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Size, structure and scaling relationships in glycogen from various sources investigated with asymmetrical flow field-flow fractionation and (1)H NMR.

Fernandez, Celine LU ; Rojas, Carola LU and Nilsson, Lars LU (2011) In International Journal of Biological Macromolecules 49(4). p.458-465
Abstract
In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching (1)H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that... (More)
In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching (1)H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that glycogen is a polysaccharide showing rather diverse conformational properties, over the size distribution, depending on its origin and the extraction procedure. The ratio between root-mean-square radius and hydrodynamic radius varies depending of both sample origin the molar mass of the macromolecules, reflecting differences in conformation and scaling within the size distribution. Thus, a priori assumptions regarding the r(rms)/r(h) are difficult to make and r(rms)/r(h) based on average properties give an incomplete description of the properties. Furthermore, the results display the strength of the apparent density (as obtained from AsFlFFF-MALS-RI) as a characterization parameter for scaling in disperse macromolecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Field-flow fractionation, Conformation, Glycogen
in
International Journal of Biological Macromolecules
volume
49
issue
4
pages
458 - 465
publisher
Elsevier
external identifiers
  • wos:000295502500003
  • pmid:21640751
  • scopus:80052275025
ISSN
1879-0003
DOI
10.1016/j.ijbiomac.2011.05.016
language
English
LU publication?
yes
id
00eac4d3-30ab-4162-a005-408ef2e068fd (old id 2208328)
date added to LUP
2011-11-30 12:01:40
date last changed
2017-04-09 03:07:39
@article{00eac4d3-30ab-4162-a005-408ef2e068fd,
  abstract     = {In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching (1)H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that glycogen is a polysaccharide showing rather diverse conformational properties, over the size distribution, depending on its origin and the extraction procedure. The ratio between root-mean-square radius and hydrodynamic radius varies depending of both sample origin the molar mass of the macromolecules, reflecting differences in conformation and scaling within the size distribution. Thus, a priori assumptions regarding the r(rms)/r(h) are difficult to make and r(rms)/r(h) based on average properties give an incomplete description of the properties. Furthermore, the results display the strength of the apparent density (as obtained from AsFlFFF-MALS-RI) as a characterization parameter for scaling in disperse macromolecules.},
  author       = {Fernandez, Celine and Rojas, Carola and Nilsson, Lars},
  issn         = {1879-0003},
  keyword      = {Field-flow fractionation,Conformation,Glycogen},
  language     = {eng},
  number       = {4},
  pages        = {458--465},
  publisher    = {Elsevier},
  series       = {International Journal of Biological Macromolecules},
  title        = {Size, structure and scaling relationships in glycogen from various sources investigated with asymmetrical flow field-flow fractionation and (1)H NMR.},
  url          = {http://dx.doi.org/10.1016/j.ijbiomac.2011.05.016},
  volume       = {49},
  year         = {2011},
}