCP-25 can provide a new bioactive innate immune feature to hydrophilic polyglactin sutures (Vicryl). Using a combination of biochemical, biophysical, antibacterial, biofilm, and anti-inflammatory assays in vitro, in silico molecular modeling studies, along with experimental infection and inflammation models in mice, a proof-of-concept that TCP-25 can provide Vicryl sutures with a previously undisclosed host defense capacity, that enables targeting of bacteria, biofilms, and the accompanying inflammatory response, is shown. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Advanced healthcare materials
volume
12
issue
31
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85171534958
  • pmid:37689972
ISSN
2192-2659
DOI
10.1002/adhm.202300987
language
English
LU publication?
yes
id
16901188-cefb-4e6b-91dd-2065545fd899
date added to LUP
2023-11-12 22:18:51
date last changed
2024-01-09 15:45:08
@article{16901188-cefb-4e6b-91dd-2065545fd899,
  abstract     = {{Surgical site infections (SSI) are a clinical and economic burden. Suture-associated SSI may develop when bacteria colonize the suture surface and form biofilms that are resistant to antibiotics. Thrombin-derived C-terminal peptide (TCP)-25 is a host defense peptide with a unique dual mode of action that can target both bacteria and the excessive inflammation induced by bacterial products. The peptide demonstrates therapeutic potential in preclinical in vivo wound infection models. In this study, the authors set out to explore whether TCP-25 can provide a new bioactive innate immune feature to hydrophilic polyglactin sutures (Vicryl). Using a combination of biochemical, biophysical, antibacterial, biofilm, and anti-inflammatory assays in vitro, in silico molecular modeling studies, along with experimental infection and inflammation models in mice, a proof-of-concept that TCP-25 can provide Vicryl sutures with a previously undisclosed host defense capacity, that enables targeting of bacteria, biofilms, and the accompanying inflammatory response, is shown.}},
  author       = {{Puthia, Manoj and Petrlova, Jitka and Petruk, Ganna and Butrym, Marta and Samsudin, Firdaus and Andersson, Madeleine Å and Strömdahl, Ann-Charlotte and Wasserstrom, Sebastian and Hartman, Erik and Kjellström, Sven and Caselli, Lucrezia and Klementieva, Oxana and Bond, Peter J. and Malmsten, Martin and Raina, Deepak and Schmidtchen, Artur}},
  issn         = {{2192-2659}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{31}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Advanced healthcare materials}},
  title        = {{Bioactive Suture with Added Innate Defense Functionality for the Reduction of Bacterial Infection and Inflammation}},
  url          = {{http://dx.doi.org/10.1002/adhm.202300987}},
  doi          = {{10.1002/adhm.202300987}},
  volume       = {{12}},
  year         = {{2023}},
}