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Plasma enterostatin: Identification and release in rats in response to a meal

Mei, Jie LU ; Sörhede Winzell, Maria LU and Erlanson-Albertsson, Charlotte LU (2002) In Obesity Research 10(7). p.688-694
Abstract
Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels... (More)
Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fat intake, procolipase, appetite, sucrose, enzyme-linked immunosorbent, assay
in
Obesity Research
volume
10
issue
7
pages
688 - 694
publisher
The North American Association for the Study of Obesity
external identifiers
  • wos:000176860900016
  • pmid:12105292
  • scopus:0036636781
ISSN
1071-7323
language
English
LU publication?
yes
id
6eef3a62-affd-48f9-8e7a-d13860de800f (old id 333235)
alternative location
http://www.obesityresearch.org/cgi/reprint/10/7/688
date added to LUP
2007-11-16 13:36:25
date last changed
2017-01-01 06:44:54
@article{6eef3a62-affd-48f9-8e7a-d13860de800f,
  abstract     = {Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake.},
  author       = {Mei, Jie and Sörhede Winzell, Maria and Erlanson-Albertsson, Charlotte},
  issn         = {1071-7323},
  keyword      = {fat intake,procolipase,appetite,sucrose,enzyme-linked immunosorbent,assay},
  language     = {eng},
  number       = {7},
  pages        = {688--694},
  publisher    = {The North American Association for the Study of Obesity},
  series       = {Obesity Research},
  title        = {Plasma enterostatin: Identification and release in rats in response to a meal},
  volume       = {10},
  year         = {2002},
}