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Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis

Cifani, Paolo LU ; Bendz, Maria; Wårell, Kristofer LU ; Hansson, Karin M LU ; Levander, Fredrik LU ; Sandin, Marianne LU ; Krogh, Morten LU ; Ovenberger, Marie LU ; Fredlund, Erik LU and Vaapil, Marica LU , et al. (2011) In Journal of Proteome Research 10(4). p.1645-1656
Abstract
Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.
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Contribution to journal
publication status
published
subject
keywords
isotopic labeling, HPLC-MS, 2D-PAGE, plasma membrane, protein, expression, mRNA expression, correlation, hypoxia, normoxia, neuroblastoma
in
Journal of Proteome Research
volume
10
issue
4
pages
1645 - 1656
publisher
The American Chemical Society
external identifiers
  • wos:000288924000019
  • scopus:79953720622
ISSN
1535-3893
DOI
10.1021/pr100982j
language
English
LU publication?
yes
id
49a3c3fd-3070-447a-8901-c290b1efbe76 (old id 1925248)
date added to LUP
2011-05-02 10:01:08
date last changed
2017-06-25 03:21:51
@article{49a3c3fd-3070-447a-8901-c290b1efbe76,
  abstract     = {Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.},
  author       = {Cifani, Paolo and Bendz, Maria and Wårell, Kristofer and Hansson, Karin M and Levander, Fredrik and Sandin, Marianne and Krogh, Morten and Ovenberger, Marie and Fredlund, Erik and Vaapil, Marica and Pietras, Alexander and Påhlman, Sven and James, Peter},
  issn         = {1535-3893},
  keyword      = {isotopic labeling,HPLC-MS,2D-PAGE,plasma membrane,protein,expression,mRNA expression,correlation,hypoxia,normoxia,neuroblastoma},
  language     = {eng},
  number       = {4},
  pages        = {1645--1656},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis},
  url          = {http://dx.doi.org/10.1021/pr100982j},
  volume       = {10},
  year         = {2011},
}