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Co-elution phenomena in polymer mixtures studied by asymmetric flow field-flow fractionation

Zielke, Claudia LU ; Fuentes, Catalina LU ; Piculell, Lennart LU and Nilsson, Lars LU (2018) In Journal of Chromatography A 1532. p.251-256
Abstract

Most polymers generally have complex characteristics. Analysis and understanding of these characteristics is crucial as they, for instance, influence functionality. Separation and analysis of samples of polymers, biopolymers in particular, is challenging since they often display broad distributions in size, structure and molar mass (M) and/or a tendency to form aggregates. Only few analytical techniques are suitable for the task. AF4-MALS-dRI is highly suited for the task, but the analysis can nevertheless be especially challenging for heterogeneous mixtures of polymers that exhibit wide size distributions or aggregation. For such systems, systematic and thorough method development is clearly a requirement. This is the purpose of the... (More)

Most polymers generally have complex characteristics. Analysis and understanding of these characteristics is crucial as they, for instance, influence functionality. Separation and analysis of samples of polymers, biopolymers in particular, is challenging since they often display broad distributions in size, structure and molar mass (M) and/or a tendency to form aggregates. Only few analytical techniques are suitable for the task. AF4-MALS-dRI is highly suited for the task, but the analysis can nevertheless be especially challenging for heterogeneous mixtures of polymers that exhibit wide size distributions or aggregation. For such systems, systematic and thorough method development is clearly a requirement. This is the purpose of the present work, where we approach the problem of heterogeneous polymer samples systematically by analyzing mixtures of two different polymers which are also characterized individually. An often observed phenomenon in AF4 of samples with a high polydispersity is a downturn in M vs. elution time, especially common at high retention. This result is often dismissed as an artifact attributed to various errors in detection and data processing. In this work, we utilize AF4-MALS-dRI to separate and analyze binary mixtures of the well-known polysaccharides pullulan and glycogen, or pullulan and poly(ethylene oxide), respectively, in solution. The results show that an observed downturn – or even an upturn – in M can be a correct result, caused by inherent properties of the analyzed polymers.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Asymmetric flow field-flow fractionation (AF4), Glycogen, Hydrodynamic radius (r), Molar mass (M) distribution, Multi-angle light scattering (MALS), Poly(ethylene) oxide, Pullulan
in
Journal of Chromatography A
volume
1532
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85038414551
ISSN
0021-9673
DOI
10.1016/j.chroma.2017.12.028
language
English
LU publication?
yes
id
210f0104-a0ad-4f13-83de-9d17dff0e01e
date added to LUP
2018-01-03 07:36:59
date last changed
2018-01-04 03:00:13
@article{210f0104-a0ad-4f13-83de-9d17dff0e01e,
  abstract     = {<p>Most polymers generally have complex characteristics. Analysis and understanding of these characteristics is crucial as they, for instance, influence functionality. Separation and analysis of samples of polymers, biopolymers in particular, is challenging since they often display broad distributions in size, structure and molar mass (M) and/or a tendency to form aggregates. Only few analytical techniques are suitable for the task. AF4-MALS-dRI is highly suited for the task, but the analysis can nevertheless be especially challenging for heterogeneous mixtures of polymers that exhibit wide size distributions or aggregation. For such systems, systematic and thorough method development is clearly a requirement. This is the purpose of the present work, where we approach the problem of heterogeneous polymer samples systematically by analyzing mixtures of two different polymers which are also characterized individually. An often observed phenomenon in AF4 of samples with a high polydispersity is a downturn in M vs. elution time, especially common at high retention. This result is often dismissed as an artifact attributed to various errors in detection and data processing. In this work, we utilize AF4-MALS-dRI to separate and analyze binary mixtures of the well-known polysaccharides pullulan and glycogen, or pullulan and poly(ethylene oxide), respectively, in solution. The results show that an observed downturn – or even an upturn – in M can be a correct result, caused by inherent properties of the analyzed polymers.</p>},
  author       = {Zielke, Claudia and Fuentes, Catalina and Piculell, Lennart and Nilsson, Lars},
  issn         = {0021-9673},
  keyword      = {Asymmetric flow field-flow fractionation (AF4),Glycogen,Hydrodynamic radius (r),Molar mass (M) distribution,Multi-angle light scattering (MALS),Poly(ethylene) oxide,Pullulan},
  language     = {eng},
  month        = {01},
  pages        = {251--256},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Co-elution phenomena in polymer mixtures studied by asymmetric flow field-flow fractionation},
  url          = {http://dx.doi.org/10.1016/j.chroma.2017.12.028},
  volume       = {1532},
  year         = {2018},
}