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On-line capillary based quantitative analysis of a heterocyclic amine in human urine

Viberg, Peter LU ; Wahlund, Karl-Gustav LU and Skog, Kerstin LU (2006) In Journal of Chromatography A 1133(1-2). p.347-352
Abstract
A high through-put miniaturised separation-quantification method for the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine was developed. The limit of detection was of 0.65 fmol (0.14 pg) injected or 65 pM. Heterocyclic aromatic amines are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations and the high complexity of the matrix in which they exist (food, blood, and urine), and the often small sample volumes (capillary blood; urine, blood and milk from small animals), very sensitive and selective analytical methods are required for their detection. Miniaturisation was accomplished by micro solid-phase extraction in capillaries... (More)
A high through-put miniaturised separation-quantification method for the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine was developed. The limit of detection was of 0.65 fmol (0.14 pg) injected or 65 pM. Heterocyclic aromatic amines are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations and the high complexity of the matrix in which they exist (food, blood, and urine), and the often small sample volumes (capillary blood; urine, blood and milk from small animals), very sensitive and selective analytical methods are required for their detection. Miniaturisation was accomplished by micro solid-phase extraction in capillaries with blue chitin as solid-phase, coupled on-line (in-capillary) to capillary electrophoresis with nanospray tandem mass spectrometric detection. This new technique provided a total analysis time of only 15 min, including extraction and separation, together with low sample and solvent consumption. Blue chitin showed high tolerance towards the urine matrix. Urine collected 12 h after consumption of fried chicken contained 1.8 nmol L-1 (0.40 pg mu L-1) of PhIP. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PhIP, blue chitin, capillary electrophoresis, spectrometry, tandem mass, nanoelectrospray, nanospray, on-line, in-capillary, food analysis, Heterocyclic amines
in
Journal of Chromatography A
volume
1133
issue
1-2
pages
347 - 352
publisher
Elsevier
external identifiers
  • wos:000241772800040
  • scopus:33749994327
ISSN
0021-9673
DOI
10.1016/j.chroma.2006.08.034
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Applied Nutrition and Food Chemistry (011001300)
id
5b5ab237-c457-411e-a535-93070407869f (old id 378363)
date added to LUP
2016-04-01 15:27:50
date last changed
2021-02-17 04:54:09
@article{5b5ab237-c457-411e-a535-93070407869f,
  abstract     = {A high through-put miniaturised separation-quantification method for the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine was developed. The limit of detection was of 0.65 fmol (0.14 pg) injected or 65 pM. Heterocyclic aromatic amines are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations and the high complexity of the matrix in which they exist (food, blood, and urine), and the often small sample volumes (capillary blood; urine, blood and milk from small animals), very sensitive and selective analytical methods are required for their detection. Miniaturisation was accomplished by micro solid-phase extraction in capillaries with blue chitin as solid-phase, coupled on-line (in-capillary) to capillary electrophoresis with nanospray tandem mass spectrometric detection. This new technique provided a total analysis time of only 15 min, including extraction and separation, together with low sample and solvent consumption. Blue chitin showed high tolerance towards the urine matrix. Urine collected 12 h after consumption of fried chicken contained 1.8 nmol L-1 (0.40 pg mu L-1) of PhIP. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Viberg, Peter and Wahlund, Karl-Gustav and Skog, Kerstin},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {347--352},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {On-line capillary based quantitative analysis of a heterocyclic amine in human urine},
  url          = {http://dx.doi.org/10.1016/j.chroma.2006.08.034},
  doi          = {10.1016/j.chroma.2006.08.034},
  volume       = {1133},
  year         = {2006},
}