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Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants

Sonesson, Anders LU and Widell, Susanne LU (1993) In Protoplasma 177(1-2). p.45-52
Abstract
Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well... (More)
Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Actin, Cauliflower, Cortical cytoskeleton, Membrane cytoskeleton, Plant plasma membrane.
in
Protoplasma
volume
177
issue
1-2
pages
45 - 52
publisher
Springer
external identifiers
  • scopus:0027133390
ISSN
1615-6102
DOI
10.1007/BF01403398
language
English
LU publication?
yes
id
b86eb1ee-4150-479d-85a9-824c090e1ef8 (old id 4438065)
alternative location
http://dx.doi.org/10.1007/BF01403398
date added to LUP
2014-05-21 11:43:24
date last changed
2017-07-16 04:11:00
@article{b86eb1ee-4150-479d-85a9-824c090e1ef8,
  abstract     = {Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.},
  author       = {Sonesson, Anders and Widell, Susanne},
  issn         = {1615-6102},
  keyword      = {Actin,Cauliflower,Cortical cytoskeleton,Membrane cytoskeleton,Plant plasma membrane.},
  language     = {eng},
  number       = {1-2},
  pages        = {45--52},
  publisher    = {Springer},
  series       = {Protoplasma},
  title        = {Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants},
  url          = {http://dx.doi.org/10.1007/BF01403398},
  volume       = {177},
  year         = {1993},
}