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Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells

Chapellier, Marion LU ; Peña-Martínez, Pablo LU ; Ramakrishnan, Ramprasad LU ; Eriksson, Mia LU ; Safaee-Talkhoncheh, Mehrnaz LU ; Orsmark-Pietras, Christina LU ; Lilljebjörn, Henrik LU ; Högberg, Carl LU ; Hagström-Andersson, Anna LU and Fioretos, Thoas LU , et al. (2019) In Haematologica 104(10). p.2006-2016
Abstract

Dysregulation of cytokines in the bone marrow microenvironment promotes acute myeloid leukemia cell growth. Due to the complexity and low throughput of in vivo stem-cell based assays, studying the role of cytokines in the bone marrow niche in a screening setting is challenging. Herein, we developed an ex vivo cytokine screen using 11 arrayed molecular barcodes, allowing for a competitive in vivo readout of leukemia-initiating capacity. With this approach, we assessed the effect of 114 murine cytokines on MLL-AF9 acute myeloid leukemia mouse cells and identified the tumor necrosis factor ligand superfamily member 13 (TNFSF13) as a positive regulator of leukemia-initiating cells. By using Tnfsf13-/- recipient mice, we confirmed that... (More)

Dysregulation of cytokines in the bone marrow microenvironment promotes acute myeloid leukemia cell growth. Due to the complexity and low throughput of in vivo stem-cell based assays, studying the role of cytokines in the bone marrow niche in a screening setting is challenging. Herein, we developed an ex vivo cytokine screen using 11 arrayed molecular barcodes, allowing for a competitive in vivo readout of leukemia-initiating capacity. With this approach, we assessed the effect of 114 murine cytokines on MLL-AF9 acute myeloid leukemia mouse cells and identified the tumor necrosis factor ligand superfamily member 13 (TNFSF13) as a positive regulator of leukemia-initiating cells. By using Tnfsf13-/- recipient mice, we confirmed that TNFSF13 supports leukemia-initiation also under physiological conditions. TNFSF13 was secreted by normal myeloid cells but not by leukemia mouse cells, suggesting that mature myeloid bone marrow cells support leukemia cells by secreting TNFSF13. TNFSF13 supported leukemia cell proliferation in an NF-κB-dependent manner by binding TNFRSF17 and suppressed apoptosis. Moreover, TNFSF13 supported the growth and survival of several human myeloid leukemia cell lines, demonstrating that our findings translate to human disease. Taken together, using arrayed molecular barcoding, we identified a previously unrecognized role of TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells. The arrayed barcoded screening methodology is not limited to cytokines and leukemia, but can be extended to other types of ex vivo screens, where a multiplexed in vivo read-out of stem cell functionality is needed.

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Contribution to journal
publication status
published
subject
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Haematologica
volume
104
issue
10
pages
2006 - 2016
publisher
Ferrata Storti Foundation
external identifiers
  • scopus:85072847686
  • pmid:30819903
ISSN
1592-8721
DOI
10.3324/haematol.2018.192062
language
English
LU publication?
yes
additional info
Copyright © 2019, Ferrata Storti Foundation.
id
3209458b-88ff-417f-8255-5319192c4862
date added to LUP
2019-06-19 14:02:36
date last changed
2020-01-13 02:03:28
@article{3209458b-88ff-417f-8255-5319192c4862,
  abstract     = {<p>Dysregulation of cytokines in the bone marrow microenvironment promotes acute myeloid leukemia cell growth. Due to the complexity and low throughput of in vivo stem-cell based assays, studying the role of cytokines in the bone marrow niche in a screening setting is challenging. Herein, we developed an ex vivo cytokine screen using 11 arrayed molecular barcodes, allowing for a competitive in vivo readout of leukemia-initiating capacity. With this approach, we assessed the effect of 114 murine cytokines on MLL-AF9 acute myeloid leukemia mouse cells and identified the tumor necrosis factor ligand superfamily member 13 (TNFSF13) as a positive regulator of leukemia-initiating cells. By using Tnfsf13-/- recipient mice, we confirmed that TNFSF13 supports leukemia-initiation also under physiological conditions. TNFSF13 was secreted by normal myeloid cells but not by leukemia mouse cells, suggesting that mature myeloid bone marrow cells support leukemia cells by secreting TNFSF13. TNFSF13 supported leukemia cell proliferation in an NF-κB-dependent manner by binding TNFRSF17 and suppressed apoptosis. Moreover, TNFSF13 supported the growth and survival of several human myeloid leukemia cell lines, demonstrating that our findings translate to human disease. Taken together, using arrayed molecular barcoding, we identified a previously unrecognized role of TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells. The arrayed barcoded screening methodology is not limited to cytokines and leukemia, but can be extended to other types of ex vivo screens, where a multiplexed in vivo read-out of stem cell functionality is needed.</p>},
  author       = {Chapellier, Marion and Peña-Martínez, Pablo and Ramakrishnan, Ramprasad and Eriksson, Mia and Safaee-Talkhoncheh, Mehrnaz and Orsmark-Pietras, Christina and Lilljebjörn, Henrik and Högberg, Carl and Hagström-Andersson, Anna and Fioretos, Thoas and Larsson, Jonas and Järås, Marcus},
  issn         = {1592-8721},
  language     = {eng},
  number       = {10},
  pages        = {2006--2016},
  publisher    = {Ferrata Storti Foundation},
  series       = {Haematologica},
  title        = {Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells},
  url          = {http://dx.doi.org/10.3324/haematol.2018.192062},
  doi          = {10.3324/haematol.2018.192062},
  volume       = {104},
  year         = {2019},
}