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CRP in Outpatients with Inflammatory Bowel Disease Is Linked to the Blood Microbiota

Xu, Jie LU ; Molin, Göran LU ; Davidson, Sanna LU ; Roth, Bodil LU ; Sjöberg, Klas LU orcid and Håkansson, Åsa LU (2023) In International Journal of Molecular Sciences 24(13).
Abstract

The circulation is a closed system that has been assumed to be free from bacteria, but evidence for the existence of a low-density blood microbiota is accumulating. The present study aimed to map the blood microbiota of outpatients with Crohn’s disease (CD) or with ulcerative colitis (UC) by 16S metagenomics. A diverse microbiota was observed in the blood samples. Regardless of the type of disease, the alpha diversity of the microbiota was positively associated with C-reactive protein (CRP). The blood microbiota had a surprisingly high proportion of Proteobacteria in comparison with human oral and colonic microbiotas. There was no clear difference in the overall pattern of the microbiota between CD and UC. A non-template control (NTC)... (More)

The circulation is a closed system that has been assumed to be free from bacteria, but evidence for the existence of a low-density blood microbiota is accumulating. The present study aimed to map the blood microbiota of outpatients with Crohn’s disease (CD) or with ulcerative colitis (UC) by 16S metagenomics. A diverse microbiota was observed in the blood samples. Regardless of the type of disease, the alpha diversity of the microbiota was positively associated with C-reactive protein (CRP). The blood microbiota had a surprisingly high proportion of Proteobacteria in comparison with human oral and colonic microbiotas. There was no clear difference in the overall pattern of the microbiota between CD and UC. A non-template control (NTC) was included in the whole process to control for the potential contamination from the environment and reagents. Certain bacterial taxa were concomitantly detected in both blood samples and NTC. However, Acinetobacter, Lactobacillus, Thermicanus and Paracoccus were found in blood from both CD and UC patients but not in NTC, indicating the existence of a specific blood-borne microbiota in the patients. Achromobacter dominated in all blood samples, but a minor amount was also found in NTC. Micrococcaceae was significantly enriched in CD, but it was also detected in high abundance in NTC. Whether the composition of the blood microbiota could be a marker of a particular phenotype in inflammatory bowel disease (IBD) or whether the blood microbiota could be used for diagnostic or therapeutic purposes deserves further attention.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Achromobacter, blood, Crohn’s disease, CRP, diversity, IBD, microbiota, ulcerative colitis
in
International Journal of Molecular Sciences
volume
24
issue
13
article number
10899
publisher
MDPI AG
external identifiers
  • pmid:37446076
  • scopus:85164846252
ISSN
1661-6596
DOI
10.3390/ijms241310899
language
English
LU publication?
yes
id
65ee0682-5b0d-4952-92d1-016a6424856f
date added to LUP
2023-09-15 08:05:40
date last changed
2024-04-20 03:18:00
@article{65ee0682-5b0d-4952-92d1-016a6424856f,
  abstract     = {{<p>The circulation is a closed system that has been assumed to be free from bacteria, but evidence for the existence of a low-density blood microbiota is accumulating. The present study aimed to map the blood microbiota of outpatients with Crohn’s disease (CD) or with ulcerative colitis (UC) by 16S metagenomics. A diverse microbiota was observed in the blood samples. Regardless of the type of disease, the alpha diversity of the microbiota was positively associated with C-reactive protein (CRP). The blood microbiota had a surprisingly high proportion of Proteobacteria in comparison with human oral and colonic microbiotas. There was no clear difference in the overall pattern of the microbiota between CD and UC. A non-template control (NTC) was included in the whole process to control for the potential contamination from the environment and reagents. Certain bacterial taxa were concomitantly detected in both blood samples and NTC. However, Acinetobacter, Lactobacillus, Thermicanus and Paracoccus were found in blood from both CD and UC patients but not in NTC, indicating the existence of a specific blood-borne microbiota in the patients. Achromobacter dominated in all blood samples, but a minor amount was also found in NTC. Micrococcaceae was significantly enriched in CD, but it was also detected in high abundance in NTC. Whether the composition of the blood microbiota could be a marker of a particular phenotype in inflammatory bowel disease (IBD) or whether the blood microbiota could be used for diagnostic or therapeutic purposes deserves further attention.</p>}},
  author       = {{Xu, Jie and Molin, Göran and Davidson, Sanna and Roth, Bodil and Sjöberg, Klas and Håkansson, Åsa}},
  issn         = {{1661-6596}},
  keywords     = {{Achromobacter; blood; Crohn’s disease; CRP; diversity; IBD; microbiota; ulcerative colitis}},
  language     = {{eng}},
  number       = {{13}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{CRP in Outpatients with Inflammatory Bowel Disease Is Linked to the Blood Microbiota}},
  url          = {{http://dx.doi.org/10.3390/ijms241310899}},
  doi          = {{10.3390/ijms241310899}},
  volume       = {{24}},
  year         = {{2023}},
}