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Brain pericytes acquire a microglial phenotype after stroke.

Ozen, Ilknur LU ; Deierborg, Tomas LU ; Miharada, Kenichi LU ; Padel, Thomas LU ; Englund, Elisabet LU ; Genové, Guillem and Paul-Visse, Gesine LU (2014) In Acta Neuropathologica 128(3). p.381-396
Abstract
Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human... (More)
Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human stroke brain tissue. We demonstrate that human brain-derived pericytes adopt a microglial phenotype and upregulate mRNA specific for activated microglial cells under hypoxic conditions in vitro. Our study indicates that the vasculature is a novel source of inflammatory cells with a microglial phenotype in brain ischemia and hence identifies pericytes as an important new target for the development of future stroke therapies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Neuropathologica
volume
128
issue
3
pages
381 - 396
publisher
Springer
external identifiers
  • pmid:24848101
  • wos:000340551900006
  • scopus:84906319162
ISSN
1432-0533
DOI
10.1007/s00401-014-1295-x
language
English
LU publication?
yes
id
ce59eb0c-b82b-4e1a-9389-4ca08e96a177 (old id 4453976)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24848101?dopt=Abstract
date added to LUP
2014-06-04 22:40:46
date last changed
2017-10-29 03:05:46
@article{ce59eb0c-b82b-4e1a-9389-4ca08e96a177,
  abstract     = {Pericytes are located on the abluminal side of endothelial cells lining the microvasculature in all organs. They have been identified as multipotent progenitor cells in several tissues of the body including the human brain. New evidence suggests that pericytes contribute to tissue repair, but their role in the injured brain is largely unknown. Here, we investigate the role of pericytes in ischemic stroke. Using a pericyte-reporter mouse model, we provide unique evidence that regulator of G-protein signaling 5 expressing cells are activated pericytes that leave the blood vessel wall, proliferate and give rise to microglial cells after ischemic brain injury. Consistently, we show that activated pericytes express microglial markers in human stroke brain tissue. We demonstrate that human brain-derived pericytes adopt a microglial phenotype and upregulate mRNA specific for activated microglial cells under hypoxic conditions in vitro. Our study indicates that the vasculature is a novel source of inflammatory cells with a microglial phenotype in brain ischemia and hence identifies pericytes as an important new target for the development of future stroke therapies.},
  author       = {Ozen, Ilknur and Deierborg, Tomas and Miharada, Kenichi and Padel, Thomas and Englund, Elisabet and Genové, Guillem and Paul-Visse, Gesine},
  issn         = {1432-0533},
  language     = {eng},
  number       = {3},
  pages        = {381--396},
  publisher    = {Springer},
  series       = {Acta Neuropathologica},
  title        = {Brain pericytes acquire a microglial phenotype after stroke.},
  url          = {http://dx.doi.org/10.1007/s00401-014-1295-x},
  volume       = {128},
  year         = {2014},
}