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Xylooligosaccharides from Hardwood and Cereal Xylans Produced by a Thermostable Xylanase as Carbon Sources for Lactobacillus brevis and Bifidobacterium adolescentis.

Falck, Peter LU ; Precha-Atsawanan, Suthsiri ; Grey, Carl LU ; Immerzeel, Peter LU ; Stålbrand, Henrik LU ; Adlercreutz, Patrick LU and Nordberg Karlsson, Eva LU (2013) In Journal of Agricultural and Food Chemistry 61(30). p.7333-7340
Abstract
To compare xylans from forestry with agricultural origins, hardwood xylan (birch) and cereal arabinoxylan (rye) were hydrolyzed using two variants of the xylanase RmXyn10A, full-length enzyme and catalytic module only, from Rhodothermus marinus . Cultivations of four selected bacterial species, using the xylooligosaccharide (XOS) containing hydrolysates as carbon source, showed selective growth of Lactobacillus brevis DSMZ 1264 and Bifidobacterium adolescentis ATCC 15703. Both strains were confirmed to utilize the XOS fraction (DP 2-5), whereas putative arabinoxylooligosaccharides from the rye arabinoxylan hydrolysate were utilized by only B. adolescentis. Escherichia coli did not grow, despite its capability to grow on the monosaccharides... (More)
To compare xylans from forestry with agricultural origins, hardwood xylan (birch) and cereal arabinoxylan (rye) were hydrolyzed using two variants of the xylanase RmXyn10A, full-length enzyme and catalytic module only, from Rhodothermus marinus . Cultivations of four selected bacterial species, using the xylooligosaccharide (XOS) containing hydrolysates as carbon source, showed selective growth of Lactobacillus brevis DSMZ 1264 and Bifidobacterium adolescentis ATCC 15703. Both strains were confirmed to utilize the XOS fraction (DP 2-5), whereas putative arabinoxylooligosaccharides from the rye arabinoxylan hydrolysate were utilized by only B. adolescentis. Escherichia coli did not grow, despite its capability to grow on the monosaccharides arabinose and xylose. It was also shown that Pediococcus parvulus strain 2.6 utilized neither xylose nor XOS for growth. In summary, RmXyn10A or its catalytic module proved suitable for high-temperature hydrolysis of hardwood xylan and cereal arabinoxylan, producing XOS that could qualify as prebiotics for use in functional food products. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Agricultural and Food Chemistry
volume
61
issue
30
pages
7333 - 7340
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000322752700021
  • pmid:23822770
  • scopus:84881055177
ISSN
0021-8561
DOI
10.1021/jf401249g
project
ANTIDIABETIC FOOD CENTRE
language
English
LU publication?
yes
id
56a1a669-84b4-4b24-bcaf-e219bd3d5c36 (old id 3956265)
date added to LUP
2016-04-01 11:14:29
date last changed
2020-02-26 02:49:07
@article{56a1a669-84b4-4b24-bcaf-e219bd3d5c36,
  abstract     = {To compare xylans from forestry with agricultural origins, hardwood xylan (birch) and cereal arabinoxylan (rye) were hydrolyzed using two variants of the xylanase RmXyn10A, full-length enzyme and catalytic module only, from Rhodothermus marinus . Cultivations of four selected bacterial species, using the xylooligosaccharide (XOS) containing hydrolysates as carbon source, showed selective growth of Lactobacillus brevis DSMZ 1264 and Bifidobacterium adolescentis ATCC 15703. Both strains were confirmed to utilize the XOS fraction (DP 2-5), whereas putative arabinoxylooligosaccharides from the rye arabinoxylan hydrolysate were utilized by only B. adolescentis. Escherichia coli did not grow, despite its capability to grow on the monosaccharides arabinose and xylose. It was also shown that Pediococcus parvulus strain 2.6 utilized neither xylose nor XOS for growth. In summary, RmXyn10A or its catalytic module proved suitable for high-temperature hydrolysis of hardwood xylan and cereal arabinoxylan, producing XOS that could qualify as prebiotics for use in functional food products.},
  author       = {Falck, Peter and Precha-Atsawanan, Suthsiri and Grey, Carl and Immerzeel, Peter and Stålbrand, Henrik and Adlercreutz, Patrick and Nordberg Karlsson, Eva},
  issn         = {0021-8561},
  language     = {eng},
  number       = {30},
  pages        = {7333--7340},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Xylooligosaccharides from Hardwood and Cereal Xylans Produced by a Thermostable Xylanase as Carbon Sources for Lactobacillus brevis and Bifidobacterium adolescentis.},
  url          = {http://dx.doi.org/10.1021/jf401249g},
  doi          = {10.1021/jf401249g},
  volume       = {61},
  year         = {2013},
}