Analysis of β-glucan molar mass from barley malt and brewer's spent grain with asymmetric flow field-flow fractionation (AF4) and their association to proteins
(2017) In Carbohydrate Polymers 157. p.541-549- Abstract
β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, <2000 to approximately 6.7 × 106 g/mol. Differences in molar masses were more noticeable... (More)
β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, <2000 to approximately 6.7 × 106 g/mol. Differences in molar masses were more noticeable between barley varieties and steeping malting conditions than by mashing of malt. Barley products processed to preserve β-glucan contained more β-glucan of high molar mass with potential to shift the fermentation site to the distal colon. Enzymatic degradation of proteins indicated presence of aggregates containing β-glucan and protein.
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- author
- Zielke, Claudia LU ; Teixeira, Cristina LU ; Ding, Huihuang ; Cui, Steve ; Nyman, Margareta LU and Nilsson, Lars LU
- organization
- publishing date
- 2017-02-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Asymmetric flow field-flow fractionation (AF4), Calcofluor, Colonic health, Multiangle light scattering (MALS), Protein-carbohydrate complexes
- in
- Carbohydrate Polymers
- volume
- 157
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27987960
- wos:000391896800061
- scopus:84992428121
- ISSN
- 0144-8617
- DOI
- 10.1016/j.carbpol.2016.10.045
- language
- English
- LU publication?
- yes
- id
- 436fabc5-133b-4d54-8fd5-2cce7c0231d4
- date added to LUP
- 2017-02-03 10:25:38
- date last changed
- 2024-03-30 23:34:23
@article{436fabc5-133b-4d54-8fd5-2cce7c0231d4,
abstract = {{<p>β-Glucan benefits are related with its molar mass and it would be of interest to better understand how this parameter can be changed by processing and variety for design of food with specific health effects. For this purpose, extracts from barley malts and brewers' spent grain, processed at different conditions, were analysed regarding β-glucan content, molar mass, and protein content. Molar mass distribution was assessed using asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection. β-Glucan was detected in a wide molar mass range, <2000 to approximately 6.7 × 10<sup>6</sup> g/mol. Differences in molar masses were more noticeable between barley varieties and steeping malting conditions than by mashing of malt. Barley products processed to preserve β-glucan contained more β-glucan of high molar mass with potential to shift the fermentation site to the distal colon. Enzymatic degradation of proteins indicated presence of aggregates containing β-glucan and protein.</p>}},
author = {{Zielke, Claudia and Teixeira, Cristina and Ding, Huihuang and Cui, Steve and Nyman, Margareta and Nilsson, Lars}},
issn = {{0144-8617}},
keywords = {{Asymmetric flow field-flow fractionation (AF4); Calcofluor; Colonic health; Multiangle light scattering (MALS); Protein-carbohydrate complexes}},
language = {{eng}},
month = {{02}},
pages = {{541--549}},
publisher = {{Elsevier}},
series = {{Carbohydrate Polymers}},
title = {{Analysis of β-glucan molar mass from barley malt and brewer's spent grain with asymmetric flow field-flow fractionation (AF4) and their association to proteins}},
url = {{http://dx.doi.org/10.1016/j.carbpol.2016.10.045}},
doi = {{10.1016/j.carbpol.2016.10.045}},
volume = {{157}},
year = {{2017}},
}