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Actin-like protein associated with plasma membranes from Euglena gracilis

Petersen-Mahrt, Silja ; Sonesson, Anders LU and Widell, Susanne LU (1998) In Protoplasma 202(3-4). p.153-160
Abstract
Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex... (More)
Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin. (Less)
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; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Actin, Anti-actin antibody, Cytoskeleton, Euglena gracilis, Triton X-114, DNAse I
in
Protoplasma
volume
202
issue
3-4
pages
8 pages
publisher
Springer
external identifiers
  • scopus:0031839279
ISSN
1615-6102
DOI
10.1007/BF01282543
language
English
LU publication?
yes
additional info
Department affilation moved from v1000887 (CED - Centre for Educational Development) to v1000942 (Division for Higher Education Development) on 2016-03-31 08:48:47.
id
c80c4c40-206a-4a63-a408-de39707c7cf7 (old id 4438048)
date added to LUP
2016-04-04 08:12:36
date last changed
2022-01-29 03:10:36
@article{c80c4c40-206a-4a63-a408-de39707c7cf7,
  abstract     = {{Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin.}},
  author       = {{Petersen-Mahrt, Silja and Sonesson, Anders and Widell, Susanne}},
  issn         = {{1615-6102}},
  keywords     = {{Actin; Anti-actin antibody; Cytoskeleton; Euglena gracilis; Triton X-114; DNAse I}},
  language     = {{eng}},
  number       = {{3-4}},
  pages        = {{153--160}},
  publisher    = {{Springer}},
  series       = {{Protoplasma}},
  title        = {{Actin-like protein associated with plasma membranes from Euglena gracilis}},
  url          = {{http://dx.doi.org/10.1007/BF01282543}},
  doi          = {{10.1007/BF01282543}},
  volume       = {{202}},
  year         = {{1998}},
}