Efficient induction of functional neurons from adult human fibroblasts.
(2011) In Cell Cycle 10(19). p.3311-3316- Abstract
- Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This... (More)
- Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This conversion does not occur through a pluripotent stem cell stage, which eliminates the risk for tumor formation. Here, we demonstrate for the first time that functional neurons can be generated via direct conversion of fibroblasts also from adult individuals. Thus, this technology is an attractive alternative to iPS cells for generating patient- and disease-specific neurons suitable for disease modeling and autologous transplantation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168711
- author
- Pfisterer, Ulrich LU ; Wood, James LU ; Nihlberg, Kristian LU ; Hallgren, Oskar LU ; Bjermer, Leif LU ; Westergren-Thorsson, Gunilla LU ; Lindvall, Olle LU and Parmar, Malin LU
- organization
-
- Developmental and Regenerative Neurobiology (research group)
- Neurology, Lund
- Lung Biology (research group)
- Department of Experimental Medical Science
- Respiratory Medicine, Allergology, and Palliative Medicine
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- EpiHealth: Epidemiology for Health
- MultiPark: Multidisciplinary research focused on ParkinsonĀ“s disease
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Cycle
- volume
- 10
- issue
- 19
- pages
- 3311 - 3316
- publisher
- Landes Bioscience
- external identifiers
-
- wos:000295412100029
- pmid:21934358
- scopus:80053446880
- pmid:21934358
- ISSN
- 1551-4005
- DOI
- 10.4161/cc.10.19.17584
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Lung Biology (013212002), Respiratory Medicine and Allergology (013230111), Stem Cell Aging (013212073), Neurology, Lund (013027000), Developmental Neurobiology (013210001)
- id
- de7d93b7-a2da-44ca-8b81-69c48e56504c (old id 2168711)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21934358?dopt=Abstract
- date added to LUP
- 2016-04-04 08:55:53
- date last changed
- 2024-06-08 06:24:06
@article{de7d93b7-a2da-44ca-8b81-69c48e56504c, abstract = {{Cellular reprogramming is a rapidly developing technology by which somatic cells are turned into pluripotent stem cells or other somatic cell types through expression of specific combinations of genes. This allows for the generation of patient-specific cell lines that can serve as tools for understanding disease pathogenesis, for drug screens and, potentially, for cell replacement therapies. Several cellular models of neurological disorders based on induced pluripotent cells (iPS cells) have been developed, and iPS-derived neurons are being explored as candidates for transplantation. Recent findings show that neurons can also be induced directly from embryonic and postnatal somatic cells by expression of defined combinations of genes. This conversion does not occur through a pluripotent stem cell stage, which eliminates the risk for tumor formation. Here, we demonstrate for the first time that functional neurons can be generated via direct conversion of fibroblasts also from adult individuals. Thus, this technology is an attractive alternative to iPS cells for generating patient- and disease-specific neurons suitable for disease modeling and autologous transplantation.}}, author = {{Pfisterer, Ulrich and Wood, James and Nihlberg, Kristian and Hallgren, Oskar and Bjermer, Leif and Westergren-Thorsson, Gunilla and Lindvall, Olle and Parmar, Malin}}, issn = {{1551-4005}}, language = {{eng}}, number = {{19}}, pages = {{3311--3316}}, publisher = {{Landes Bioscience}}, series = {{Cell Cycle}}, title = {{Efficient induction of functional neurons from adult human fibroblasts.}}, url = {{http://dx.doi.org/10.4161/cc.10.19.17584}}, doi = {{10.4161/cc.10.19.17584}}, volume = {{10}}, year = {{2011}}, }