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A comparison of complementary measures of vitamin B6 status, function, and metabolism in the European Prospective Investigation into Cancer and Nutrition (EPIC) study

Clasen, Joanna L. ; Heath, Alicia K. ; Van Puyvelde, Heleen ; Huybrechts, Inge ; Park, Jin Young ; Ferrari, Pietro ; Johansson, Mattias ; Scelo, Ghislaine ; Ulvik, Arve and Midttun, Øivind , et al. (2021) In American Journal of Clinical Nutrition 114(1). p.338-347
Abstract

Background: Vitamin B6 insufficiency has been linked to increased risk of cancer and other chronic diseases. The circulating concentration of pyridoxal 5-phosphate (PLP) is a commonly used measure of vitamin B6 status. Ratios of substrates indicating PLP coenzymatic function and metabolism may be useful complementary measures to further explore the role of vitamin B6 in health. Objectives: We explored the sensitivity of 5 outcomes, namely PLP concentration, homocysteine:cysteine (Hcy:Cys), cystathionine:cysteine (Cysta:Cys), the 3-hydroxykynurenine ratio (HKr), and the 4-pyridoxic acid ratio (PAr) to vitamin B6 intake as well as personal and lifestyle characteristics. Medthods: Dietary intake and biomarker data were collected from... (More)

Background: Vitamin B6 insufficiency has been linked to increased risk of cancer and other chronic diseases. The circulating concentration of pyridoxal 5-phosphate (PLP) is a commonly used measure of vitamin B6 status. Ratios of substrates indicating PLP coenzymatic function and metabolism may be useful complementary measures to further explore the role of vitamin B6 in health. Objectives: We explored the sensitivity of 5 outcomes, namely PLP concentration, homocysteine:cysteine (Hcy:Cys), cystathionine:cysteine (Cysta:Cys), the 3-hydroxykynurenine ratio (HKr), and the 4-pyridoxic acid ratio (PAr) to vitamin B6 intake as well as personal and lifestyle characteristics. Medthods: Dietary intake and biomarker data were collected from participants from 3 nested case-control studies within the European Prospective Investigation into Cancer and Nutrition (EPIC). Bayesian regression models assessed the associations of the 5 biomarker outcomes with vitamin B6 intake and personal and lifestyle covariates. Analogous models examined the relations of Hcy:Cys, Cysta:Cys, and HKr with PLP. Results: In total, 4608 participants were included in the analyses. Vitamin B6 intake was most strongly associated with PLP, moderately associated with Hcy:Cys, Cysta:Cys, and HKr, and not associated with PAr (fold change in marker given a doubling of vitamin B6 intake: PLP 1.60 [95% credible interval (CrI): 1.50, 1.71]; Hcy:Cys 0.87 [95% CrI: 0.84, 0.90]; Cysta:Cys 0.89 [95% CrI: 0.84, 0.94]; HKr 0.88 [95% CrI: 0.85, 0.91]; PAr 1.00 [95% CrI: 0.95, 1.05]). PAr was most sensitive to age, and HKr was least sensitive to BMI and alcohol intake. Sex and menopause status were strongly associated with all 5 markers. Conclusions: We found that 5 different markers, capturing different aspects of vitamin B6-related biological processes, varied in their associations with vitamin B6 intake and personal and lifestyle predictors.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dietary biomarkers, kynurenine pathway, PLP, transsulfuration pathway, vitamin B6
in
American Journal of Clinical Nutrition
volume
114
issue
1
pages
10 pages
publisher
Oxford University Press
external identifiers
  • pmid:33829249
  • scopus:85110734306
ISSN
0002-9165
DOI
10.1093/ajcn/nqab045
language
English
LU publication?
yes
id
63867c3a-5a44-4477-ba27-c3bde7cd739e
date added to LUP
2021-08-20 13:54:31
date last changed
2022-06-29 06:07:28
@article{63867c3a-5a44-4477-ba27-c3bde7cd739e,
  abstract     = {{<p>Background: Vitamin B6 insufficiency has been linked to increased risk of cancer and other chronic diseases. The circulating concentration of pyridoxal 5-phosphate (PLP) is a commonly used measure of vitamin B6 status. Ratios of substrates indicating PLP coenzymatic function and metabolism may be useful complementary measures to further explore the role of vitamin B6 in health. Objectives: We explored the sensitivity of 5 outcomes, namely PLP concentration, homocysteine:cysteine (Hcy:Cys), cystathionine:cysteine (Cysta:Cys), the 3-hydroxykynurenine ratio (HKr), and the 4-pyridoxic acid ratio (PAr) to vitamin B6 intake as well as personal and lifestyle characteristics. Medthods: Dietary intake and biomarker data were collected from participants from 3 nested case-control studies within the European Prospective Investigation into Cancer and Nutrition (EPIC). Bayesian regression models assessed the associations of the 5 biomarker outcomes with vitamin B6 intake and personal and lifestyle covariates. Analogous models examined the relations of Hcy:Cys, Cysta:Cys, and HKr with PLP. Results: In total, 4608 participants were included in the analyses. Vitamin B6 intake was most strongly associated with PLP, moderately associated with Hcy:Cys, Cysta:Cys, and HKr, and not associated with PAr (fold change in marker given a doubling of vitamin B6 intake: PLP 1.60 [95% credible interval (CrI): 1.50, 1.71]; Hcy:Cys 0.87 [95% CrI: 0.84, 0.90]; Cysta:Cys 0.89 [95% CrI: 0.84, 0.94]; HKr 0.88 [95% CrI: 0.85, 0.91]; PAr 1.00 [95% CrI: 0.95, 1.05]). PAr was most sensitive to age, and HKr was least sensitive to BMI and alcohol intake. Sex and menopause status were strongly associated with all 5 markers. Conclusions: We found that 5 different markers, capturing different aspects of vitamin B6-related biological processes, varied in their associations with vitamin B6 intake and personal and lifestyle predictors.</p>}},
  author       = {{Clasen, Joanna L. and Heath, Alicia K. and Van Puyvelde, Heleen and Huybrechts, Inge and Park, Jin Young and Ferrari, Pietro and Johansson, Mattias and Scelo, Ghislaine and Ulvik, Arve and Midttun, Øivind and Ueland, Per Magne and Dahm, Christina C. and Halkjær, Jytte and Olsen, Anja and Johnson, Theron and Katzke, Verena and Schulze, Matthias B. and Masala, Giovanna and Segrado, Francesco and De Magistris, Maria Santucci and Sacerdote, Carlotta and Ocké, Marga C. and Luján-Barroso, Leila and Ching-López, Ana and Huerta, José María and Ardanaz, Eva and Amiano, Pilar and Ericson, Ulrika and Manjer, Jonas and Gylling, Björn and Johansson, Ingegerd and Schmidt, Julie and Weiderpass, Elisabete and Riboli, Elio and Cross, Amanda J. and Muller, David C.}},
  issn         = {{0002-9165}},
  keywords     = {{dietary biomarkers; kynurenine pathway; PLP; transsulfuration pathway; vitamin B6}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{338--347}},
  publisher    = {{Oxford University Press}},
  series       = {{American Journal of Clinical Nutrition}},
  title        = {{A comparison of complementary measures of vitamin B6 status, function, and metabolism in the European Prospective Investigation into Cancer and Nutrition (EPIC) study}},
  url          = {{http://dx.doi.org/10.1093/ajcn/nqab045}},
  doi          = {{10.1093/ajcn/nqab045}},
  volume       = {{114}},
  year         = {{2021}},
}