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Lingonberries alter the gut microbiota and prevent low-grade inflammation in high-fat diet fed mice

Heyman Lindén, Lovisa; Kotowska, Dorota LU ; Sand, Elin; Mikael, Bjursell; Plaza, Merichel LU ; Turner, Charlotta LU ; Holm, Cecilia LU ; Fåk, Frida LU and Berger, Karin LU (2016) In Food & Nutrition Research 60.
Abstract

Background: The gut microbiota plays an important role in the development of obesity and obesity-associated impairments such as low-grade inflammation. Lingonberries have been shown to prevent diet-induced obesity and low-grade inflammation. However, it is not known whether the effect of lingonberry supplementation is related to modifications of the gut microbiota. The aim of the present study was to describe whether consumption of different batches of lingonberries alters the composition of the gut microbiota, which could be relevant for the protective effect against high fat (HF)-induced metabolic alterations. Methods: Three groups of C57BL/6J mice were fed HF diet with or without a supplement of 20% lingonberries from two different... (More)

Background: The gut microbiota plays an important role in the development of obesity and obesity-associated impairments such as low-grade inflammation. Lingonberries have been shown to prevent diet-induced obesity and low-grade inflammation. However, it is not known whether the effect of lingonberry supplementation is related to modifications of the gut microbiota. The aim of the present study was to describe whether consumption of different batches of lingonberries alters the composition of the gut microbiota, which could be relevant for the protective effect against high fat (HF)-induced metabolic alterations. Methods: Three groups of C57BL/6J mice were fed HF diet with or without a supplement of 20% lingonberries from two different batches (Lingon1 and Lingon2) during 11 weeks. The composition and functionality of the cecal microbiota were assessed by 16S rRNA sequencing and PICRUSt. In addition, parameters related to obesity, insulin sensitivity, hepatic steatosis, inflammation and gut barrier function were examined. Results: HF-induced obesity was only prevented by the Lingon1 diet, whereas both batches of lingonberries reduced plasma levels of markers of inflammation and endotoxemia (SAA and LBP) as well as modified the composition and functionality of the gut microbiota, compared to the HF control group. The relative abundance of Akkermansia and Faecalibacterium, genera associated with healthy gut mucosa and antiinflammation, was found to increase in response to lingonberry intake. Conclusions: Our results show that supplementation with lingonberries to an HF diet prevents low-grade inflammation and is associated with significant changes of the microbiota composition. Notably, the antiinflammatory properties of lingonberries seem to be independent of effects on body weight gain.

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author
organization
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Contribution to journal
publication status
published
subject
keywords
Akkermansia, Berries, Hepatic steatosis, High-fat diet, LBP, Metabolic endotoxemia, Obesity
in
Food & Nutrition Research
volume
60
publisher
Co-action Publishing
external identifiers
  • scopus:84979223171
  • wos:000375881700001
ISSN
1654-6628
DOI
10.3402/fnr.v60.29993
language
English
LU publication?
yes
id
1ee59690-3738-4ec4-b363-6fbed3a9bb2b
date added to LUP
2016-09-08 15:32:05
date last changed
2017-08-13 04:57:52
@article{1ee59690-3738-4ec4-b363-6fbed3a9bb2b,
  abstract     = {<p>Background: The gut microbiota plays an important role in the development of obesity and obesity-associated impairments such as low-grade inflammation. Lingonberries have been shown to prevent diet-induced obesity and low-grade inflammation. However, it is not known whether the effect of lingonberry supplementation is related to modifications of the gut microbiota. The aim of the present study was to describe whether consumption of different batches of lingonberries alters the composition of the gut microbiota, which could be relevant for the protective effect against high fat (HF)-induced metabolic alterations. Methods: Three groups of C57BL/6J mice were fed HF diet with or without a supplement of 20% lingonberries from two different batches (Lingon1 and Lingon2) during 11 weeks. The composition and functionality of the cecal microbiota were assessed by 16S rRNA sequencing and PICRUSt. In addition, parameters related to obesity, insulin sensitivity, hepatic steatosis, inflammation and gut barrier function were examined. Results: HF-induced obesity was only prevented by the Lingon1 diet, whereas both batches of lingonberries reduced plasma levels of markers of inflammation and endotoxemia (SAA and LBP) as well as modified the composition and functionality of the gut microbiota, compared to the HF control group. The relative abundance of Akkermansia and Faecalibacterium, genera associated with healthy gut mucosa and antiinflammation, was found to increase in response to lingonberry intake. Conclusions: Our results show that supplementation with lingonberries to an HF diet prevents low-grade inflammation and is associated with significant changes of the microbiota composition. Notably, the antiinflammatory properties of lingonberries seem to be independent of effects on body weight gain.</p>},
  articleno    = {29993},
  author       = {Heyman Lindén, Lovisa and Kotowska, Dorota and  Sand, Elin and Mikael, Bjursell and Plaza, Merichel and Turner, Charlotta and Holm, Cecilia and Fåk, Frida and Berger, Karin},
  issn         = {1654-6628},
  keyword      = {Akkermansia,Berries,Hepatic steatosis,High-fat diet,LBP,Metabolic endotoxemia,Obesity},
  language     = {eng},
  month        = {04},
  publisher    = {Co-action Publishing},
  series       = {Food & Nutrition Research},
  title        = {Lingonberries alter the gut microbiota and prevent low-grade inflammation in high-fat diet fed mice},
  url          = {http://dx.doi.org/10.3402/fnr.v60.29993},
  volume       = {60},
  year         = {2016},
}