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Effect of polymorphisms in the leptin, leptin receptor and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition.

Glantz, Maria LU ; Lindmark Månsson, Helena LU ; Stålhammar, Hans and Paulsson, Marie LU (2012) In Journal of Dairy Research 79(1). p.110-118
Abstract
The relations between cow genetics and milk composition have gained a lot of attention during the past years, however, generally only a few compositional traits have been examined. The aim of this study was to determine if polymorphisms in the leptin (LEP), leptin receptor (LEPR) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes as well as genetic polymorphism of β-casein (β-CN), κ-CN and β-lactoglobulin (β-LG) impact several bovine milk composition traits. Individual milk samples from the Swedish Red and Swedish Holstein breeds were analyzed for components in the protein, lipid, carbohydrate and mineral profiles. Cow alleles were determined on the following SNP: A1457G, A252T, A59V and C963T on the LEP gene, T945M on the LEPR... (More)
The relations between cow genetics and milk composition have gained a lot of attention during the past years, however, generally only a few compositional traits have been examined. The aim of this study was to determine if polymorphisms in the leptin (LEP), leptin receptor (LEPR) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes as well as genetic polymorphism of β-casein (β-CN), κ-CN and β-lactoglobulin (β-LG) impact several bovine milk composition traits. Individual milk samples from the Swedish Red and Swedish Holstein breeds were analyzed for components in the protein, lipid, carbohydrate and mineral profiles. Cow alleles were determined on the following SNP: A1457G, A252T, A59V and C963T on the LEP gene, T945M on the LEPR gene and Nt984+8(A-G) on the DGAT1 gene. Additionally, genetic variants of β-CN, κ-CN and β-LG were determined. For both the breeds, the same tendency of minor allele frequency was found for all SNPs and protein genes, except on LEPA1457G and LEPC963T. This study indicated significant (P<0·05) associations between the studied SNPs and several compositional parameters. Protein content was influenced by LEPA1457G (G>A) and LEPC963T (T>C), whereas total Ca, ionic Ca concentration and milk pH were affected by LEPA1457G, LEPA59V, LEPC963T and LEPRT945M. However, yields of milk, protein, CN, lactose, total Ca and P were mainly affected by β-CN (A2>A1) and κ-CN (A>B>E). β-LG was mainly associated with whey protein yield and ionic Ca concentration (A>B). Thus, this study shows possibilities of using these polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acyl-CoA:diacylglycerol, acyltransferase 1 (DGAT1), milk protein polymorphism, milk composition, leptin receptor, Dairy cattle, leptin
in
Journal of Dairy Research
volume
79
issue
1
pages
110 - 118
publisher
Cambridge University Press
external identifiers
  • wos:000300033100015
  • pmid:22127264
  • scopus:84858781795
ISSN
0022-0299
DOI
10.1017/S0022029911000859
language
English
LU publication?
yes
id
58b7971b-f02a-47ae-be18-9207c4b7fd5d (old id 2274802)
date added to LUP
2012-01-12 17:34:23
date last changed
2017-03-26 03:23:22
@article{58b7971b-f02a-47ae-be18-9207c4b7fd5d,
  abstract     = {The relations between cow genetics and milk composition have gained a lot of attention during the past years, however, generally only a few compositional traits have been examined. The aim of this study was to determine if polymorphisms in the leptin (LEP), leptin receptor (LEPR) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes as well as genetic polymorphism of β-casein (β-CN), κ-CN and β-lactoglobulin (β-LG) impact several bovine milk composition traits. Individual milk samples from the Swedish Red and Swedish Holstein breeds were analyzed for components in the protein, lipid, carbohydrate and mineral profiles. Cow alleles were determined on the following SNP: A1457G, A252T, A59V and C963T on the LEP gene, T945M on the LEPR gene and Nt984+8(A-G) on the DGAT1 gene. Additionally, genetic variants of β-CN, κ-CN and β-LG were determined. For both the breeds, the same tendency of minor allele frequency was found for all SNPs and protein genes, except on LEPA1457G and LEPC963T. This study indicated significant (P&lt;0·05) associations between the studied SNPs and several compositional parameters. Protein content was influenced by LEPA1457G (G&gt;A) and LEPC963T (T&gt;C), whereas total Ca, ionic Ca concentration and milk pH were affected by LEPA1457G, LEPA59V, LEPC963T and LEPRT945M. However, yields of milk, protein, CN, lactose, total Ca and P were mainly affected by β-CN (A2&gt;A1) and κ-CN (A&gt;B&gt;E). β-LG was mainly associated with whey protein yield and ionic Ca concentration (A&gt;B). Thus, this study shows possibilities of using these polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters.},
  author       = {Glantz, Maria and Lindmark Månsson, Helena and Stålhammar, Hans and Paulsson, Marie},
  issn         = {0022-0299},
  keyword      = {acyl-CoA:diacylglycerol,acyltransferase 1 (DGAT1),milk protein polymorphism,milk composition,leptin receptor,Dairy cattle,leptin},
  language     = {eng},
  number       = {1},
  pages        = {110--118},
  publisher    = {Cambridge University Press},
  series       = {Journal of Dairy Research},
  title        = {Effect of polymorphisms in the leptin, leptin receptor and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition.},
  url          = {http://dx.doi.org/10.1017/S0022029911000859},
  volume       = {79},
  year         = {2012},
}