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Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell, Per ; Wilkinson, Mark ; Taylor, Gavin J. LU orcid ; Hall, Stephen LU ; Sharma, Shrikant ; Sirijovski, Nick LU ; Hansson, Mats LU ; Shewry, Peter R. ; Hofvander, Per and Grimberg, Åsa (2022) In Plants 11(7).
Abstract

Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had... (More)

Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the AsWRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
aleurone cells, carbon allocation, cereal endosperm, geneoneinserts, line withone onelineinsert, oil biosynthesis, the control).and theComparisoncontrol). Comparisonof sections of caryopsessections ofaftercaryop-fixation, transcription factor, triacylglycerol, Triticum aestivum, wheat flour, with oneandinsert, WRINKLED1
in
Plants
volume
11
issue
7
article number
889
publisher
MDPI AG
external identifiers
  • pmid:35406869
  • scopus:85127059350
ISSN
2223-7747
DOI
10.3390/plants11070889
language
English
LU publication?
yes
id
66dfc934-e8e7-4ec2-ac85-ef079719d648
date added to LUP
2022-06-03 14:36:58
date last changed
2024-04-18 14:46:27
@article{66dfc934-e8e7-4ec2-ac85-ef079719d648,
  abstract     = {{<p>Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the AsWRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.</p>}},
  author       = {{Snell, Per and Wilkinson, Mark and Taylor, Gavin J. and Hall, Stephen and Sharma, Shrikant and Sirijovski, Nick and Hansson, Mats and Shewry, Peter R. and Hofvander, Per and Grimberg, Åsa}},
  issn         = {{2223-7747}},
  keywords     = {{aleurone cells; carbon allocation; cereal endosperm; geneoneinserts; line withone onelineinsert; oil biosynthesis; the control).and theComparisoncontrol). Comparisonof sections of caryopsessections ofaftercaryop-fixation; transcription factor; triacylglycerol; Triticum aestivum; wheat flour; with oneandinsert; WRINKLED1}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{MDPI AG}},
  series       = {{Plants}},
  title        = {{Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1}},
  url          = {{http://dx.doi.org/10.3390/plants11070889}},
  doi          = {{10.3390/plants11070889}},
  volume       = {{11}},
  year         = {{2022}},
}