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Feeding appetite suppressing thylakoids to pigs alters pancreatic lipase/colipase secretion

Köhnke, Rickard LU ; Svensson, Linnea; Piedra, Jose L. Valverde; Pierzynowski, Stefan LU ; Weström, Björn LU and Erlanson-Albertsson, Charlotte LU (2010) 11th International Symposium on Digestive Physiology of Pigs In Livestock Science 134(1-3). p.68-71
Abstract
The mechanism for a new appetite suppressor named thylakoids (membrane proteins derived from spinach leaves) was examined in vivo in pigs. Thylakoids inhibit the lipase/colipase hydrolysis of triacylglycerols (TG) in vitro and suppress food intake, decrease body weight gain and raise the circulating satiety hormone cholecystokinin (CCK) in rats but its mechanism in vivo remains unclear. We hypothesized that a thylakoid-enriched diet prolongs intestinal digestion of food and therefore promote satiety signaling. Five pigs were surgically prepared with a fistula in the duodenum for collection of digesta and with two catheters, one in v. jugularis and one in v. porta, for blood collection. After 1 week of recovery and an overnight fast the... (More)
The mechanism for a new appetite suppressor named thylakoids (membrane proteins derived from spinach leaves) was examined in vivo in pigs. Thylakoids inhibit the lipase/colipase hydrolysis of triacylglycerols (TG) in vitro and suppress food intake, decrease body weight gain and raise the circulating satiety hormone cholecystokinin (CCK) in rats but its mechanism in vivo remains unclear. We hypothesized that a thylakoid-enriched diet prolongs intestinal digestion of food and therefore promote satiety signaling. Five pigs were surgically prepared with a fistula in the duodenum for collection of digesta and with two catheters, one in v. jugularis and one in v. porta, for blood collection. After 1 week of recovery and an overnight fast the pigs were fed a high-fat diet with and without supplementation with thylakoids. Duodenal content and blood samples were taken before and 15, 30, 60, 120, 240 and 360 min after feeding. Pancreatic lipase and colipase enzymes were measured in duodenal digesta. Blood samples were analyzed for the satiety hormone CCK as well as insulin and glucose. We found that pancreatic lipase/colipase level increased and stayed elevated for a longer time in the duodenum in the pigs receiving thylakoids compared to the control. CCK levels were unchanged. Insulin levels were significantly reduced by the thylakoid treatment without any change in blood glucose. In conclusion, thylakoids increased lipase/colipase secretion. The mechanism for this secretion appears not to be related to CCK and may be an effect of vagal activation. Thylakoids gave reduced insulin levels without any change in glucose levels. (C) 2010 Published by Elsevier B.V. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Satiety, Glucose, Food intake, CCK, Insulin
in
Livestock Science
volume
134
issue
1-3
pages
68 - 71
publisher
Elsevier
conference name
11th International Symposium on Digestive Physiology of Pigs
external identifiers
  • wos:000284025800021
  • scopus:77957118471
ISSN
1871-1413
DOI
10.1016/j.livsci.2010.06.100
language
English
LU publication?
yes
id
bfb33c41-b5ec-4bde-bf9c-c33f6177b0be (old id 1753119)
date added to LUP
2010-12-29 14:47:06
date last changed
2018-05-29 11:47:59
@inproceedings{bfb33c41-b5ec-4bde-bf9c-c33f6177b0be,
  abstract     = {The mechanism for a new appetite suppressor named thylakoids (membrane proteins derived from spinach leaves) was examined in vivo in pigs. Thylakoids inhibit the lipase/colipase hydrolysis of triacylglycerols (TG) in vitro and suppress food intake, decrease body weight gain and raise the circulating satiety hormone cholecystokinin (CCK) in rats but its mechanism in vivo remains unclear. We hypothesized that a thylakoid-enriched diet prolongs intestinal digestion of food and therefore promote satiety signaling. Five pigs were surgically prepared with a fistula in the duodenum for collection of digesta and with two catheters, one in v. jugularis and one in v. porta, for blood collection. After 1 week of recovery and an overnight fast the pigs were fed a high-fat diet with and without supplementation with thylakoids. Duodenal content and blood samples were taken before and 15, 30, 60, 120, 240 and 360 min after feeding. Pancreatic lipase and colipase enzymes were measured in duodenal digesta. Blood samples were analyzed for the satiety hormone CCK as well as insulin and glucose. We found that pancreatic lipase/colipase level increased and stayed elevated for a longer time in the duodenum in the pigs receiving thylakoids compared to the control. CCK levels were unchanged. Insulin levels were significantly reduced by the thylakoid treatment without any change in blood glucose. In conclusion, thylakoids increased lipase/colipase secretion. The mechanism for this secretion appears not to be related to CCK and may be an effect of vagal activation. Thylakoids gave reduced insulin levels without any change in glucose levels. (C) 2010 Published by Elsevier B.V.},
  author       = {Köhnke, Rickard and Svensson, Linnea and Piedra, Jose L. Valverde and Pierzynowski, Stefan and Weström, Björn and Erlanson-Albertsson, Charlotte},
  booktitle    = {Livestock Science},
  issn         = {1871-1413},
  keyword      = {Satiety,Glucose,Food intake,CCK,Insulin},
  language     = {eng},
  number       = {1-3},
  pages        = {68--71},
  publisher    = {Elsevier},
  title        = {Feeding appetite suppressing thylakoids to pigs alters pancreatic lipase/colipase secretion},
  url          = {http://dx.doi.org/10.1016/j.livsci.2010.06.100},
  volume       = {134},
  year         = {2010},
}