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Analysis of β-glucan molar mass from barley malt and brewer's spent grain with asymmetric flow field-flow fractionation (AF4) and their association to proteins

Zielke, Claudia LU ; Teixeira, Cristina LU ; Ding, Huihuang; Cui, Steve; Nyman, Margareta LU and Nilsson, Lars LU (2017) In Carbohydrate Polymers 157. p.541-549
Abstract

β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, <2000 to approximately 6.7 × 106 g/mol. Differences in molar masses were more noticeable... (More)

β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, <2000 to approximately 6.7 × 106 g/mol. Differences in molar masses were more noticeable between barley varieties and steeping malting conditions than by mashing of malt. Barley products processed to preserve β-glucan contained more β-glucan of high molar mass with potential to shift the fermentation site to the distal colon. Enzymatic degradation of proteins indicated presence of aggregates containing β-glucan and protein.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Asymmetric flow field-flow fractionation (AF4), Calcofluor, Colonic health, Multiangle light scattering (MALS), Protein-carbohydrate complexes
in
Carbohydrate Polymers
volume
157
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:84992428121
  • wos:000391896800061
ISSN
0144-8617
DOI
10.1016/j.carbpol.2016.10.045
language
English
LU publication?
yes
id
436fabc5-133b-4d54-8fd5-2cce7c0231d4
date added to LUP
2017-02-03 10:25:38
date last changed
2018-01-07 11:47:56
@article{436fabc5-133b-4d54-8fd5-2cce7c0231d4,
  abstract     = {<p>β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, &lt;2000 to approximately 6.7 × 10<sup>6</sup> g/mol. Differences in molar masses were more noticeable between barley varieties and steeping malting conditions than by mashing of malt. Barley products processed to preserve β-glucan contained more β-glucan of high molar mass with potential to shift the fermentation site to the distal colon. Enzymatic degradation of proteins indicated presence of aggregates containing β-glucan and protein.</p>},
  author       = {Zielke, Claudia and Teixeira, Cristina and Ding, Huihuang and Cui, Steve and Nyman, Margareta and Nilsson, Lars},
  issn         = {0144-8617},
  keyword      = {Asymmetric flow field-flow fractionation (AF4),Calcofluor,Colonic health,Multiangle light scattering (MALS),Protein-carbohydrate complexes},
  language     = {eng},
  month        = {02},
  pages        = {541--549},
  publisher    = {Elsevier},
  series       = {Carbohydrate Polymers},
  title        = {Analysis of β-glucan molar mass from barley malt and brewer's spent grain with asymmetric flow field-flow fractionation (AF4) and their association to proteins},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2016.10.045},
  volume       = {157},
  year         = {2017},
}