Advanced

Consequences of metabolic challenges on hypothalamic colipase and PLRP2 mRNA in rats.

Rippe, Catarina LU ; Erlanson-Albertsson, Charlotte LU and Lindqvist, Andreas LU (2007) In Brain Research 1185. p.152-157
Abstract
The hypothalamus is the main appetite-regulating center in the brain receiving peripheral signals regarding the metabolic status of the body. Pancreatic procolipase has recently been identified in rat hypothalamus. Procolipase is known mainly for its actions in the intestine where it is cleaved to colipase, an enzyme required for the maintenance of pancreatic lipase activity, and enterostatin, a peptide involved in appetite regulation through the gut–brain axis. Colipase is able to increase the activity of pancreatic lipase-related protein-2 (PLRP2), a lipase also expressed in extra-pancreatic tissues. This study was performed to elucidate if PLRP2, in addition to colipase, is expressed in the hypothalamus and if the mRNAs of colipase and... (More)
The hypothalamus is the main appetite-regulating center in the brain receiving peripheral signals regarding the metabolic status of the body. Pancreatic procolipase has recently been identified in rat hypothalamus. Procolipase is known mainly for its actions in the intestine where it is cleaved to colipase, an enzyme required for the maintenance of pancreatic lipase activity, and enterostatin, a peptide involved in appetite regulation through the gut–brain axis. Colipase is able to increase the activity of pancreatic lipase-related protein-2 (PLRP2), a lipase also expressed in extra-pancreatic tissues. This study was performed to elucidate if PLRP2, in addition to colipase, is expressed in the hypothalamus and if the mRNAs of colipase and PLRP2 respond to metabolic challenges such as fasting, high-fat feeding or feeding sugar solutions. RNA from rat hypothalamus was extracted and subjected to RT-PCR. For quantitative mRNA analysis of hypothalamic tissue from the different metabolic situations real-time RT-PCR was used. We found PLRP2 and colipase mRNA to be expressed in the hypothalamus. An overnight fast resulted in down-regulated colipase (3-fold) and PLRP2 (7-fold) mRNA compared to freely fed rats. Conversely, high-fat feeding resulted in up-regulated colipase and PLRP2 mRNA (1.3-fold and 1.8-fold, respectively) compared to standard chow-fed rats. A similar up-regulation in mRNA expression was observed after offering sugar solutions. In conclusion, PLRP2 mRNA is expressed in the rat hypothalamus and both procolipase and PLRP-2 mRNA are down-regulated during fasting and up-regulated during conditions of metabolic excess, suggesting an involvement in signaling energy availability. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
High-fat diet, Colipase, PLRP2, Fasting, Hypothalamus, Sugar solutions
in
Brain Research
volume
1185
pages
152 - 157
publisher
Elsevier
external identifiers
  • wos:000252064400015
  • scopus:36448964501
ISSN
1872-6240
DOI
10.1016/j.brainres.2007.09.022
language
English
LU publication?
yes
id
93eed2a3-754c-4353-88d3-c5f3439ce577 (old id 608575)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17936733&dopt=Abstract
date added to LUP
2008-07-30 12:09:50
date last changed
2017-01-01 04:27:03
@article{93eed2a3-754c-4353-88d3-c5f3439ce577,
  abstract     = {The hypothalamus is the main appetite-regulating center in the brain receiving peripheral signals regarding the metabolic status of the body. Pancreatic procolipase has recently been identified in rat hypothalamus. Procolipase is known mainly for its actions in the intestine where it is cleaved to colipase, an enzyme required for the maintenance of pancreatic lipase activity, and enterostatin, a peptide involved in appetite regulation through the gut–brain axis. Colipase is able to increase the activity of pancreatic lipase-related protein-2 (PLRP2), a lipase also expressed in extra-pancreatic tissues. This study was performed to elucidate if PLRP2, in addition to colipase, is expressed in the hypothalamus and if the mRNAs of colipase and PLRP2 respond to metabolic challenges such as fasting, high-fat feeding or feeding sugar solutions. RNA from rat hypothalamus was extracted and subjected to RT-PCR. For quantitative mRNA analysis of hypothalamic tissue from the different metabolic situations real-time RT-PCR was used. We found PLRP2 and colipase mRNA to be expressed in the hypothalamus. An overnight fast resulted in down-regulated colipase (3-fold) and PLRP2 (7-fold) mRNA compared to freely fed rats. Conversely, high-fat feeding resulted in up-regulated colipase and PLRP2 mRNA (1.3-fold and 1.8-fold, respectively) compared to standard chow-fed rats. A similar up-regulation in mRNA expression was observed after offering sugar solutions. In conclusion, PLRP2 mRNA is expressed in the rat hypothalamus and both procolipase and PLRP-2 mRNA are down-regulated during fasting and up-regulated during conditions of metabolic excess, suggesting an involvement in signaling energy availability.},
  author       = {Rippe, Catarina and Erlanson-Albertsson, Charlotte and Lindqvist, Andreas},
  issn         = {1872-6240},
  keyword      = {High-fat diet,Colipase,PLRP2,Fasting,Hypothalamus,Sugar solutions},
  language     = {eng},
  pages        = {152--157},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Consequences of metabolic challenges on hypothalamic colipase and PLRP2 mRNA in rats.},
  url          = {http://dx.doi.org/10.1016/j.brainres.2007.09.022},
  volume       = {1185},
  year         = {2007},
}