Brain pericytes acquire a microglial phenotype after stroke.
(2014) In Acta Neuropathologica 128(3). p.381-396- Abstract
- Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human... (More)
- Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human stroke brain tissue. We demonstrate that human brain-derived pericytes adopt a microglial phenotype and upregulate mRNA specific for activated microglial cells under hypoxic conditions in vitro. Our study indicates that the vasculature is a novel source of inflammatory cells with a microglial phenotype in brain ischemia and hence identifies pericytes as an important new target for the development of future stroke therapies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4453976
- author
- Ozen, Ilknur LU ; Deierborg, Tomas LU ; Miharada, Kenichi LU ; Padel, Thomas LU ; Englund, Elisabet LU ; Genové, Guillem and Paul-Visse, Gesine LU
- organization
-
- Neurology, Lund
- Neuroinflammation (research group)
- Division of Molecular Medicine and Gene Therapy
- Stem Cell Metabolism (research group)
- Tumor microenvironment
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Neuropathologica
- volume
- 128
- issue
- 3
- pages
- 381 - 396
- publisher
- Springer
- external identifiers
-
- pmid:24848101
- wos:000340551900006
- scopus:84906319162
- pmid:24848101
- ISSN
- 1432-0533
- DOI
- 10.1007/s00401-014-1295-x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Pathology, (Lund) (013030000), Division of Molecular Medicine and Gene Therapy (013022010), Neuroinflammation (013210006)
- id
- ce59eb0c-b82b-4e1a-9389-4ca08e96a177 (old id 4453976)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24848101?dopt=Abstract
- date added to LUP
- 2016-04-01 10:07:09
- date last changed
- 2022-05-17 19:58:17
@article{ce59eb0c-b82b-4e1a-9389-4ca08e96a177, abstract = {{Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human stroke brain tissue. We demonstrate that human brain-derived pericytes adopt a microglial phenotype and upregulate mRNA specific for activated microglial cells under hypoxic conditions in vitro. Our study indicates that the vasculature is a novel source of inflammatory cells with a microglial phenotype in brain ischemia and hence identifies pericytes as an important new target for the development of future stroke therapies.}}, author = {{Ozen, Ilknur and Deierborg, Tomas and Miharada, Kenichi and Padel, Thomas and Englund, Elisabet and Genové, Guillem and Paul-Visse, Gesine}}, issn = {{1432-0533}}, language = {{eng}}, number = {{3}}, pages = {{381--396}}, publisher = {{Springer}}, series = {{Acta Neuropathologica}}, title = {{Brain pericytes acquire a microglial phenotype after stroke.}}, url = {{http://dx.doi.org/10.1007/s00401-014-1295-x}}, doi = {{10.1007/s00401-014-1295-x}}, volume = {{128}}, year = {{2014}}, }