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TGF-beta enhances the production of hyaluronan in human lung but not in skin fibroblasts

Westergren-Thorsson, G LU ; Särnstrand, B LU ; Fransson, L A LU and Malmström, A LU (1990) In Experimental Cell Research 186(1). p.5-192
Abstract

Transforming growth factor-beta (TGF-beta) enhances the production of extracellular matrix components, such as type I and type III collagen, fibronectin, proteoglycans, in various cell types. The effect on hyaluronan synthesis in relation to proteoglycan synthesis has not been investigated. Human lung or skin fibroblast cultures were treated with TGF-beta in serum-free medium for various periods of time. 35SO4 or [3H]glucosamine was then added to the cultures in the absence of TGF-beta for up to 48 h. Hyaluronan and proteoglycans were isolated by ion-exchange chromatography and quantitated. TGF-beta induced a three- to fourfold increase in hyaluronan production by lung cells but had no effect on skin fibroblasts. In contrast,... (More)

Transforming growth factor-beta (TGF-beta) enhances the production of extracellular matrix components, such as type I and type III collagen, fibronectin, proteoglycans, in various cell types. The effect on hyaluronan synthesis in relation to proteoglycan synthesis has not been investigated. Human lung or skin fibroblast cultures were treated with TGF-beta in serum-free medium for various periods of time. 35SO4 or [3H]glucosamine was then added to the cultures in the absence of TGF-beta for up to 48 h. Hyaluronan and proteoglycans were isolated by ion-exchange chromatography and quantitated. TGF-beta induced a three- to fourfold increase in hyaluronan production by lung cells but had no effect on skin fibroblasts. In contrast, proteoglycan synthesis was enhanced in both cell types, although skin fibroblasts responded at lower concentrations of TGF-beta. Increased accumulation of hyaluronan was noted only in the cell medium, whereas proteoglycan accumulation was observed both in the medium and in the cell layer. The ED50 for TGF-beta on hyaluronan accumulation in lung cells was the same as that for proteoglycan accumulation, i.e., 40 pM. In skin fibroblasts the ED50 was considerably lower (4 pM). The induction time needed to attain full effect of TGF-beta was 6 h for both hyaluronan and proteoglycan synthesis. These results indicate that TGF-beta has tissue-specific effects on matrix production which may be of importance for control of cell proliferation in various disease states.

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organization
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Contribution to journal
publication status
published
subject
keywords
Cells, Cultured, Fibroblasts, Humans, Hyaluronic Acid, Lung, Proteoglycans, Skin, Transforming Growth Factors, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Cell Research
volume
186
issue
1
pages
5 - 192
publisher
Academic Press
external identifiers
  • scopus:0025159210
ISSN
0014-4827
DOI
10.1016/0014-4827(90)90227-2
language
English
LU publication?
yes
id
1bcafa7d-7607-4d09-87af-923e018d84ff
date added to LUP
2017-06-27 14:00:54
date last changed
2017-07-02 05:03:51
@article{1bcafa7d-7607-4d09-87af-923e018d84ff,
  abstract     = {<p>Transforming growth factor-beta (TGF-beta) enhances the production of extracellular matrix components, such as type I and type III collagen, fibronectin, proteoglycans, in various cell types. The effect on hyaluronan synthesis in relation to proteoglycan synthesis has not been investigated. Human lung or skin fibroblast cultures were treated with TGF-beta in serum-free medium for various periods of time. 35SO4 or [3H]glucosamine was then added to the cultures in the absence of TGF-beta for up to 48 h. Hyaluronan and proteoglycans were isolated by ion-exchange chromatography and quantitated. TGF-beta induced a three- to fourfold increase in hyaluronan production by lung cells but had no effect on skin fibroblasts. In contrast, proteoglycan synthesis was enhanced in both cell types, although skin fibroblasts responded at lower concentrations of TGF-beta. Increased accumulation of hyaluronan was noted only in the cell medium, whereas proteoglycan accumulation was observed both in the medium and in the cell layer. The ED50 for TGF-beta on hyaluronan accumulation in lung cells was the same as that for proteoglycan accumulation, i.e., 40 pM. In skin fibroblasts the ED50 was considerably lower (4 pM). The induction time needed to attain full effect of TGF-beta was 6 h for both hyaluronan and proteoglycan synthesis. These results indicate that TGF-beta has tissue-specific effects on matrix production which may be of importance for control of cell proliferation in various disease states.</p>},
  author       = {Westergren-Thorsson, G and Särnstrand, B and Fransson, L A and Malmström, A},
  issn         = {0014-4827},
  keyword      = {Cells, Cultured,Fibroblasts,Humans,Hyaluronic Acid,Lung,Proteoglycans,Skin,Transforming Growth Factors,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {5--192},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {TGF-beta enhances the production of hyaluronan in human lung but not in skin fibroblasts},
  url          = {http://dx.doi.org/10.1016/0014-4827(90)90227-2},
  volume       = {186},
  year         = {1990},
}