Plasma enterostatin: Identification and release in rats in response to a meal
(2002) In Obesity Research 10(7). p.688-694- Abstract
- Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels... (More)
- Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/333235
- author
- Mei, Jie LU ; Sörhede Winzell, Maria LU and Erlanson-Albertsson, Charlotte LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fat intake, procolipase, appetite, sucrose, enzyme-linked immunosorbent, assay
- in
- Obesity Research
- volume
- 10
- issue
- 7
- pages
- 688 - 694
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000176860900016
- pmid:12105292
- scopus:0036636781
- ISSN
- 1071-7323
- language
- English
- LU publication?
- yes
- id
- 6eef3a62-affd-48f9-8e7a-d13860de800f (old id 333235)
- alternative location
- http://www.obesityresearch.org/cgi/reprint/10/7/688
- date added to LUP
- 2016-04-01 15:42:01
- date last changed
- 2024-02-26 06:30:57
@article{6eef3a62-affd-48f9-8e7a-d13860de800f,
abstract = {{Objective: To discover a possible absorption and/or secretion of enterostatin into the circulating blood, as well as to compare the levels of circulating enterostatin after high-fat feeding and low-fat feeding. Research Methods and Procedures: Using a specific enzyme-linked immunosorbent assay, plasma enterostatin levels were determined after feeding a high-fat, a high-fat/sucrose, or a low-fat meal to Sprague-Dawley rats deprived of food overnight. Results: The enterostatin levels were increased by all diets; the response to the high-fat and the high-fat/-sucrose meals was greater in magnitude and duration than that to the low-fat meal. In addition, enterostatin levels correlated with the intake of dietary fat. Plasma enterostatin levels after high-fat feeding were found to be similar to those after intravenous administration of exogenous enterostatin known to inhibit high-fat food intake. Gel chromatography of pooled postprandial plasma extracts followed by high-performance liquid chromatography analysis showed that plasma enterostatin was identical to synthetic enterostatin. Affinity cross-linking of plasma proteins with I-125-enterostatin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography, revealed a single band with a molecular weight of about 66 kDa, indicating the presence of a potential enterostatin-binding protein in plasma. Discussion: The measurements of plasma enterostatin may be a sensitive indicator for the measurement of fat intake.}},
author = {{Mei, Jie and Sörhede Winzell, Maria and Erlanson-Albertsson, Charlotte}},
issn = {{1071-7323}},
keywords = {{fat intake; procolipase; appetite; sucrose; enzyme-linked immunosorbent; assay}},
language = {{eng}},
number = {{7}},
pages = {{688--694}},
publisher = {{Nature Publishing Group}},
series = {{Obesity Research}},
title = {{Plasma enterostatin: Identification and release in rats in response to a meal}},
url = {{http://www.obesityresearch.org/cgi/reprint/10/7/688}},
volume = {{10}},
year = {{2002}},
}