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Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes

Ramakers, Julian D.; Volman, Julia J.; Biörklund, Maria LU ; Onning, Gunilla; Mensink, Ronald P. and Plat, Jogchum (2007) In Molecular Nutrition and Food Research 51(2). p.211-220
Abstract
Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were... (More)
Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-glucan, oat, intestinal epithelial cells, interleukin-8, ICAM-1, ileostomy
in
Molecular Nutrition and Food Research
volume
51
issue
2
pages
211 - 220
publisher
John Wiley & Sons
external identifiers
  • wos:000245098700007
  • scopus:34250792671
ISSN
1613-4133
DOI
10.1002/mnfr.200600149
language
English
LU publication?
yes
id
c7327abb-e1fe-404e-887a-75a909cfe395 (old id 669589)
date added to LUP
2007-12-14 09:55:16
date last changed
2017-10-08 03:42:25
@article{c7327abb-e1fe-404e-887a-75a909cfe395,
  abstract     = {Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation.},
  author       = {Ramakers, Julian D. and Volman, Julia J. and Biörklund, Maria and Onning, Gunilla and Mensink, Ronald P. and Plat, Jogchum},
  issn         = {1613-4133},
  keyword      = {beta-glucan,oat,intestinal epithelial cells,interleukin-8,ICAM-1,ileostomy},
  language     = {eng},
  number       = {2},
  pages        = {211--220},
  publisher    = {John Wiley & Sons},
  series       = {Molecular Nutrition and Food Research},
  title        = {Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes},
  url          = {http://dx.doi.org/10.1002/mnfr.200600149},
  volume       = {51},
  year         = {2007},
}