Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes
(2007) In Molecular Nutrition and Food Research 51(2). p.211-220- Abstract
- Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were... (More)
- Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/669589
- author
- Ramakers, Julian D. ; Volman, Julia J. ; Biörklund, Maria LU ; Onning, Gunilla ; Mensink, Ronald P. and Plat, Jogchum
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- beta-glucan, oat, intestinal epithelial cells, interleukin-8, ICAM-1, ileostomy
- in
- Molecular Nutrition and Food Research
- volume
- 51
- issue
- 2
- pages
- 211 - 220
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000245098700007
- scopus:34250792671
- ISSN
- 1613-4133
- DOI
- 10.1002/mnfr.200600149
- language
- English
- LU publication?
- yes
- id
- c7327abb-e1fe-404e-887a-75a909cfe395 (old id 669589)
- date added to LUP
- 2016-04-01 12:30:34
- date last changed
- 2022-03-21 05:16:39
@article{c7327abb-e1fe-404e-887a-75a909cfe395,
abstract = {{Yeast, fungal, and dietary beta-glucans have immune-modulating effects in vitro and in vivo, as thought, mainly by affecting leukocytes; however, effects of oat beta-glucan on enterocytes have never been studied. As recognized, supplying oat beta-glucans as such to cells in culture directly is difficult because of solubility problems. Therefore, six ileostomic patients consumed, in random order, a control diet or an oat beta-glucan enriched diet (5 g) and from the collected ileostomic content, fecal water was prepared and added to two small intestinal cell lines (INT407, Caco-2) and two colon cell lines (HT29, T84) together with a cytokine cocktail (IL-IP + 1NF gamma + TNF alpha). Several parameters reflecting immune-modulation were measured. As compared to placebo fecal water, P-glucan enriched fecal water significantly increased IL-8 production in HT29 (5.0%; p = 0.046) and INT407 cells (22.0%; p=0.028). Intercellular adhesion molecule (ICAM)-1 expression increased in T84 (11.0%; p = 0.028) and Caco-2 cells (20.4%; p = 0.075). These immune-stimulating effects were confirmed by enhancement of inflammatory expression profiles, as determined with an antibody array. Our findings show immune enhancement by fecal water from ileostomic patients consuming oat P-glucan both in small intestinal and colon cell lines after stimulation, which is in agreement with documented effects in leukocytes. Whether these immune-stimulating effects on enterocytes contribute to the enhanced protection of the host against invading pathogens as observed both in animals and in humans, as well as the underlying mechanism, needs further evaluation.}},
author = {{Ramakers, Julian D. and Volman, Julia J. and Biörklund, Maria and Onning, Gunilla and Mensink, Ronald P. and Plat, Jogchum}},
issn = {{1613-4133}},
keywords = {{beta-glucan; oat; intestinal epithelial cells; interleukin-8; ICAM-1; ileostomy}},
language = {{eng}},
number = {{2}},
pages = {{211--220}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Molecular Nutrition and Food Research}},
title = {{Fecal water from ileostomic patients consuming oat beta-glucan enhances immune responses in enterocytes}},
url = {{http://dx.doi.org/10.1002/mnfr.200600149}},
doi = {{10.1002/mnfr.200600149}},
volume = {{51}},
year = {{2007}},
}