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Efficient induction of functional neurons from adult human fibroblasts.

Pfisterer, Ulrich LU ; Wood, James LU ; Nihlberg, Kristian LU ; Hallgren, Oskar LU ; Bjermer, Leif LU ; Westergren-Thorsson, Gunilla LU ; Lindvall, Olle LU and Parmar, Malin LU (2011) In Cell Cycle 10(19). p.3311-3316
Abstract
Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This... (More)
Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This conversion does not occur through a pluripotent stem cell stage, which eliminates the risk for tumor formation. Here, we demonstrate for the first time that functional neurons can be generated via direct conversion of fibroblasts also from adult individuals. Thus, this technology is an attractive alternative to iPS cells for generating patient- and disease-specific neurons suitable for disease modeling and autologous transplantation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Cycle
volume
10
issue
19
pages
3311 - 3316
publisher
Landes Bioscience
external identifiers
  • wos:000295412100029
  • pmid:21934358
  • scopus:80053446880
ISSN
1551-4005
DOI
10.4161/cc.10.19.17584
language
English
LU publication?
yes
id
de7d93b7-a2da-44ca-8b81-69c48e56504c (old id 2168711)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21934358?dopt=Abstract
date added to LUP
2011-10-03 11:50:10
date last changed
2017-09-24 04:40:12
@article{de7d93b7-a2da-44ca-8b81-69c48e56504c,
  abstract     = {Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This conversion does not occur through a pluripotent stem cell stage, which eliminates the risk for tumor formation. Here, we demonstrate for the first time that functional neurons can be generated via direct conversion of fibroblasts also from adult individuals. Thus, this technology is an attractive alternative to iPS cells for generating patient- and disease-specific neurons suitable for disease modeling and autologous transplantation.},
  author       = {Pfisterer, Ulrich and Wood, James and Nihlberg, Kristian and Hallgren, Oskar and Bjermer, Leif and Westergren-Thorsson, Gunilla and Lindvall, Olle and Parmar, Malin},
  issn         = {1551-4005},
  language     = {eng},
  number       = {19},
  pages        = {3311--3316},
  publisher    = {Landes Bioscience},
  series       = {Cell Cycle},
  title        = {Efficient induction of functional neurons from adult human fibroblasts.},
  url          = {http://dx.doi.org/10.4161/cc.10.19.17584},
  volume       = {10},
  year         = {2011},
}