Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations
(2022) In Stem Cell Reports 17(12). p.2718-2731- Abstract
The respiratory epithelium consists of multiple, functionally distinct cell types and is maintained by regionally specific progenitor populations that repair the epithelium following injury. Several in vitro methods exist for studying lung epithelial repair using primary murine lung cells, but isolation methods are hampered by a lack of surface markers distinguishing epithelial progenitors along the respiratory epithelium. Here, we developed a 3D printed lobe divider (3DLD) to aid in simultaneous isolation of proximal versus distal lung epithelial progenitors from individual mice that give rise to differentiated epithelia in multiple in vitro assays. In contrast to 3DLD-isolated distal progenitor cells, commonly used manual tracheal... (More)
The respiratory epithelium consists of multiple, functionally distinct cell types and is maintained by regionally specific progenitor populations that repair the epithelium following injury. Several in vitro methods exist for studying lung epithelial repair using primary murine lung cells, but isolation methods are hampered by a lack of surface markers distinguishing epithelial progenitors along the respiratory epithelium. Here, we developed a 3D printed lobe divider (3DLD) to aid in simultaneous isolation of proximal versus distal lung epithelial progenitors from individual mice that give rise to differentiated epithelia in multiple in vitro assays. In contrast to 3DLD-isolated distal progenitor cells, commonly used manual tracheal ligation methods followed by lobe removal resulted in co-isolation of rare proximal cells with distal cells, which altered the transcriptional landscape and size distribution of distal organoids. The 3DLD aids in reproducible isolation of distal versus proximal progenitor populations and minimizes the potential for contaminating populations to confound in vitro assays.
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- author
- Alsafadi, Hani N LU ; Stegmayr, John LU ; Ptasinski, Victoria LU ; Silva, Iran LU ; Mittendorfer, Margareta LU ; Murray, Lynne A and Wagner, Darcy E LU
- organization
-
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Lung Bioengineering and Regeneration (research group)
- WCMM-Wallenberg Centre for Molecular Medicine
- Thoracic Surgery
- LTH Profile Area: Nanoscience and Semiconductor Technology
- NanoLund: Centre for Nanoscience
- LTH Profile Area: Engineering Health
- publishing date
- 2022-11-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Reports
- volume
- 17
- issue
- 12
- pages
- 2718 - 2731
- publisher
- Cell Press
- external identifiers
-
- pmid:36460000
- scopus:85143875009
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2022.11.002
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
- id
- 680b88cc-0d31-40be-ab19-db90aa2688c5
- date added to LUP
- 2022-12-04 20:12:47
- date last changed
- 2024-04-04 13:26:00
@article{680b88cc-0d31-40be-ab19-db90aa2688c5, abstract = {{<p>The respiratory epithelium consists of multiple, functionally distinct cell types and is maintained by regionally specific progenitor populations that repair the epithelium following injury. Several in vitro methods exist for studying lung epithelial repair using primary murine lung cells, but isolation methods are hampered by a lack of surface markers distinguishing epithelial progenitors along the respiratory epithelium. Here, we developed a 3D printed lobe divider (3DLD) to aid in simultaneous isolation of proximal versus distal lung epithelial progenitors from individual mice that give rise to differentiated epithelia in multiple in vitro assays. In contrast to 3DLD-isolated distal progenitor cells, commonly used manual tracheal ligation methods followed by lobe removal resulted in co-isolation of rare proximal cells with distal cells, which altered the transcriptional landscape and size distribution of distal organoids. The 3DLD aids in reproducible isolation of distal versus proximal progenitor populations and minimizes the potential for contaminating populations to confound in vitro assays.</p>}}, author = {{Alsafadi, Hani N and Stegmayr, John and Ptasinski, Victoria and Silva, Iran and Mittendorfer, Margareta and Murray, Lynne A and Wagner, Darcy E}}, issn = {{2213-6711}}, language = {{eng}}, month = {{11}}, number = {{12}}, pages = {{2718--2731}}, publisher = {{Cell Press}}, series = {{Stem Cell Reports}}, title = {{Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations}}, url = {{http://dx.doi.org/10.1016/j.stemcr.2022.11.002}}, doi = {{10.1016/j.stemcr.2022.11.002}}, volume = {{17}}, year = {{2022}}, }