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Analysis of polysaccharide and proteinaceous macromolecules in beer using asymmetrical flow field-flow fractionation

Tugel, Isilay; Runyon, Ray LU ; Gomez, Federico LU and Nilsson, Lars LU (2015) In Journal of the Institute of Brewing 121(1). p.44-48
Abstract
This paper demonstrates the potential of asymmetrical flow field flow fractionation coupled with online multi-angle light scattering, differential refractive index and UV detection for the fractionation and analysis of macromolecules in beer regarding their composition, molar mass (M) and relative concentration. The macromolecules in the liquid and foam of two types of beer, light lager and porter, were analysed in their native state with minimal sample preparation. The results showed the presence of three major populations of macromolecules. In lager beer liquid, the early eluting population has an average M of 2x10(4)g/mol and an intense UV absorbance at 280nm suggesting the presence of proteinaceous macromolecules. The second and the... (More)
This paper demonstrates the potential of asymmetrical flow field flow fractionation coupled with online multi-angle light scattering, differential refractive index and UV detection for the fractionation and analysis of macromolecules in beer regarding their composition, molar mass (M) and relative concentration. The macromolecules in the liquid and foam of two types of beer, light lager and porter, were analysed in their native state with minimal sample preparation. The results showed the presence of three major populations of macromolecules. In lager beer liquid, the early eluting population has an average M of 2x10(4)g/mol and an intense UV absorbance at 280nm suggesting the presence of proteinaceous macromolecules. The second and the third populations, which elute at consecutively longer retention times, have M ranging from 10(5) to 10(7)g/mol. They are not UV-active at 280nm, suggesting the elution of polysaccharides. The second population was identified as -glucans as a result of -glucanase treatment. The third population was not identified in the present study. The results show that similar populations are present in lager beer foam and that the macromolecules appear to be present in a more aggregated state. The M range of macromolecules in porter beer liquid ranged from 10(5) to 10(8)g/mol. A fraction of macromolecules eluting at longer retention times is highly UV-active, which shows that there are great variations in the macromolecular profile of lager and porter beer. Copyright (c) 2015 The Institute of Brewing & Distilling (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beer, macromolecules, protein aggregation, polysaccharide, beta-glucan, asymmetrical flow field-flow fractionation
in
Journal of the Institute of Brewing
volume
121
issue
1
pages
44 - 48
publisher
Inst Brewing, Wiley
external identifiers
  • wos:000350276100006
  • scopus:84923095387
ISSN
0046-9750
DOI
10.1002/jib.195
language
English
LU publication?
yes
id
72ad0f31-4791-4080-8f38-d5bd28d63891 (old id 5304040)
date added to LUP
2015-04-24 11:31:26
date last changed
2017-11-05 03:26:08
@article{72ad0f31-4791-4080-8f38-d5bd28d63891,
  abstract     = {This paper demonstrates the potential of asymmetrical flow field flow fractionation coupled with online multi-angle light scattering, differential refractive index and UV detection for the fractionation and analysis of macromolecules in beer regarding their composition, molar mass (M) and relative concentration. The macromolecules in the liquid and foam of two types of beer, light lager and porter, were analysed in their native state with minimal sample preparation. The results showed the presence of three major populations of macromolecules. In lager beer liquid, the early eluting population has an average M of 2x10(4)g/mol and an intense UV absorbance at 280nm suggesting the presence of proteinaceous macromolecules. The second and the third populations, which elute at consecutively longer retention times, have M ranging from 10(5) to 10(7)g/mol. They are not UV-active at 280nm, suggesting the elution of polysaccharides. The second population was identified as -glucans as a result of -glucanase treatment. The third population was not identified in the present study. The results show that similar populations are present in lager beer foam and that the macromolecules appear to be present in a more aggregated state. The M range of macromolecules in porter beer liquid ranged from 10(5) to 10(8)g/mol. A fraction of macromolecules eluting at longer retention times is highly UV-active, which shows that there are great variations in the macromolecular profile of lager and porter beer. Copyright (c) 2015 The Institute of Brewing & Distilling},
  author       = {Tugel, Isilay and Runyon, Ray and Gomez, Federico and Nilsson, Lars},
  issn         = {0046-9750},
  keyword      = {beer,macromolecules,protein aggregation,polysaccharide,beta-glucan,asymmetrical flow field-flow fractionation},
  language     = {eng},
  number       = {1},
  pages        = {44--48},
  publisher    = {Inst Brewing, Wiley},
  series       = {Journal of the Institute of Brewing},
  title        = {Analysis of polysaccharide and proteinaceous macromolecules in beer using asymmetrical flow field-flow fractionation},
  url          = {http://dx.doi.org/10.1002/jib.195},
  volume       = {121},
  year         = {2015},
}